Research progress in the construction of nucleic acid and protein biomolecular sensor arrays and their applications for rapid detection

NI Weiwei; ZHOU Lingjia; WANG Hao; LI Fei; HAN Jinsong

doi:10.12211/2096-8280.2022-035

您当前的位置：

首页 >

文章列表页 >

Research progress in the construction of nucleic acid and protein biomolecular sensor arrays and their applications for rapid detection

Invited Review | 更新时间：2025-03-20

- Research progress in the construction of nucleic acid and protein biomolecular sensor arrays and their applications for rapid detection
- Synthetic Biology Journal Vol. 4, Issue 1, Pages: 185-203(2023)
- 作者机构：
  
  中国药科大学工学院食品质量与安全系，天然药物活性组分与药效国家重点实验室，国家中药材加工研发专业中心，江苏南京 211109
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2022-035
  CLC： Q816
- Received：25 June 2022，
  
  Revised：2022-08-26，
  
  Published：28 February 2023
- 稿件说明：
移动端阅览
倪伟伟，周玲佳，王浩，李飞，韩进松. 基于核酸和蛋白质生物分子阵列传感器的构建及检测应用的研究进展［J］. 合成生物学， 2023， 4（1）： 185-203

NI Weiwei， ZHOU Lingjia， WANG Hao， LI Fei， HAN Jinsong. Research progress in the construction of nucleic acid and protein biomolecular sensor arrays and their applications for rapid detection［J］. Synthetic Biology Journal， 2023， 4（1）： 185-203
倪伟伟，周玲佳，王浩，李飞，韩进松. 基于核酸和蛋白质生物分子阵列传感器的构建及检测应用的研究进展［J］. 合成生物学， 2023， 4（1）： 185-203 DOI： 10.12211/2096-8280.2022-035.

NI Weiwei， ZHOU Lingjia， WANG Hao， LI Fei， HAN Jinsong. Research progress in the construction of nucleic acid and protein biomolecular sensor arrays and their applications for rapid detection［J］. Synthetic Biology Journal， 2023， 4（1）： 185-203 DOI： 10.12211/2096-8280.2022-035.

摘要

阵列传感器是组合多个传感元件，通过交叉反应机理来区分一类目标分析物的策略，不同于传统探针类传感器“锁-钥”结合模式，阵列传感器为非特异性结合，可实现一对多的识别。然而，传感元件的制备仍然存在设计困难、合成复杂、信号转换效率低等诸多挑战。核酸和蛋白具有优良的生物相容性、灵活的可编程性、易实现的功能化和优越的分子识别性质等优点。目前已经通过合理设计和可控化制备手段构建了多种基于核酸和蛋白类传感元件的阵列传感器，结合机器学习算法对所得数据进行处理，使数据可视化，构建待测物“指纹图谱”，对待测物进行区分。在此综述中，重点介绍了基于核酸和蛋白类传感元件构建的阵列传感器的多目标检测应用，并讨论了其合理的应用前景以及挑战。

Abstract

The advent of sensor arrays is inspired by the superior performance of biological olfactory and taste systems on detection

recognition

tracking

and localization. By simulating the sensing mechanism of cross-response with the olfactory and taste systems

a sensor array can be constructed. Meanwhile

a series of algorithms are used to detect and distinguish targets based on fingerprints generated by the analyte to the array. However

the fabrication of sensing elements still faces many challenges

such as difficult design

complicated synthesis

and low signal collecting efficiency. Nucleic acids and proteins have advantages in biocompatibility

flexible programmability

easy functionalization

and superior molecular recognition properties. At present

a variety of sensor arrays based on nucleic acid and protein sensing elements have been constructed through rational design and controllable preparation methods. In this review

the multi-target detection applications of these sensor arrays are highlighted

and their application prospects and challenges are addressed.

关键词

Keywords

references

CHEN J H , ANDLER S M , GODDARD J M , et al . Integrating recognition elements with nanomaterials for bacteria sensing [J ] . Chemical Society Reviews , 2017 , 46 ( 5 ): 1272 - 1283 .

YOU L , ZHA D J , ANSLYN E V . Recent advances in supramolecular analytical chemistry using optical sensing [J ] . Chemical Reviews , 2015 , 115 ( 15 ): 7840 - 7892 .

LIN M , LI W S , WANG Y N , et al . Discrimination of hemoglobins with subtle differences using an aptamer based sensing array [J ] . Chemical Communications , 2015 , 51 ( 39 ): 8304 - 8306 .

GALE P A , CALTAGIRONE C . Anion sensing by small molecules and molecular ensembles [J ] . Chemical Society Reviews , 2015 , 44 ( 13 ): 4212 - 4227 .

SUN J W , LU Y X , HE L Y , et al . Colorimetric sensor array based on gold nanoparticles: design principles and recent advances [J ] . TrAC Trends in Analytical Chemistry , 2020 , 122 : 115754 .

PU F , RAN X , GUAN M , et al . Biomolecule-templated photochemical synthesis of silver nanoparticles: multiple readouts of localized surface plasmon resonance for pattern recognition [J ] . Nano Research , 2018 , 11 ( 6 ): 3213 - 3221 .

PU F , RAN X , REN J S , et al . Artificial tongue based on metal-biomolecule coordination polymer nanoparticles [J ] . Chemical Communications , 2016 , 52 ( 16 ): 3410 - 3413 .

ZHOU W H , SARAN R , LIU J W . Metal sensing by DNA [J ] . Chemical Reviews , 2017 , 117 ( 12 ): 8272 - 8325 .

ZHENG J , YANG R H , SHI M L , et al . Rationally designed molecular beacons for bioanalytical and biomedical applications [J ] . Chemical Society Reviews , 2015 , 44 ( 10 ): 3036 - 3055 .

TAN W H , DONOVAN M J , JIANG J H . Aptamers from cell-based selection for bioanalytical applications [J ] . Chemical Reviews , 2013 , 113 ( 4 ): 2842 - 2862 .

BELL N M , MICKLEFIELD J . Chemical modification of oligonucleotides for therapeutic, bioanalytical and other applications [J ] . ChemBioChem , 2009 , 10 ( 17 ): 2691 - 2703 .

HU L , XU J , QIN Z , et al . Detection of bitterness in vitro by a novel male mouse germ cell-based biosensor [J ] . Sensors and Actuators B: Chemical , 2016 , 223 : 461 - 469 .

LI L , XU S J , YAN H , et al . Nucleic acid aptamers for molecular diagnostics and therapeutics: advances and perspectives [J ] . Angewandte Chemie International Edition , 2021 , 60 ( 5 ): 2221 - 2231 .

MOUTSIOPOULOU A , BROYLES D , DIKICI E , et al . Molecular aptamer beacons and their applications in sensing, imaging, and diagnostics [J ] . Small , 2019 , 15 ( 35 ): 1902248 .

BAMRUNGSAP S , CHEN T , SHUKOOR M I , et al . Pattern recognition of cancer cells using aptamer-conjugated magnetic nanoparticles [J ] . ACS Nano , 2012 , 6 ( 5 ): 3974 - 3981 .

WANG S , KONG H , GONG X Y , et al . Multicolor imaging of cancer cells with fluorophore-tagged aptamers for single cell typing [J ] . Analytical Chemistry , 2014 , 86 ( 16 ): 8261 - 8266 .

TOMITA S , SUGAI H , MIMURA M , et al . Optical fingerprints of proteases and their inhibited complexes provided by differential cross-reactivity of fluorophore-labeled single-stranded DNA [J ] . ACS Applied Materials & Interfaces , 2019 , 11 ( 50 ): 47428 - 47436 .

LI Z , ASKIM J R , SUSLICK K S . The optoelectronic nose: colorimetric and fluorometric sensor arrays [J ] . Chemical Reviews , 2019 , 119 ( 1 ): 231 - 292 .

OKADA M , SUGAI H , TOMITA S , et al . A multichannel pattern-recognition-based protein sensor with a fluorophore-conjugated single-stranded DNA set [J ] . Sensors , 2020 , 20 ( 18 ): 5110 .

SIGEL H . Interactions of metal ions with nucleotides and nucleic acids and their constituents [J ] . Chemical Society Reviews , 1993 , 22 ( 4 ): 255 - 267 .

QIU H , PU F , RAN X , et al . Fingerprint-like pattern for recognition of thiols [J ] . Sensors and Actuators B: Chemical , 2018 , 260 : 183 - 188 .

XUE S F , CHEN Z H , HAN X Y , et al . DNA encountering terbium(Ⅲ): a smart "Chemical nose/tongue" for large-scale time-gated luminescent and lifetime-based sensing [J ] . Analytical Chemistry , 2018 , 90 ( 5 ): 3443 - 3451 .

XUE S F , HAN X Y , CHEN Z H , et al . The chemistry of europium(Ⅲ) encountering DNA: sprouting unique sequence-dependent performances for multifunctional time-resolved luminescent assays [J ] . Analytical Chemistry , 2018 , 90 ( 17 ): 10614 - 10620 .

WU S , HAN Y W , WANG L , et al . Sensor array fabricated with nanoscale metal-organic frameworks for the histopathological examination of colon cancer [J ] . Analytical Chemistry , 2019 , 91 ( 16 ): 10772 - 10778 .

HIZIR M S , ROBERTSON N M , BALCIOGLU M , et al . Universal sensor array for highly selective system identification using two-dimensional nanoparticles [J ] . Chemical Science , 2017 , 8 ( 8 ): 5735 - 5745 .

HIZIR M S , NANDU N , YIGIT M V . Homologous miRNA analyses using a combinatorial nanosensor array with two-dimensional nanoparticles [J ] . Analytical Chemistry , 2018 , 90 ( 10 ): 6300 - 6306 .

NANDU N , HIZIR M S , YIGIT M V . Systematic investigation of two-dimensional DNA nanoassemblies for construction of a nonspecific sensor array [J ] . Langmuir , 2018 , 34 ( 49 ): 14983 - 14992 .

AUGSPURGER E E , RANA M , YIGIT M V . Chemical and biological sensing using hybridization chain reaction [J ] . ACS Sensors , 2018 , 3 ( 5 ): 878 - 902 .

QI J W , RAO P H , WANG L L , et al . Development of pattern recognition based on nanosheet-DNA probes and an extendable DNA library [J ] . Analyst , 2021 , 146 ( 15 ): 4803 - 4810 .

LIU B W , LIU J W . Comprehensive screen of metal oxide nanoparticles for DNA adsorption, fluorescence quenching, and anion discrimination [J ] . ACS Applied Materials & Interfaces , 2015 , 7 ( 44 ): 24833 - 24838 .

PLA L , SANTIAGO-FELIPE S , TORMO-MAS M Á , et al . Aptamer-capped nanoporous anodic alumina for staphylococcus aureus detection [J ] . Sensors and Actuators B: Chemical , 2020 , 320 : 128281 .

LU Y X , LIU Y Y , ZHANG S G , et al . Aptamer-based plasmonic sensor array for discrimination of proteins and cells with the naked eye [J ] . Analytical Chemistry , 2013 , 85 ( 14 ): 6571 - 6574 .

WEI X C , WANG Y X , ZHAO Y X , et al . Colorimetric sensor array for protein discrimination based on different DNA chain length-dependent gold nanoparticles aggregation [J ] . Biosensors and Bioelectronics , 2017 , 97 : 332 - 337 .

YAN S , LAI X X , DU G R , et al . Identification of aminoglycoside antibiotics in milk matrix with a colorimetric sensor array and pattern recognition methods [J ] . Analytica Chimica Acta , 2018 , 1034 : 153 - 160 .

WANG F Y , ZHANG X , LU Y X , et al . Continuously evolving 'chemical tongue' biosensor for detecting proteins [J ] . Talanta , 2017 , 165 : 182 - 187 .

JIA F F , LIU Q Y , WEI W , et al . Colorimetric sensor assay for discrimination of proteins based on exonuclease Ⅰ-triggered aggregation of DNA-functionalized gold nanoparticles [J ] . Analyst , 2019 , 144 ( 16 ): 4865 - 4870 .

XI H Y , HE W W , LIU Q Y , et al . Protein discrimination using a colorimetric sensor array based on gold nanoparticle aggregation induced by cationic polymer [J ] . ACS Sustainable Chemistry & Engineering , 2018 , 6 ( 8 ): 10751 - 10757 .

YANG X F , LI J , PEI H , et al . Pattern recognition analysis of proteins using DNA-decorated catalytic gold nanoparticles [J ] . Small , 2013 , 9 ( 17 ): 2844 - 2849 .

YANG X F , LI J , PEI H , et al . DNA-gold nanoparticle conjugates-based nanoplasmonic probe for specific differentiation of cell types [J ] . Analytical Chemistry , 2014 , 86 ( 6 ): 3227 - 3231 .

LI C , YANG Y C , WEI L M , et al . An array-based approach to determine different subtype and differentiation of non-small cell lung cancer [J ] . Theranostics , 2015 , 5 ( 1 ): 62 - 70 .

SUN Z W , WU S , MA J H , et al . Colorimetric sensor array for human semen identification designed by coupling zirconium metal-organic frameworks with DNA-modified gold nanoparticles [J ] . ACS Applied Materials & Interfaces , 2019 , 11 ( 40 ): 36316 - 36323 .

WEI X C , CHEN Z B , TAN L L , et al . DNA-catalytically active gold nanoparticle conjugates-based colorimetric multidimensional sensor array for protein discrimination [J ] . Analytical Chemistry , 2017 , 89 ( 1 ): 556 - 559 .

YANG J E , LU Y X , AO L , et al . Colorimetric sensor array for proteins discrimination based on the tunable peroxidase-like activity of AuNPs-DNA conjugates [J ] . Sensors and Actuators B: Chemical , 2017 , 245 : 66 - 73 .

SUN W B , LU Y X , MAO J P , et al . Multidimensional sensor for pattern recognition of proteins based on DNA-gold nanoparticles conjugates [J ] . Analytical Chemistry , 2015 , 87 ( 6 ): 3354 - 3359 .

DAS SAHA N , SASMAL R , MEETHAL S K , et al . Multichannel DNA sensor array fingerprints cell states and identifies pharmacological effectors of catabolic processes [J ] . ACS Sensors , 2019 , 4 ( 12 ): 3124 - 3132 .

TAN L L , CHEN Z B , ZHAO Y . Dual channel sensor for detection and discrimination of heavy metal ions based on colorimetric and fluorescence response of the AuNPs-DNA conjugates [J ] . Biosensors and Bioelectronics , 2016 , 85 : 414 - 421 .

ZHOU L , REN J S , QU X G . Nucleic acid-templated functional nanocomposites for biomedical applications [J ] . Materials Today , 2017 , 20 ( 4 ): 179 - 190 .

KUMAR A , KUMAR V . Biotemplated inorganic nanostructures: supramolecular directed nanosystems of semiconductor(s)/metal(s) mediated by nucleic acids and their properties [J ] . Chemical Reviews , 2014 , 114 ( 14 ): 7044 - 7078 .

XU F Z , QING T P , QING Z H . DNA-coded metal nano-fluorophores: preparation, properties and applications in biosensing and bioimaging [J ] . Nano Today , 2021 , 36 : 101021 .

CHEN Y X , PHIPPS M L , WERNER J H , et al . DNA templated metal nanoclusters: from emergent properties to unique applications [J ] . Accounts of Chemical Research , 2018 , 51 ( 11 ): 2756 - 2763 .

XI H Y , LI X , LIU Q Y , et al . Fluorescent sensor array for discrimination of biothiols based on poly(thymine/cytosine)-templated copper nanoparticles [J ] . Analytica Chimica Acta , 2019 , 1051 : 147 - 152 .

QING Z H , HE X X , HE D G , et al . Poly(thymine)-templated selective formation of fluorescent copper nanoparticles [J ] . Angewandte Chemie , 2013 , 125 ( 37 ): 9901 - 9904 .

CLEVER G , KAUL C , CARELL T . DNA-metal base pairs [J ] . Angewandte Chemie International Edition , 2007 , 46 ( 33 ): 6226 - 6236 .

CHUNG C , KIM Y K , SHIN D , et al . Biomedical applications of graphene and graphene oxide [J ] . Accounts of Chemical Research , 2013 , 46 ( 10 ): 2211 - 2224 .

PEI H , LI J , LV M , et al . A graphene-based sensor array for high-precision and adaptive target identification with ensemble aptamers [J ] . Journal of the American Chemical Society , 2012 , 134 ( 33 ): 13843 - 13849 .

TOMITA S , MATSUDA A , NISHINAMI S , et al . One-step identification of antibody degradation pathways using fluorescence signatures generated by cross-reactive DNA-based arrays [J ] . Analytical Chemistry , 2017 , 89 ( 15 ): 7818 - 7822 .

TOMITA S , ISHIHARA S , KURITA R . A multi-fluorescent DNA/graphene oxide conjugate sensor for signature-based protein discrimination [J ] . Sensors , 2017 , 17 ( 10 ): 2194 .

WU S J , ZHANG H , SHI Z . Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles [J ] . Food Control , 2015 , 50 : 597 - 604 .

SUN Y H , DUAN N , MA P F , et al . Colorimetric aptasensor based on truncated aptamer and trivalent DNAzyme for Vibrio parahemolyticus determination [J ] . Journal of Agricultural and Food Chemistry , 2019 , 67 ( 8 ): 2313 - 2320 .

GUO Y Y , ZHAO C , LIU Y S , et al . A novel fluorescence method for the rapid and effective detection of Listeria monocytogenes using aptamer-conjugated magnetic nanoparticles and aggregation-induced emission dots [J ] . Analyst , 2020 , 145 ( 11 ): 3857 - 3863 .

QIU H , PU F , RAN X , et al . Nanozyme as artificial receptor with multiple readouts for pattern recognition [J ] . Analytical Chemistry , 2018 , 90 ( 20 ): 11775 - 11779 .

LIU M X , ZHANG H , ZHANG X W , et al . Nanozyme sensor array plus solvent-mediated signal amplification strategy for ultrasensitive ratiometric fluorescence detection of exosomal proteins and cancer identification [J ] . Analytical Chemistry , 2021 , 93 ( 25 ): 9002 - 9010 .

LI H P , BAZAN G C . Conjugated oligoelectrolyte/ssDNA aggregates: self-assembled multicomponent chromophores for protein discrimination [J ] . Advanced Materials , 2009 , 21 ( 9 ): 964 - 967 .

ZHOU W , HOU J Z , LI Y X , et al . Protein discrimination based on DNA induced perylene probe self-assembly [J ] . Talanta , 2021 , 224 : 121897 .

DUARTE A , CHWOROS A , FLAGAN S F , et al . Identification of bacteria by conjugated oligoelectrolyte/single-stranded DNA electrostatic complexes [J ] . Journal of the American Chemical Society , 2010 , 132 ( 36 ): 12562 - 12564 .

QIU H , PU F , RAN X , et al . A DNA-based label-free artificial tongue for pattern recognition of metal ions [J ] . Chemistry-A European Journal , 2017 , 23 ( 39 ): 9258 - 9261 .

LI L , LIU B , CHEN Z B . Heavy metal ion discrimination based on distinct interaction between single-stranded DNA and methylene blue [J ] . Analytical Methods , 2019 , 11 ( 1 ): 17 - 20 .

EUBANKS C S , FORTE J E , KAPRAL G J , et al . Small molecule-based pattern recognition to classify RNA structure [J ] . Journal of the American Chemical Society , 2017 , 139 ( 1 ): 409 - 416 .

EUBANKS C S , ZHAO B , PATWARDHAN N N , et al . Visualizing RNA conformational changes via pattern recognition of RNA by small molecules [J ] . Journal of the American Chemical Society , 2019 , 141 ( 14 ): 5692 - 5698 .

EUBANKS C S , HARGROVE A E . RNA structural differentiation: opportunities with pattern recognition [J ] . Biochemistry , 2019 , 58 ( 4 ): 199 - 213 .

EUBANKS C S , HARGROVE A E . Sensing the impact of environment on small molecule differentiation of RNA sequences [J ] . Chemical Communications , 2017 , 53 ( 100 ): 13363 - 13366 .

ZUFFO M , XIE X , GRANZHAN A . Strength in numbers: development of a fluorescence sensor array for secondary structures of DNA [J ] . Chemistry-A European Journal , 2019 , 25 ( 7 ): 1812 - 1818 .

ZUFFO M , GANDOLFINI A , HEDDI B , et al . Harnessing intrinsic fluorescence for typing of secondary structures of DNA [J ] . Nucleic Acids Research , 2020 , 48 ( 11 ): e61 .

DEL VILLAR-GUERRA R , GRAY R D , TRENT J O , et al . A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand-nucleic acid structural selectivity [J ] . Nucleic Acids Research , 2018 , 46 ( 7 ): e41 .

LIU Q J , WU C S , CAI H , et al . Cell-based biosensors and their application in biomedicine [J ] . Chemical Reviews , 2014 , 114 ( 12 ): 6423 - 6461 .

WU C S , DU L P , WANG D , et al . A biomimetic olfactory-based biosensor with high efficiency immobilization of molecular detectors [J ] . Biosensors and Bioelectronics , 2012 , 31 ( 1 ): 44 - 48 .

庄柳静 , 周俊 , 董琪 , 等 . 利用动物嗅觉诊断癌症技术的研究进展 [J ] . 科学通报 , 2013 , 58 ( 15 ): 1369 - 1378 .

ZHUANG L J , ZHOU J , DONG Q , et al . The research progress of using the mammalian olfaction for cancer diagnosis [J ] . Chinese Science Bulletin , 2013 , 58 ( 15 ): 1369 - 1378 .

LIM J H , PARK J H , AHN J H , et al . A peptide receptor-based bioelectronic nose for the real-time determination of seafood quality [J ] . Biosensors and Bioelectronics , 2013 , 39 ( 1 ): 244 - 249 .

SMITH R G , D'SOUZA N , NICKLIN S . A review of biosensors and biologically-inspired systems for explosives detection [J ] . Analyst , 2008 , 133 ( 5 ): 571 - 584 .

PAULING L , ROBINSON A B , TERANISHI R , et al . Quantitative analysis of urine vapor and breath by gas-liquid partition chromatography [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1971 , 68 ( 10 ): 2374 - 2376 .

GORDON S M , SZIDON J P , KROTOSZYNSKI B K , et al . Volatile organic compounds in exhaled air from patients with lung cancer [J ] . Clinical Chemistry , 1985 , 31 ( 8 ): 1278 - 1282 .

SHCHERBO D , SHEMIAKINA I I , RYABOVA A V , et al . Near-infrared fluorescent proteins [J ] . Nature Methods , 2010 , 7 ( 10 ): 827 - 829 .

YU D , GUSTAFSON W C , HAN C , et al . An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging [J ] . Nature Communications , 2014 , 5 : 3626 .

MCISAAC R S , ENGQVIST M K M , WANNIER T , et al . Directed evolution of a far-red fluorescent rhodopsin [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2014 , 111 ( 36 ): 13034 - 13039 .

ZHAO L Y , ZOU Q L , YAN X H . Self-assembling peptide-based nanoarchitectonics [J ] . Bulletin of the Chemical Society of Japan , 2019 , 92 ( 1 ): 70 - 79 .

SAWADA T , MIHARA H , SERIZAWA T . Peptides as new smart bionanomaterials: molecular-recognition and self-assembly capabilities [J ] . The Chemical Record , 2013 , 13 ( 2 ): 172 - 186 .

FUKUNAGA K , TSUTSUMI H , MIHARA H . Self-assembling peptides as building blocks of functional materials for biomedical applications [J ] . Bulletin of the Chemical Society of Japan , 2019 , 92 ( 2 ): 391 - 399 .

SERIZAWA T , SAWADA T , MATSUNO H . Highly specific affinities of short peptides against synthetic polymers [J ] . Langmuir , 2007 , 23 ( 22 ): 11127 - 11133 .

MATSUNO H , SEKINE J , YAJIMA H , et al . Biological selection of peptides for poly(L-lactide) substrates [J ] . Langmuir , 2008 , 24 ( 13 ): 6399 - 6403 .

DATE T , SEKINE J , MATSUNO H , et al . Polymer-binding peptides for the noncovalent modification of polymer surfaces: effects of peptide density on the subsequent immobilization of functional proteins [J ] . ACS Applied Materials & Interfaces , 2011 , 3 ( 2 ): 351 - 359 .

SERIZAWA T , FUKUTA H , DATE T , et al . Affinity-based release of polymer-binding peptides from hydrogels with the target segments of peptides [J ] . Chemical Communications , 2016 , 52 ( 11 ): 2241 - 2244 .

ZASLOFF M . Antimicrobial peptides of multicellular organisms [J ] . Nature , 2002 , 415 ( 6870 ): 389 - 395 .

HALE J D , HANCOCK R E . Alternative mechanisms of action of cationic antimicrobial peptides on bacteria [J ] . Expert Review of Anti-Infective Therapy , 2007 , 5 ( 6 ): 951 - 959 .

UZARSKI J R , MELLO C M . Detection and classification of related lipopolysaccharides via a small array of immobilized antimicrobial peptides [J ] . Analytical Chemistry , 2012 , 84 ( 17 ): 7359 - 7366 .

YUAN H X , LIU Z , LIU L B , et al . Cationic conjugated polymers for discrimination of microbial pathogens [J ] . Advanced Materials , 2014 , 26 ( 25 ): 4333 - 4338 .

BORNSCHEUER U T , HUISMAN G W , KAZLAUSKAS R J , et al . Engineering the third wave of biocatalysis [J ] . Nature , 2012 , 485 ( 7397 ): 185 - 194 .

LIN Y H , REN J S , QU X G . Nano-gold as artificial enzymes: hidden talents [J ] . Advanced Materials , 2014 , 26 ( 25 ): 4200 - 4217 .

LIPPINCOTT-SCHWARTZ J , SNAPP E , KENWORTHY A . Studying protein dynamics in living cells [J ] . Nature Reviews Molecular Cell Biology , 2001 , 2 ( 6 ): 444 - 456 .

KANDA T , , SULLIVAN K F , WAHL G M . Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells [J ] . Current Biology , 1998 , 8 ( 7 ): 377 - 385 .

CLARK J , GRZNAROVA P , STANSELL E , et al . A Mason-Pfizer Monkey virus Gag-GFP fusion vector allows visualization of capsid transport in live cells and demonstrates a role for microtubules [J ] . PLoS One , 2013 , 8 ( 12 ): e83863 .

HU S F , CHEN X Y , LEI C Y , et al . Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and tracing [J ] . Chemical Communications , 2018 , 54 ( 56 ): 7806 - 7809 .

DE M , RANA S , AKPINAR H , et al . Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein [J ] . Nature Chemistry , 2009 , 1 ( 6 ): 461 - 465 .

BAJAJ A , RANA S , MIRANDA O R , et al . Cell surface-based differentiation of cell types and cancer states using a gold nanoparticle-GFP based sensing array [J ] . Chemical Science , 2010 , 1 ( 1 ): 134 - 138 .

GENG Y Y , CHATTOPADHYAY A N , ZHANG X Z , et al . Nano assessing nano: nanosensor-enabled detection of cell phenotypic changes identifies nanoparticle toxicological effects at ultra-low exposure levels [J ] . Small , 2020 , 16 ( 36 ): 2002084 .

GENG Y Y , , GOEL H L , LE N B , et al . Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays [J ] . Nanomedicine: Nanotechnology, Biology and Medicine , 2018 , 14 ( 6 ): 1931 - 1939 .

LE N D B , WANG X , GENG Y Y , et al . Rapid and ultrasensitive detection of endocrine disrupting chemicals using a nanosensor-enabled cell-based platform [J ] . Chemical Communications , 2017 , 53 ( 62 ): 8794 - 8797 .

WANG B H , HAN J S , BOJANOWSKI N M , et al . An optimized sensor array identifies all natural amino acids [J ] . ACS Sensors , 2018 , 3 ( 8 ): 1562 - 1568 .

RANA S , ELCI S G , MOUT R , et al . Ratiometric array of conjugated polymers-fluorescent protein provides a robust mammalian cell sensor [J ] . Journal of the American Chemical Society , 2016 , 138 ( 13 ): 4522 - 4529 .

SUZUKI S , SAWADA T , SERIZAWA T . Identification of water-soluble polymers through discrimination of multiple optical signals from a single peptide sensor [J ] . ACS Applied Materials & Interfaces , 2021 , 13 ( 47 ): 55978 - 55987 .

HAN J S , MA C , WANG B H , et al . A hypothesis-free sensor array discriminates whiskies for brand, age, and taste [J ] . Chem , 2017 , 2 ( 6 ): 817 - 824 .

LIU C , YOU X R , LU D K , et al . Gelsolin encountering Ag nanorods/triangles: an aggregation-based colorimetric sensor array for in vivo monitoring the cerebrospinal Aβ 42 % as an indicator of Cd 2+ exposure-related Alzheimer's disease pathogenesis [J ] . ACS Applied Bio Materials , 2020 , 3 ( 11 ): 7965 - 7973 .

MIRANDA O R , CHEN H T , YOU C C , et al . Enzyme-amplified array sensing of proteins in solution and in biofluids [J ] . Journal of the American Chemical Society , 2010 , 132 ( 14 ): 5285 - 5289 .

MIRANDA O R , LI X N , GARCIA-GONZALEZ L , et al . Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor [J ] . Journal of the American Chemical Society , 2011 , 133 ( 25 ): 9650 - 9653 .

HAN J S , CHENG H R , WANG B H , et al . A polymer/peptide complex-based sensor array that discriminates bacteria in urine [J ] . Angewandte Chemie International Edition , 2017 , 56 ( 48 ): 15246 - 15251 .

FAN X B , XU W , HAN J S , et al . Antimicrobial peptide hybrid fluorescent protein based sensor array discriminate ten most frequent clinic isolates [J ] . Biochimica et Biophysica Acta (BBA)-General Subjects , 2019 , 1863 ( 6 ): 1158 - 1166 .

FAN X B , XU W , GAO W , et al . A facile method to classify clinic isolates with a turn-off sensor array based on graphene oxide and antimicrobial peptides [J ] . Sensors and Actuators B: Chemical , 2020 , 307 : 127607 .

FU M Q , WANG X C , DOU W T , et al . Supramolecular fluorogenic peptide sensor array based on graphene oxide for the differential sensing of ebola virus [J ] . Chemical Communications , 2020 , 56 ( 43 ): 5735 - 5738 .

GHANEM E , HOPFER H , NAVARRO A , et al . Predicting the composition of red wine blends using an array of multicomponent peptide-based sensors [J ] . Molecules , 2015 , 20 ( 5 ): 9170 - 9182 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

Weiwei NI

Lingjia ZHOU

Hao WANG

Fei LI

Jinsong HAN

Related Institution

State Key Laboratory of Natural Medicines and National R&D Center for Chinese Herbal Medicine Processing， Department of Food Quality and Safety， College of Engineering， China Pharmaceutical University

⁰