Cell-free multi-enzyme machines for CO<sub>2</sub> capture, utilization and its associated challenges

LIU Jianming; ZENG Anping

doi:10.12211/2096-8280.2022-033

您当前的位置：

首页 >

文章列表页 >

Cell-free multi-enzyme machines for CO₂ capture, utilization and its associated challenges

Comment | 更新时间：2025-03-20

- Cell-free multi-enzyme machines for CO₂ capture, utilization and its associated challenges
- Synthetic Biology Journal Vol. 3, Issue 5, Pages: 825-832(2022)
- 作者机构：
  
  西湖大学合成生物学与生物智造中心，浙江　杭州　310030
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2022-033
  CLC： Q816
- Received：09 June 2022，
  
  Revised：2022-08-11，
  
  Published：31 October 2022
- 稿件说明：
移动端阅览
刘建明，曾安平. 无细胞多酶分子机器赋能二氧化碳的高值利用及其挑战［J］. 合成生物学， 2022， 3（5）： 825-832

LIU Jianming， ZENG Anping. Cell-free multi-enzyme machines for CO₂ capture， utilization and its associated challenges［J］. Synthetic Biology Journal， 2022， 3（5）： 825-832
刘建明，曾安平. 无细胞多酶分子机器赋能二氧化碳的高值利用及其挑战［J］. 合成生物学， 2022， 3（5）： 825-832 DOI： 10.12211/2096-8280.2022-033.

LIU Jianming， ZENG Anping. Cell-free multi-enzyme machines for CO₂ capture， utilization and its associated challenges［J］. Synthetic Biology Journal， 2022， 3（5）： 825-832 DOI： 10.12211/2096-8280.2022-033.

摘要

二氧化碳等温室气体排放导致的全球气候变暖及相应的气候灾难日益严重，开发高效二氧化碳捕获和利用技术迫在眉睫。利用生物制造技术固定二氧化碳是合成生物学工程科学的一个重要攻关方向，其中挖掘和组装无细胞多酶分子机器赋能二氧化碳高值利用，由于背景清晰、调控相对简单、副反应少和产率高等优点，受到越来越多的关注。近期，James C. Liao教授团队人工设计和开发了新型的多酶复合分子机器，建立了用于二氧化碳固定的闭合循环反应-还原型乙醛酸-丙酮酸合成路径（reductive glyoxylate-pyruvate synthesis cycle），可以理论上实现2分子二氧化碳（碳酸氢盐）到1分子乙醛酸的合成反应，并设计和尝试了反应过程中监控辅因子浓度以提高酶稳定性的策略。本文基于设计和组装体外多酶分子机器，聚焦辅因子工程以及利用多酶分子机器固定二氧化碳所面临的挑战等角度讨论这篇工作并简述作者的相关思考。

Abstract

Global warming

mainly caused by the emission of carbo

n dioxide

is becoming a serious problem

and it is urgent to develop efficient carbon dioxide capture and utilization technologies. The use of biomanufacturing technology to fix carbon dioxide is an important research direction in synthetic biology. The mining and

in vitro

assembly of cell-free multi-enzyme machines have the great potential to facilitate the conversion of carbon dioxide into high-value products. The advantages associated with cell-free biosynthesis

such as clear background

relatively simple metabolic regulation

and high-yield production

make it ideal for biomanufacturing. Recently

the team of James C. Liao designed and developed a novel multi-enzyme molecular machine

and established a reductive glyoxylate-pyruvate synthesis cycle

which can theoretically realize the conversion of 2 molecules of carbon dioxide (bicarbonate) to 1 molecule of glyoxylic acid. They also designed and developed a strategy to control the concentration of cofactors during the reaction to improve the enzyme stabilities. In this comment

we discuss this work from the perspectives of

in vitro

multi-enzyme assembly and cofactor engineering

and point to associated challenges of carbon dioxide utilization by multi-enzyme machines.

关键词

Keywords

references

PEPLOW M . The race to upcycle CO 2 into fuels, concrete and more [J ] . Nature , 2022 , 603 ( 7903 ): 780 - 783 .

LIU Z H , WANG K , CHEN Y , et al . Third-generation biorefineries as the means to produce fuels and chemicals from CO 2 [J ] . Nature Catalysis , 2020 , 3 ( 3 ): 274 - 288 .

任杰 , 曾安平 . 基于二氧化碳的生物制造: 从基础研究到工业应用的挑战 [J ] . 合成生物学 , 2021 , 2 ( 6 ): 854 - 862 .

REN Jie , ZENG Anping . CO 2 based biomanufacturing: from basic research to industrial application [J ] . Synthetic Biology Journal , 2021 , 2 ( 6 ): 854 - 862 .

RASOR B J , VÖGELI B , LANDWEHR G M , et al . Toward sustainable, cell-free biomanufacturing [J ] . Current Opinion in Biotechnology , 2021 , 69 : 136 - 144 .

LUO S S , LIN P P , NIEH L Y , et al . A cell-free self-replenishing CO 2 -fixing system [J ] . Nature Catalysis , 2022 , 5 ( 2 ): 154 - 162 .

MAINGUET S E , GRONENBERG L S , WONG S S , et al . A reverse glyoxylate shunt to build a non-native route from C 4 to C 2 in Escherichia coli [J ] . Metabolic Engineering , 2013 , 19 : 116 - 127 .

YU H , LI X Q , DUCHOUD F , et al . Augmenting the Calvin-Benson-Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway [J ] . Nature Communications , 2018 , 9 ( 1 ): 2008 .

HONG Y , REN J , ZHANG X Y , et al . Quantitative analysis of glycine related metabolic pathways for one-carbon synthetic biology [J ] . Current Opinion in Biotechnology , 2020 , 64 : 70 - 78 .

BAR-EVEN A , NOOR E , LEWIS N E , et al . Design and analysis of synthetic carbon fixation pathways [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2010 , 107 ( 19 ): 8889 - 8894 .

SCHWANDER T , VON BORZYSKOWSKI L S , BURGENER S , et al . A synthetic pathway for the fixation of carbon dioxide in vitro [J ] . Science , 2016 , 354 ( 6314 ): 900 - 904 .

XIAO L , LIU G X , GONG F Y , et al . A minimized synthetic carbon fixation cycle [J ] . ACS Catalysis , 2022 , 12 ( 1 ): 799 - 808 .

CAI T , SUN H B , QIAO J , et al . Cell-free chemoenzymatic starch synthesis from carbon dioxide [J ] . Science , 2021 , 373 ( 6562 ): 1523 - 1527 .

ZHENG T T , ZHANG M L , WU L H , et al . Upcycling CO 2 into energy-rich long-chain compounds via electrochemical and metabolic engineering [J ] . Nature Catalysis , 2022 , 5 ( 5 ): 388 - 396 .

MANGAN N M , FLAMHOLZ A , HOOD R D , et al . pH determines the energetic efficiency of the cyanobacterial CO 2 concentrating mechanism [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 36 ): E5354 - E5362 .

王凯 , 刘子鹤 , 陈必强 , 等 . 微生物利用二氧化碳合成燃料及化学品——第三代生物炼制 [J ] . 合成生物学 , 2020 , 1 ( 1 ): 60 - 70 .

WANG K , LIU Z H , CHEN B Q , et al . Microbial utilization of carbon dioxide to synthesize fuels and chemicals — third-generation biorefineries [J ] . Synthetic Biology Journal , 2020 , 1 ( 1 ): 60 - 70 .

GIESSEN T W , SILVER P A . Engineering carbon fixation with artificial protein organelles [J ] . Current Opinion in Biotechnology , 2017 , 46 : 42 - 50 .

YOU C , SHI T , LI Y J , et al . An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 8 ): 1855 - 1864 .

MA W C , XIE S J , ZHANG X G , et al . Promoting electrocatalytic CO 2 reduction to formate via sulfur-boosting water activation on indium surfaces [J ] . Nature Communications , 2019 , 10 : 892 .

GUO W W , LIU S J , TAN X X , et al . Highly efficient CO 2 electroreduction to methanol through atomically dispersed Sn coupled with defective CuO catalysts [J ] . Angewandte Chemie International Edition , 2021 , 60 ( 40 ): 21979 - 21987 .

CLAASSENS N J , COTTON C A R , KOPLJAR D , et al . Making quantitative sense of electromicrobial production [J ] . Nature Catalysis , 2019 , 2 ( 5 ): 437 - 447 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

Jianming LIU

Anping ZENG

Related Institution

Center for Synthetic Biology and Biomanufacturing，Westlake University

⁰

Cell-free multi-enzyme machines for CO2 capture, utilization and its associated challenges

DOI：10.12211/2096-8280.2022-033

摘要

Abstract

关键词

Keywords

references

Cell-free multi-enzyme machines for CO₂ capture, utilization and its associated challenges