Construction and enhancement of enzymatic bioelectrocatalytic systems

CUI Xinyu; WU Ranran; WANG Yuanming; ZHU Zhiguang

doi:10.12211/2096-8280.2022-018

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Construction and enhancement of enzymatic bioelectrocatalytic systems

Invited Review | 更新时间：2025-03-20

- Construction and enhancement of enzymatic bioelectrocatalytic systems
- Synthetic Biology Journal Vol. 3, Issue 5, Pages: 1006-1030(2022)
- 作者机构：
  
  1.中国科学院天津工业生物技术研究所，天津　300308
  2.中国科学院大学，北京　100049
  3.国家合成生物技术创新中心，天津　300308
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2022-018
  CLC： Q819
- Received：01 April 2022，
  
  Revised：2022-06-22，
  
  Published：31 October 2022
- 稿件说明：
移动端阅览
崔馨予，吴冉冉，王园明，朱之光. 酶促生物电催化系统的设计构建与强化［J］. 合成生物学， 2022， 3（5）： 1006-1030

CUI Xinyu， WU Ranran， WANG Yuanming， ZHU Zhiguang. Construction and enhancement of enzymatic bioelectrocatalytic systems［J］. Synthetic Biology Journal， 2022， 3（5）： 1006-1030
崔馨予，吴冉冉，王园明，朱之光. 酶促生物电催化系统的设计构建与强化［J］. 合成生物学， 2022， 3（5）： 1006-1030 DOI： 10.12211/2096-8280.2022-018.

CUI Xinyu， WU Ranran， WANG Yuanming， ZHU Zhiguang. Construction and enhancement of enzymatic bioelectrocatalytic systems［J］. Synthetic Biology Journal， 2022， 3（5）： 1006-1030 DOI： 10.12211/2096-8280.2022-018.

摘要

酶促生物电催化是一种绿色高效的催化技术，充分结合了生物酶催化和电催化的优点，可实现化学能和电能的相互转换，目前已在生物发电、电能存储、CO

固定、传感与监测等方面受到广泛关注。本综述分析了酶促生物电催化的发展现状与当前面临的挑战，从合成生物学的角度详细介绍了氧化还原酶的结构功能和酶促生物电催化系统的基本要素，探讨了酶的改造，包括定向进化、理性设计和引入非天然组件等，以及通过构建多酶复合体模块和强化生物-非生物界面电子传递等方法以提高系统性能。围绕电子传递和能量转化效率等问题，阐述了酶的定向固定方法、电子传递机制以及电极材料设计原则。此外，总结了酶促生物电催化技术在酶燃料电池、生物传感器、化学品酶电合成等合成生物学相关领域的前沿应用。最后，本文展望了未来前景，并提出了从设计改造电活性生物元件、拓宽反

应电势、放大反应系统等方面进一步提升酶促生物电催化系统的性能和可应用性。

Abstract

Enzymatic bioelectrocatalysis

as a green and efficient catalytic technology

combines the advantages of enzymatic catalysis and electrocatalysis to enable the interconversion between chemical energy and electrical energy. It has received extensive attention in the fields of bioelectricity generation

electric energy storage

fixation

biosensing and monitoring

and so on. This review analyzes the recent developments and challenges of enzymatic bioelectrocatalysis. From the perspective of synthetic biology

the structure and function of oxidoreductases which catalyze many biological electron transfer reactions with high speed

selectivity and specificity

and the basic elements of enzymatic bioelectrocatalytic systems are introduced in detail. Strategies of enzyme engineering are discussed

including directed evolution

rational design

and the introduction of non-natural structural components. In addition

the construction of multienzyme complex modules and the enhancement of electron transfer at the biology-abiotic interface

both of which can improve the system performance

are presented. The issues of electron transfer and energy conversion efficiency are further highlighted

and the oriented immobilization of enzymes

electron transfer mechanism

and electrode material modification are discussed. Furthermore

some recent applications of enzymatic bioelectrocatalysis in the frontier fields of synthetic biology are summarized

including enzymatic fuel cells

biosensors

and enzymatic electrosynthesis. Taken together

this review proposes the future directions of engineering bioelectroactive parts

broadening reaction redox potentials

and scaling up reaction systems

in order to further boost the performance of enzymatic bioelectrocatalysis as well as increase its applicability.

关键词

Keywords

references

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