Developing C<sub>1</sub>-based metabolic network in methylotrophy for biotransformation

GUO Shuyuan; WU Lianghuan; LIU Xiangjian; WANG Bo; YU Tao

doi:10.12211/2096-8280.2021-079

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Developing C₁-based metabolic network in methylotrophy for biotransformation

Invited Review | 更新时间：2025-03-20

- Developing C₁-based metabolic network in methylotrophy for biotransformation
- Synthetic Biology Journal Vol. 3, Issue 1, Pages: 116-137(2022)
- 作者机构：
  
  1.中国科学院深圳先进技术研究院，深圳合成生物学创新研究院，合成生物化学研究中心，广东深圳 518055
  2.中国科学院深圳先进技术研究院，深圳合成生物学创新研究院，中国科学院定量工程生物学重点实验室，广东深圳 518055
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2021-079
  CLC： Q 815
- Received：23 July 2021，
  
  Revised：2021-10-21，
  
  Published：28 February 2022
- 稿件说明：
移动端阅览
郭姝媛，吴良焕，刘香健，王博，于涛. 微生物中一碳代谢网络构建的进展与挑战［J］. 合成生物学， 2022， 3（1）： 116-137

GUO Shuyuan， WU Lianghuan， LIU Xiangjian， WANG Bo， YU Tao. Developing C₁-based metabolic network in methylotrophy for biotransformation［J］. Synthetic Biology Journal， 2022， 3（1）： 116-137
郭姝媛，吴良焕，刘香健，王博，于涛. 微生物中一碳代谢网络构建的进展与挑战［J］. 合成生物学， 2022， 3（1）： 116-137 DOI： 10.12211/2096-8280.2021-079.

GUO Shuyuan， WU Lianghuan， LIU Xiangjian， WANG Bo， YU Tao. Developing C₁-based metabolic network in methylotrophy for biotransformation［J］. Synthetic Biology Journal， 2022， 3（1）： 116-137 DOI： 10.12211/2096-8280.2021-079.

摘要

利用来源广、价格低、易制备且储量丰富的一碳化合物作为底物，通过构建甲基营养型细胞工厂，生物合成多种高附加值化学品，不仅可以促进一碳资源的洁净利用，同时可以缓解能源短缺、环境污染等问题。因此，深入了解甲基营养型微生物（天然型和合成型）的一碳代谢网络，是高效利用一碳化合物进行生物炼制的关键。本文综述了多种一碳化合物（甲烷、甲醇、甲酸和二氧化碳）生物炼制的研究进展，主要包括两个部分：（1）甲基营养型微生物（天然型和合成型）的关键代谢酶及多种代谢网络；（2）基于多种甲基营养型微生物进行生物合成的研究现状。文章最后讨论了一碳化合物作为底物进行生物转化所面临的主要瓶颈，并据此提供可行的研究策略，以期推动一碳化合物作为原材料进行生物炼制的工业化进程。

Abstract

One carbon (C

) substrates have generated increasing attention as abundantly available feedstock for biotransformation to produce biofuels and valuable chemicals. Especially

these bioproducts are not only promoting the economic development

but also meet the societal needs for clean energy and environmental protection. Microbial cell factories (MCF) that efficiently convert raw materials to useful chemicals are highly

desirable for C1 based biomanufacturing. Furthermore

more and more attention is being paid to study how the native and synthetic methylotrophic MCFs are capable of utilizing C1 compounds including methane

methanol

formic acid and carbon dioxide (CO

) as raw materials for biosynthesis. Native methylotrophs have multiple pathways for C

utilization so that they can grow with methanol or formate as the sole carbon and energy source. Engineering synthetic methylotrophs are based on available metabolic knowledge and advanced genome engineering tools to modify the platform microorganisms. Hence

it is the key to explore the C

-based metabolic networks of methylotrophy in depth for constructing highly effective methylotrophic cell factory to convert C

substrates. In this review

we firstly summarize in detail key natural pathways for C

-substrate assimilation and bioproducts produced by native methylotrophs. Then we introduce synthetic methylotrophs and major chemical products derived through engineering

Escherichia coli

Corynebacterium glutamicum

and

Saccharomyces cerevisiae

. In the field of synthetic methylotrophs

many studies focus on utilizing methanol or CO

as the sole carbon source and energy source to construct the MCFs for bio-transformation. In the end

we discuss the barriers and challenges for developing robust methylotrophic cell factory to convert C

substrates

and highlight strategies for commercializing the biotransformation of C

based substrates.

关键词

Keywords

references

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Shuyuan Guo

Lianghuan Wu

Xiangjian Liu

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Tao Yu

HUANG Yang

LI Yiye

NIE Guangjun

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Center for Synthetic Biochemistry， Shenzhen Institute of Synthetic Biology， Shenzhen Institutes of Advanced Technology， Chinese Academy of Sciences （CAS）

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety， CAS Center for Excellence in Nanoscience， National Center for Nanoscience and Technology of China

School of Nanoscience and Engineering， University of Chinese Academy of Sciences

College of Materials Science and Opto-Electronic Technology， University of Chinese Academy of Sciences

Department of Bioengineering， College of Chemistry and Chemical Engineering， the North University of China

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Developing C1-based metabolic network in methylotrophy for biotransformation

DOI：10.12211/2096-8280.2021-079

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Developing C₁-based metabolic network in methylotrophy for biotransformation