<italic style="font-style: italic">In vitro</italic> biosynthesis of chemicals: pathway design, component assembly and applications-a review

WAN Yichen; XU Kongliang; ZHENG Renchao; ZHENG Yuguo

doi:10.12211/2096-8280.2021-019

您当前的位置：

首页 >

文章列表页 >

In vitro biosynthesis of chemicals: pathway design, component assembly and applications-a review

Invited Review | 更新时间：2025-03-20

- In vitro biosynthesis of chemicals: pathway design, component assembly and applications-a review
- Synthetic Biology Journal Vol. 2, Issue 6, Pages: 886-901(2021)
- 作者机构：
  
  1.浙江工业大学生物工程学院，生物有机合成技术研究浙江省重点实验室，浙江杭州 310014
  2.浙江工业大学生物工程学院，生物转化与生物净化教育部工程研究中心，浙江杭州 310014
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2021-019
  CLC： Q591.1
- Received：06 February 2021，
  
  Revised：2021-04-30，
  
  Published：31 December 2021
- 稿件说明：
移动端阅览
万逸尘，许孔亮，郑仁朝，郑裕国. 化学品体外生物合成途径设计、元件组装和应用［J］. 合成生物学， 2021， 2（6）： 886-901

WAN Yichen， XU Kongliang， ZHENG Renchao， ZHENG Yuguo. In vitro biosynthesis of chemicals： pathway design， component assembly and applications - a review［J］. Synthetic Biology Journal， 2021， 2（6）： 886-901
万逸尘，许孔亮，郑仁朝，郑裕国. 化学品体外生物合成途径设计、元件组装和应用［J］. 合成生物学， 2021， 2（6）： 886-901 DOI： 10.12211/2096-8280.2021-019.

WAN Yichen， XU Kongliang， ZHENG Renchao， ZHENG Yuguo. In vitro biosynthesis of chemicals： pathway design， component assembly and applications - a review［J］. Synthetic Biology Journal， 2021， 2（6）： 886-901 DOI： 10.12211/2096-8280.2021-019.

摘要

随着合成生物技术的发展，体外生物合成逐渐成为化学品合成的重要方式之一，具有环境友好、催化效率高、原子经济性好、可控性强等优点。途径设计是构建整个体外生物合成系统的关键所在，本文分析总结了体外生物合成中应遵循的两个重要原则，包括原子经济性原则和能量最优原则。利用生物大分子将酶元件组装构建成多酶复合体，可提高体外生物合成的反应速率、减少副反应的发生，本文介绍了用于酶元件组装的3类常见生物大分子，包括连接肽、蛋白支架、DNA等。通过近年来体外生物合成在大宗化学品（糖类化学品、有机酸类化学品以及醇类化学品等）生产中的应用案例的介绍，展示了体外生物合成在化学品合成中的应用前景。随着体外生物合成设计能力的不断提高，体外生物合成的途径设计将朝着智能化、高效化发展，化学品体外生物合成的效率也将逐步提高，有望涵盖所有化学品的生物合成，成为未来化学品合成的主要方式之一。

Abstract

Chemical industry is one of the pillar industries in the modern society. The demand for refined chemicals with high-quality and diversity is rapidly increasing with the improvement of society and human being's living standards. As a supplement to conventional chemical synthesis

environmental friendly biosynthesis is attracting widespread attention and will be an important step to achieve a sustainable development.

In vitro

biosynthesis (cell free biosynthesis) is a synthetic method to prepare desired chemicals

which was catalyzed by purified enzymes or cell extracts. With the development of synthetic biotechnology

in vitro

biosynthesis has gradually become one of the most important ways for chemicals production

exhibiting the advantages of environmental friendliness

high catalytic efficiency

good atom economy and strong controllability. Pathway design is the key for the construction of

in vitro

biosynthesis system. In this review

two important principles for designing

in vitro

biosynthetic pathways have been summarized

including atom economy and energy optimization. As one of the important concepts of green chemistry

principle atom economy means that the synthesis method or process should be designed to convert raw materials into the final product as much as possible. Principle energy optimization means that the ATP-free or ATP minimized process should be designed in the synthesis method. Assembly enzymes into a multi-enzyme complex

via

biological macromolecules can increase reaction rate and also reduce the side reactions of the

in vitro

biosynthesis. Thus

three common-used biological macromolecules for enzyme assembly will be introduced here

including peptide linkers

protein scaffolds

DNA. Some recent examples of chemicals produced

via

in vitro

biosynthesis have also been summarized

including glucosamine

glycerol glucoside

pyruvate

-ketoglutarate

ethanol

3-propanediol

islatravir

azomycin

and

etc

. Through the introduction of

in vitro

biosynthesis of bulk chemicals (carbohydrate chemicals

organic acid chemicals

alcohol chemicals

and etc.)

this article demonstrates the potentials of

in vitro

biosynthesis in chemical synthesis. By reviewing the pathway design

enzyme component assembly and chemicals production examples

the future of

in vitro

biosynthesis of chemicals has been prospected here. With the design improvement of

in vitro

biosynthesis

the pathway designing will be becoming more and more intelligent and efficient. It is believed that the efficiency of

in vitro

biosynth

esis of chemicals will be gradually increased and the

in vitro

biosynthesis hopefully can cover all chemicals' production

which is expected to be one of the predominant ways for chemicals production in the future.

关键词

Keywords

references

LU Y . Biosynthetic inorganic chemistry [J ] . Angewandte Chemie International Edition 2006 , 45 ( 34 ): 5588 - 5601 .

王文豪 , 闻鹏飞 , 许孔亮 , 等 . 工业环境下酶蛋白的催化行为与适应性改造研究进展 [J ] . 生物工程学报 , 2019 , 35 ( 10 ): 1857 - 1869 .

WANG W H , WEN P F , XU K L , et al , Catalysis of enzymes under industrial environment and their adaptive modifications : a review [J ] . Chinese Journal of Biotechnology , 2019 , 35 ( 10 ): 1857 - 1869 .

SHELDON R A , WOODLEY J M . Role of biocatalysis in sustainable chemistry [J ] . Chemical Reviews , 2018 , 118 ( 2 ): 801 - 838 .

SIGRIST R , DA COSTA B Z , MARSAIOLI A J , et al . Nature-inspired enzymatic cascades to build valuable compounds [J ] . Biotechnology Advances , 2015 , 33 ( 5 ): 394 - 411 .

ZHANG Y H . Production of biofuels and biochemicals by in vitro synthetic biosystems: opportunities and challenges [J ] . Biotechnology Advances , 2015 , 33 ( 7 ): 1467 - 1483 .

NYERGES Á , CSÖRGŐ B , NAGY I , et al . A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 9 ): 2502 - 2507 .

LIAN J Z , HAMEDIRAD M , HU S M , et al . Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system [J ] . Nature Communications , 2017 , 8 ( 1 ): 1688 - 1696 .

JESCHEK M , GERNGROSS D , PANKE S . Combinatorial pathway optimization for streamlined metabolic engineering [J ] . Current Opinion in Biotechnology , 2017 , 47 : 142 - 151 .

JESCHEK M , GERNGROSS D , PANKE S . Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort [J ] . Nature Communications , 2016 , 7 : 11163 .

MEADOWS A L , HAWKINS K M , TSEGAYE Y , et al . Rewriting yeast central carbon metabolism for industrial isoprenoid production [J ] . Nature , 2016 , 537 ( 7622 ): 694 - 697 .

ZHANG Y H , SUN J , ZHONG J J . Biofuel production by in vitro synthetic enzymatic pathway biotransformation [J ] . Current Opinion in Biotechnology , 2010 , 21 ( 5 ): 663 - 669 .

KORMAN T P , SAHACHARTSIRI B , LI D , et al . A synthetic biochemistry system for the in vitro production of isoprene from glycolysis intermediates [J ] . Protein Science , 2014 , 23 ( 5 ): 576 - 585 .

YE X T , HONDA K , SAKAI T , et al . Synthetic metabolic engineering - a novel, simple technology for designing a chimeric metabolic pathway [J ] . Microbial Cell Factories , 2012 , 11 : 120 .

MENG D D , WEI X L , BAI X , et al . Artificial in vitro synthetic enzymatic biosystem for the one-pot sustainable biomanufacturing of glucosamine from starch and inorganic ammonia [J ] . ACS Catalysis , 2020 , 10 ( 23 ): 13809 - 13819 .

BEER B , PICK A , SIEBER V . In vitro metabolic engineering for the production of α -ketoglutarate [J ] . Metabolic Engineering , 2017 , 40 : 5 - 13 .

GUTERL J K , GARBE D , CARSTEN J , et al . Cell-free metabolic engineering: production of chemicals by minimized reaction cascades [J ] . ChemSusChem , 2012 , 5 ( 11 ): 2165 - 2172 .

ZHANG T G , YANG J G , TIAN C Y , et al . High-yield biosynthesis of glucosylglycerol through coupling phosphorolysis and transglycosylation reactions [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 51 ): 15249 - 15256 .

HONDA K , KIMURA K , NINH P H , et al . In vitro bioconversion of chitin to pyruvate with thermophilic enzymes [J ] . Journal of Bioscience and Bioengineering , 2017 , 124 / 125 /126/127/128/ 129 ( 3 ): 296 - 301 .

RIECKENBERG F , ARDAO I , RUJANANON R , et al . Cell-free synthesis of 1,3-propanediol from glycerol with a high yield [J ] . Engineering in Life Sciences , 2014 , 14 ( 4 ): 380 - 386 .

HUFFMAN M A , FRYSZKOWSKA A , ALVIZO O . Design of an in vitro biocatalytic cascade for the manufacture of islatravir [J ] . Science , 2019 , 366 ( 6470 ): 1255 - 1259 .

HEDGES J B , RYAN K S . In vitro reconstitution of the biosynthetic pathway to the nitroimidazole antibiotic azomycin [J ] . Angewandte Chemie International Edition , 2019 , 58 ( 34 ): 11647 - 11651 .

MORADIAN A , BENNER S A . A biomimetic biotechnological process for converting starch to fructose: thermodynamic and evolutionary considerations in applied enzymology [J ] . Journal of the American Chemical Society , 1992 , 114 ( 18 ): 6980 - 6987 .

KRUTSAKORN B , HONDA K , YE X T , et al . In vitro production of n -butanol from glucose [J ] . Metabolic Engineering , 2013 , 20 : 84 - 91 .

RUALES-SALCEDO A V , HIGUITA J C , FONTALVO J , et al . Design of enzymatic cascade processes for the production of low-priced chemicals [J ] . Zeitschrift fur Naturforschung C , Journal of Biosciences, 2019 , 74 ( 3/4 ): 77 - 84 .

DUDLEY Q M , KARIM A S , JEWETT M C . Cell-free metabolic engineering: biomanufacturing beyond the cell [J ] . Biotechnology Journal , 2015 , 10 ( 1 ): 69 - 82 .

ZANG Y , ZHA J , WU X , et al . In vitro naringenin biosynthesis from p -coumaric acid using recombinant enzymes [J ] . Journal of Agricultural and Food Chemistry , 2019 , 67 ( 49 ): 13430 - 13436 .

MINAMI A , SHIMAYA M , SUZUKI G , et al . Sequential enzymatic epoxidation involved in polyether lasalocid biosynthesis [J ] . Journal of the American Chemical Society , 2012 , 134 ( 17 ): 7246 - 7249 .

HANATANI Y , IMURA M , TANIGUCHI H , et al . In vitro production of cysteine from glucose [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 19 ): 8009 - 8019 .

COREY E J . The logic of chemical synthesis-multisteps synthesis of complex carbogenic molecules [J ] . Angewandte Chemie International Edition , 1991 , 30 ( 5 ): 455 - 465 .

CARBONELL P , PLANSON A G , FAULON J L . Retrosynthetic design of heterologous pathways [M ] // ALPER H S. Systems Metabolic Engineering . Springer , 2013 : 149 - 173 .

BIRMINGHAM W R , STARBIRD C A , PANOSIAN T D , et al . Bioretrosynthetic construction of a didanosine biosynthetic pathway [J ] . Nature Chemical Biology , 2014 , 10 ( 5 ): 392 - 399 .

MINAMI A , MIGITA A , INADA D , et al . Enzymatic epoxide-opening cascades catalyzed by a pair of epoxide hydrolases in the ionophore polyether biosynthesis [J ] . Organic Letters , 2011 , 13 ( 7 ): 1638 - 1641 .

MENG D D , LIANG A L , WEI X L , et al . Enzymatic characterization of a thermostable phosphatase from Thermomicrobium roseum and its application for biosynthesis of fructose from maltodextrin [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 15 ): 6129 - 6139 .

HWANG E T , LEE S . Multienzymatic cascade reactions via enzyme complex by immobilization [J ] . ACS Catalysis , 2019 , 9 ( 5 ): 4402 - 4425 .

贺俊斌 , 孟松 , 潘海学 , 等 . 多酶催化串联策略在复杂天然产物合成中的应用 [J ] . 合成生物学 , 2020 , 1 ( 2 ): 226 - 246 .

HE J B , MENG S , PAN H X , et al . Applications of the multienzyme-catalyzed tandem strategy in the synthesis of complex natural products [J ] . Synthetic Biology Journal , 2020 , 1 ( 2 ): 226 - 246 .

WANG W , YANG J G , SUN Y X , et al ., Artificial ATP-free in vitro synthetic enzymatic biosystems facilitate aldolase-mediated C-C bond formation for biomanufacturing [J ] . ACS Catalysis , 2020 , 10 ( 2 ): 1264 - 1271 .

MORDHORST S , ANDEXER J N . Round, round we go - strategies for enzymatic cofactor regeneration [J ] . Natural Product Reports , 2020 , 37 ( 10 ): 1316 - 1333 .

WANG Y R , ZHANG Y H P , Cell-free protein synthesis energized by slowly-metabolized maltodextrin [J ] . BMC Biotechnology , 2009 , 9 : 58 - 65 .

ZHANG X , WU H , HUANG B , et al . One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system [J ] . Biotechnology Journal , 2017 , 241 : 163 - 169 .

MORDHORST S , SINGH J , MOHR M K F , et al . Several polyphosphate kinase 2 enzymes catalyse the production of adenosine 5 ' -polyphosphates [J ] . Chembiochem , 2019 , 20 ( 8 ): 1019 - 1022 .

SIEBERS B , HENSEL R . Glucose catabolism of the hyperthermophilic archaeum thermoproteus-tenax [J ] . Fems Microbiology Letters , 1993 , 111 ( 1 ): 1 - 8 .

OKANO K , ZHU Q , HONDA K . In vitro reconstitution of non-phosphorylative Entner-Doudoroff pathway for lactate production [J ] . Journal of Bioscience and Bioengineering , 2020 , 129 ( 3 ): 269 - 275 .

MARSH J A , TEICHMANN S A , Structure , dynamics , assembly , and evolution of protein complexes [J ] . Annual Review of Biochemistry , 2015 , 84 , 551 - 575 .

SCHOFFELEN S , VAN HEST J C , Chemical approaches for the construction of multi-enzyme reaction systems [J ] . Current Opinion in Structural Biology , 2013 , 23 ( 4 ), 613 - 621 .

WANG S Z , ZHANG Y H , REN H , et al . Strategies and perspectives of assembling multi-enzyme systems [J ] . Critical Reviews in Biotechnology , 2017 , 37 ( 8 ): 1024 - 1037 .

HAGA T , HIRAKAWA H , NAGAMUNE T . Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker [J ] . PLoS One , 2013 , 8 ( 9 ): e75114 .

ITURRATE L , SáNCHEZ-MORENO I , DOYAGÜEZ E G , et al . Substrate channelling in an engineered bifunctional aldolase/kinase enzyme confers catalytic advantage for C-C bond formation [J ] . Chemical Communications , 2009 ( 13 ): 1721 - 1723 .

HUANG Z , YE F , ZHANG C , et al . Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection [J ] . Journal of Biotechnology , 2013 , 163 ( 1 ): 30 - 37 .

FAN L W , WANG Y , TUYISHIME P , et al . Engineering artificial fusion proteins for enhanced methanol bioconversion [J ] . Chembiochem , 2018 , 19 ( 23 ): 2465 - 2471 .

LERCHNER A , DAAKE M , JARASCH A , et al . Fusion of an alcohol dehydrogenase with an aminotransferase using a PAS linker to improve coupled enzymatic alcohol-to-amine conversion [J ] . Protein Engineering, Design and Selection , 2016 , 29 ( 12 ): 557 - 562 .

魏欣蕾 , 游淳 . 体外多酶分子机器的现状和最新进展 [J ] . 生物工程学报 , 2019 , 35 ( 10 ): 1870 - 1888 .

WEI X L , YOU C . In vitro multi-enzyme molecular machines-a review [J ] . Chinese Journal of Biotechnology , 2019 , 35 ( 10 ): 1870 - 1888 .

VAZANA Y , BARAK Y , UNGER T , et al . A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates [J ] . Biotechnology for Biofuels , 2013 , 6 ( 1 ): 182 .

ANANDHARAJ M , LIN Y J , RANI R P , et al . Constructing a yeast to express the largest cellulosome complex on the cell surface [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2020 , 117 ( 5 ): 2385 - 2394 .

YOU C , ZHANG Y H P . Self-assembly of synthetic metabolons through synthetic protein scaffolds: one-step purification, co-immobilization, and substrate channeling [J ] . ACS Synthetic Biology , 2013 , 2 ( 2 ): 102 - 110 .

JEONG D W , HYEON J E , SHIN S K , et al . Trienzymatic complex system for isomerization of agar-derived D-galactose into D-tagatose as a low-calorie sweetener [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 10 ): 3195 - 3202 .

SHIMADA J , MARUYAMA T , KITAOKA M , et al . Programmable protein-protein conjugation via DNA-based self-assembly [J ] . Chemical Communications , 2012 , 48 ( 50 ): 6226 - 6228 .

SONG J Y , HE W T , SHEN H , et al . Self-assembly of a magnetic DNA hydrogel as a new biomaterial for enzyme encapsulation with enhanced activity and stability [J ] . Chemical Communications , 2019 , 55 ( 17 ): 2449 - 2452 .

SIMMEL F C . DNA-based assembly lines and nanofactories [J ] . Current Opinion in Biotechnology , 2012 , 23 ( 4 ): 516 - 521 .

XU K L , CHEN X X , ZHENG R C , et al . Immobilization of multi-enzymes on support materials for efficient biocatalysis [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 660 .

XIN L , ZHOU C , YANG Z Q , et al . Regulation of an enzyme cascade reaction by a DNA machine [J ] . Small , 2013 , 9 ( 18 ): 3088 - 3091 .

FU J L , YANG Y H R , JOHNSON-BUCK A , et al . Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm [J ] . Nature Nanotechnology , 2014 , 9 ( 7 ): 531 - 536 .

MÜLLER J , NIEMEYER C M . DNA-directed assembly of artificial multienzyme complexes [J ] . Biochemical and Biophysical Research Communications , 2008 , 377 ( 1 ): 62 - 67 .

MA Q Y , GAO X Z . Categories and biomanufacturing methods of glucosamine [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 19 ): 7883 - 7889 .

JIANG Z , LÜ X Q , LIU Y F , et al . Biocatalytic production of glucosamine from N -acetylglucosamine by diacetylchitobiose deacetylase [J ] . Journal of Microbiology and Biotechnology , 2018 , 28 ( 11 ): 1850 - 1858 .

LV Y M , LABORDA P , HUANG K , et al . Highly efficient and selective biocatalytic production of glucosamine from chitin [J ] . Green Chemistry , 2017 , 19 ( 2 ): 527 - 535 .

SU C , ALLUM A J , AIZAWA Y , et al . Novel glyceryl glucoside is a low toxic alternative for cryopreservation agent [J ] . Biochemical and Biophysical Research Communications , 2016 , 476 ( 4 ): 359 - 364 .

TAKENAKA F , UCHIYAMA H . Effects of α -D-glucosylglycerol on the in vitro digestion of disaccharides by rat intestinal enzymes [J ] . Bioscience, Biotechnology, and Biochemistry , 2001 , 65 ( 7 ): 1458 - 1463 .

KRUTSAKORN B , IMAGAWA T , HONDA K , et al . Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N- acetylglutamate production [J ] . Microbial Cell Factories , 2013 , 12 : 91 .

YOU C , MYUNG S , ZHANG Y H P . Facilitated substrate channeling in a self-assembled trifunctional enzyme complex [J ] . Angewandte Chemie International Edition , 2012 , 51 ( 35 ): 8787 - 8790 .

WANG W , LIU M X , YOU C , et al . ATP-free biosynthesis of a high-energy phosphate metabolite fructose 1,6-diphosphate by in vitro metabolic engineering [J ] . Metabolic Engineering , 2017 , 42 : 168 - 174 .

HONDA K , MAYA S , OMASA T , et al . Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes [J ] . Journal of Biotechnology , 2010 , 148 ( 4 ): 204 - 207 .

GRIMAUD F , PIZZUT-SERIN S , TARQUIS L , et al . In vitro synthesis and crystallization of β -1,4-mannan [J ] . Biomacromolecules , 2019 , 20 ( 2 ): 846 - 853 .

TIAN C Y , YANG J G , LI Y J , et al . Artificially designed routes for the conversion of starch to value-added mannosyl compounds through coupling in vitro and in vivo metabolic engineering strategies [J ] . Metabolic Engineering , 2020 , 61 : 215 - 224 .

KIM J E , ZHANG Y H P . Biosynthesis of D-xylulose 5-phosphate from D-xylose and polyphosphate through a minimized two-enzyme cascade [J ] . Biotechnology and Bioengineering , 2016 , 113 ( 2 ): 275 - 282 .

HUANG H J , LIU L M , LI Y , et al . Redirecting carbon flux in Torulopsis glabrata from pyruvate to α -ketoglutaric acid by changing metabolic co-factors [J ] . Biotechnology Letters , 2006 , 28 ( 2 ): 95 - 98 .

STOTTMEISTER U , AURICH A , WILDE H , et al . White biotechnology for green chemistry: fermentative 2-oxocarboxylic acids as novel building blocks for subsequent chemical syntheses [J ] . Journal of Industrial Microbiology and Biotechnology , 2005 , 32 ( 11/12 ): 651 - 664 .

GUO H W , MADZAK C , DU G C , et al . Effects of pyruvate dehydrogenase subunits overexpression on the α -ketoglutarate production in Yarrowia lipolytica WSH-Z06 [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 16 ): 7003 - 7012 .

CHEN X , CUI Y F , CHENG X K , et al . Highly atom economic synthesis of D-2-aminobutyric acid through an in vitro tri-enzymatic catalytic system [J ] . ChemistryOpen , 2017 , 6 ( 4 ): 534 - 540 .

SU H H , GUO Z W , WU X L , et al ., Efficient bioconversion of sucrose to high-value-added glucaric acid by in vitro metabolic engineering [J ] . ChemSusChem , 2019 , 12 ( 10 ): 2278 - 2285 .

ATSUMI S , HANAI T , LIAO J C . Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels [J ] . Nature , 2008 , 451 ( 7174 ): 86 - 89 .

GALVAGNO M A , IANNONE L J , BIANCHI J , et al . Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol [J ] . Revista Argentina de Microbiologia , 2011 , 43 ( 3 ): 218 - 225 .

AHRENS K , MENZEL K , ZENG A , et al . Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture (Ⅲ): Enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation [J ] . Biotechnology and Bioengineering , 1998 , 59 ( 5 ): 544 - 552 .

HAN P P , ZHOU X G , YOU C . Efficient multi-enzymes immobilized on porous microspheres for producing inositol from starch [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 380 .

FUJISAWA T , FUJINAGA S , ATOMI H , An in vitro enzyme system for the production of myo-inositol from starch [J ] . Applied and Environmental Microbiology , 2017 , 83 ( 16 ): e00550-17 .

ZHANG R B , LIU W , CAO Y J , et al . An in vitro synthetic biosystem based on acetate for production of phloroglucinol [J ] . BMC Biotechnology , 2017 , 17 ( 1 ): 66 .

LI Z L , NING X , ZHAO Y R , et al . Efficient one-pot synthesis of cytidine 5 ' -monophosphate using an extremophilic enzyme cascade system [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 34 ): 9188 - 9194 .

VALENCIA L E , ZHANG Z C , CEPEDA A J , et al . Seven-enzyme in vitro cascade to (3 R )-3-hydroxybutyryl-CoA [J ] . Organic & Biomolecular Chemistry , 2019 , 17 ( 6 ): 1375 - 1378 .

KORMAN T P , OPGENORTH P H , BOWIE J U . A synthetic biochemistry platform for cell free production of monoterpenes from glucose [J ] . Nature Communications , 2017 , 8 : 15526 .

BARRETT S E , TELLER R S , FORSTER S P , et al . Extended-duration MK-8591-eluting implant as a candidate for HIV treatment and prevention [J ] . Antimicrobial Agents and Chemotherapy , 2018 , 62 ( 10 ): 18 - 30 .

MCLAUGHLIN M , KONG J , BELYK K M , et al . Enantioselective synthesis of 4 ' -ethynyl-2-fluoro-2 ' -deoxyadenosine (EFdA) via enzymatic desymmetrization [J ] . Organic Letters , 2017 , 19 ( 4 ): 926 - 929 .

NAKANE A , NAKAMURA T , EGUCHI Y . A novel metabolic fate of arginine in Streptomyces eurocidicus . Partial resolution of the pathway and identification of an intermediate [J ] . The Journal of Biological Chemistry , 1977 , 252 ( 15 ): 5267 - 5273 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Applications of protein engineering in pharmaceutical industry

A review on enzyme-catalyzed synthesis of chiral amino acids

Research progress of artificial intelligence in desiging protein structures

Recent development of directed evolution in protein engineering

Related Author

Yuguo ZHENG

Renchao ZHENG

Kongliang XU

Yichen WAN

WEN Yanhua

LIU Hedong

CAO Chunlai

WU Ruibo

Related Institution

Key Laboratory of Bioorganic Synthesis of Zhejiang Province， College of Biotechnology and Bioengineering， Zhejiang University of Technology

Engineering Research Center of Bioconversion and Biopurification of Ministry of Education，Zhejiang University of Technology

The United Biotechnology （Zhuhai Hengqin） Co.， Ltd.

Zhuhai United Laboratories Co.， Ltd

School of Pharmaceutical Science，Sun Yat-Sen University

⁰

In vitro biosynthesis of chemicals: pathway design, component assembly and applications-a review

DOI：10.12211/2096-8280.2021-019

摘要

Abstract

关键词

Keywords

references