- Editorial Board
- Journal
- Preprinting Policy
- 个人中心
- 退出登录
浏览全部资源
扫码关注微信
- Editorial Board
- The 2nd EB
- The 1st EB
- Journal
- Latest Issue
- Archive
- Online First
- Preprinting Policy
- Preprinting Policy
- Data Policy
Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology
- Synthetic Biology Journal Vol. 2, Issue 6, Pages: 863-875(2021)
- 作者机构：
1.江南大学糖化学与生物技术教育部重点实验室，江苏 无锡 214122
2.江南大学未来食品科学中心，江苏 无锡 214122
3.江南大学工业生物技术教育部重点实验室，江苏 无锡 214122
- 作者简介：
- 基金信息：
DOI：10.12211/2096-8280.2021-015
CLC： Q815Received：02 February 2021，
Revised：2021-08-09，
Published：31 December 2021
- 稿件说明：
移动端阅览
张晓龙， 王晨芸， 刘延峰， 李江华， 刘龙， 堵国成. 基于合成生物技术构建高效生物制造系统的研究进展［J］. 合成生物学， 2021， 2（6）： 863-875
ZHANG Xiaolong， WANG Chenyun， LIU Yanfeng， LI Jianghua， LIU Long， DU Guocheng. Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology［J］. Synthetic Biology Journal， 2021， 2（6）： 863-875
张晓龙， 王晨芸， 刘延峰， 李江华， 刘龙， 堵国成. 基于合成生物技术构建高效生物制造系统的研究进展［J］. 合成生物学， 2021， 2（6）： 863-875 DOI： 10.12211/2096-8280.2021-015.
ZHANG Xiaolong， WANG Chenyun， LIU Yanfeng， LI Jianghua， LIU Long， DU Guocheng. Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology［J］. Synthetic Biology Journal， 2021， 2（6）： 863-875 DOI： 10.12211/2096-8280.2021-015.
摘要
基于合成生物技术构建绿色高效的生物制造系统是实现可持续化发展的重要途径，该技术的发展应用有望为食品、能源、医药、化工以及畜牧养殖等行业带来革命性的技术变革。本文针对基于合成生物技术构建高效生物制造系统进行系统性的总结与讨论。首先概述了代谢工程、酶工程、辅助系统优化以及发酵过程控制等技术的研究进展；其次，着重对比总结了大肠杆菌、芽孢杆菌属、谷氨棒酸杆菌以及酵母属等典型模式宿主的代谢特性，探究了各微生物制造系统的适用范围。最后，对合成生物技术在构建高效生物制造系统领域中的应用前景进行了展望。精细多元的代谢工程技术、高效简便的酶工程策略以及数字化的微生物系统将是促进高效生物制造系统构建的新引擎与新动力。
Abstract
Efficient and environmentally friendly biomanufacturing system based on synthetic biotechnology is an important approach to achieve sustainable development. Synthetic biology is expected to bring revolutionary technological breakthroughs in various industries
such as food
pharmacy and chemistry
as well as farming and animal husbandry. In this paper
the latest advances of technologies and strategies in synthetic biology used in the progress of constructing efficient biomanufacturing systems were introduced. Four aspects
namely metabolic regulation of key genes
enzyme engineering
cofactor engineering and fermentation optimization
were discussed. Through these technologies
engineered microorganisms with high robustness and excellent performance were constructed. Secondly
an emphasis was put on the summary of diverse metabolic characteristics of typical model organisms at present. In this part
as many as seven strains were mentioned
such as
Escherichia coli
Bacillus subtilis
Bacillus amyloliquefaciens
Bacillus licheniformis
Corynebacterium glutamicum
Pichia pastoris
and
Saccharomyces cerevisiae
. And for each strain
the advantages and disadvantages of different typical model organisms were discussed to clarify the scope of their most suitable products.
Escherichia coli
is the most intensively studied typical model organism system
making it the preferred expression system for proteins of interest. However
insufficient post-translational processing limits its applications for expressing eukaryotic-derived proteins.
Saccharomyces cerevisiae
and
Pichia pastoris
make up for this deficiency. Yeast expression system has significant advantages for the synthesis of natural products from plant
due to the extensive and in-depth research of P450 enzymes
such as the biosynthesis of artemisinin. Lastly
application prospects of synthetic biology in constructing efficient biomanufacturing systems were discussed. With the developments in standard synthetic biology components and data
standard automated work platforms
precise and generally applicable engineering strategies
emerging of machine learning and synthetic biology
it is expected to facilitate efficient biological manufacturing system construction. Precise and various metabolic engineering technology
flexible and convenient enzyme engineering strategies and whole cell microorganism modeling would be the new driving force for efficient biomanufacturing system construction.
2
关键词
Keywords
references
SHEN X L , WANG J , LI C Y , et al . Dynamic gene expression engineering as a tool in pathway engineering [J ] . Current Opinion in Biotechnology , 2019 , 59 : 122 - 129 .
WU Y K , CHEN T C , LIU Y F , et al . Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis [J ] . Nucleic Acids Research , 2020 , 48 ( 2 ): 996 - 1009 .
SANDER T , FARKE N , DIEHL C , et al . Allosteric feedback inhibition enables robust amino acid biosynthesis in E. coli by enforcing enzyme overabundance [J ] . Cell Systems , 2019 , 8 ( 1 ): 66 - 75.e8 .
陈修来 , 刘佳 , 罗秋玲 , 等 . 微生物辅因子平衡的代谢调控 [J ] . 生物工程学报 , 2017 , 33 ( 1 ): 16 - 26 .
CHEN X L , LIU J , LUO Q L , et al . Manipulation of cofactor balance in microorganisms [J ] . Chinese Journal of Biotechnology , 2017 , 33 ( 1 ): 16 - 26 .
GU Y , LÜ X Q , LIU Y F , et al . Synthetic redesign of central carbon and redox metabolism for high yield production of N -acetylglucosamine in Bacillus subtilis [J ] . Metabolic Engineering , 2019 , 51 : 59 - 69 .
KIM Y E , HIPP M S , BRACHER A , et al . Molecular chaperone functions in protein folding and proteostasis [J ] . Annual Review of Biochemistry , 2013 , 82 : 323 - 355 .
LIANG C N , ZHANG X X , WU J Y , et al . Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit [J ] . Metabolic Engineering , 2020 , 57 : 239 - 246 .
POLKA J K , HAYS S G , SILVER P A . Building spatial synthetic biology with compartments, scaffolds, and communities [J ] . Cold Spring Harbor Perspectives in Biology , 2016 , 8 ( 8 ): a024018 .
WANG Y , HU L T , HUANG H , et al . Eliminating the capsule-like layer to promote glucose uptake for hyaluronan production by engineered Corynebacterium glutamicum [J ] . Nature Communications , 2020 , 11 ( 1 ): 3120 .
陈坚 , 刘立明 , 堵国成 . 发酵过程优化原理与技术 [M ] . 北京 : 化学工业出版社 , 2009 : 5 - 14 .
CHEN J , LIU L M , DU G C . Principle and technology of fermentation optimization [M ] . Beijing : Chemical Industry Press , 2009 : 5 - 14 .
GOPAL G J , KUMAR A . Strategies for the production of recombinant protein in Escherichia coli [J ] . The Protein Journal , 2013 , 32 ( 6 ): 419 - 425 .
TERPE K . Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems [J ] . Applied Microbiology and Biotechnology , 2006 , 72 ( 2 ): 211 - 222 .
TERPE K . Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems [J ] . Applied Microbiology and Biotechnology , 2003 , 60 ( 5 ): 523 - 533 .
ROSANO G L , CECCARELLI E A . Recombinant protein expression in Escherichia coli : advances and challenges [J ] . Frontiers in Microbiology , 2014 , 5 : 172 .
SCHLEIF R . AraC protein, regulation of the L-arabinose operon in Escherichia coli , and the light switch mechanism of AraC action [J ] . FEMS Microbiology Reviews , 2010 , 34 ( 5 ): 779 - 796 .
QING G L , MA L C , KHORCHID A , et al . Cold-shock induced high-yie ld protein production in Escherichia coli [J ] . Nature Biotechnology , 2004 , 22 ( 7 ): 877 - 882 .
WANG X , HAN J N , ZHANG X , et al . Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli [J ] . Nature Communications , 2021 , 12 ( 1 ): 1411 .
COSTA S J , ALMEIDA A , CASTRO A , et al . The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli : a comparison with the traditional gene fusion technology [J ] . Applied Microbiology and Biotechnology , 2013 , 97 ( 15 ): 6779 - 6791 .
BANKI M R , FENG L , WOOD D W . Simple bioseparations using self-cleaving elastin-like polypeptide tags [J ] . Nature Methods , 2005 , 2 ( 9 ): 659 - 661 .
KAPUST R B , TÖZSÉR J , COPELAND T D , et al . The P1′ specificity of tobacco etch virus protease [J ] . Biochemical and Biophysical Research Communications , 2002 , 294 ( 5 ): 949 - 955 .
CHOI J H , LEE S Y . Secretory and extracellular production of recombinant proteins using Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2004 , 64 ( 5 ): 625 - 635 .
SCHIERLE C F , BERKMEN M , HUBER D , et al . The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway [J ] . Journal of Bacteriology , 2003 , 185 ( 19 ): 5706 - 5713 .
SOARES C R J , GOMIDE F I C , UEDA E K M , et al . Periplasmic expression of human growth hormone via plasmid vectors containing the λPL promoter: use of HPLC for product quantification [J ] . Protein Engineering, Design and Selection , 2003 , 16 ( 12 ): 1131 - 1138 .
MESSENS J , COLLET J F . Pathways of disulfide bond formation in Escherichia coli [J ] . The International Journal of Biochemistry & Cell Biology , 2006 , 38 ( 7 ): 1050 - 1062 .
NISHIHARA K , KANEMORI M , YANAGI H , et al . Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli [J ] . Applied and Environmental Microbiology , 2000 , 66 ( 3 ): 884 - 889 .
FERRER M , LÜNSDORF H , CHERNIKOVA T N , et al . Functional consequences of single: double ring transitions in chaperonins: life in the cold [J ] . Molecular Microbiology , 2004 , 53 ( 1 ): 167 - 182 .
LUEKING A , HOLZ C , GOTTHOLD C , et al . A system for dual protein expression in Pichia pastoris and Escherichia coli [J ] . Protein Expression and Purification , 2000 , 20 ( 3 ): 372 - 378 .
STEWART E J , ÅSLUND F , BECKWITH J . Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins [J ] . The EMBO Journal , 1998 , 17 ( 19 ): 5543 - 5550 .
KIM K , CHOE D , LEE D-H , et al . Engineering biology to construct microbial chassis for the production of difficult-to-express proteins [J ] . International Journal of Molecular Sciences , 2020 , 21 ( 3 ): 990 .
FAULKNER M J , VEERAVALLI K , GON S , et al . Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2008 , 105 ( 18 ): 6735 - 6740 .
ZELIĆ B , GOSTOVIĆ S , VUORILEHTO K , et al . Process strategies to enhance pyruvate production with recombinant Escherichia coli : from repetitive fed-batch to in situ product recovery with fully integrated electrodialysis [J ] . Biotechnology and Bioengineering , 2004 , 85 ( 6 ): 638 - 646 .
YANG M H , ZHANG X . Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis [J ] . World Journal of Microbiology and Biotechnology , 2017 , 33 ( 3 ): 59 .
MATSUMOTO T , TANAKA T , KONDO A . Engineering metabolic pathways in Escherichia coli for constructing a "microbial chassis" for biochemical production [J ] . Bioresource Technology , 2017 , 245 : 1362 - 1368 .
KRIVORUCHKO A , ZHANG Y M , SIEWERS V , et al . Microbial acetyl-CoA metabolism and metabolic engineering [J ] . Metabolic Engineering , 2015 , 28 : 28 - 42 .
HUANG J F , LIU Z Q , JIN L Q , et al . Metabolic engineering of Escherichia coli for microbial production of L-methionine [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 4 ): 843 - 851 .
YANG P , WANG J , PANG Q X , et al . Pathway optimization and key enzyme evolution of N -acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor [J ] . Metabolic Engineering , 2017 , 43 ( A ): 21 - 28 .
YE L J , ZHANG C Z , BI C H , et al . Combinatory optimization of chromosomal integrated mevalonate pathway for β -carotene production in Escherichia coli [J ] . Microbial Cell Factories , 2016 , 15 ( 1 ): 202 .
ALONSO-GUTIERREZ J , CHAN R , BATTH T S , et al . Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production [J ] . Metabolic Engineering , 2013 , 19 : 33 - 41 .
ZHOU L , DING Q , JIANG G Z , et al . Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A [J ] . Microbial Cell Factories , 2017 , 16 ( 1 ): 84 .
SCHALLMEY M , SINGH A , WARD O P . Developments in the use of Bacillus species for industrial production [J ] . Canadian Journal of Microbiology , 2004 , 50 ( 1 ): 1 - 17 .
PETSCH D , ANSPACH F B . Endotoxin removal from protein solutions [J ] . Journal of Biotechnology , 2000 , 76 ( 2/3 ): 97 - 119 .
SHI T , WANG Y C , WANG Z W , et al . Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis [J ] . Microbial Cell Factories , 2014 , 13 ( 1 ): 101 .
JIN P , KANG Z , YUAN P H , et al . Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168 [J ] . Metabolic Engineering , 2016 , 35 : 21 - 30 .
GU Y , XU X H , WU Y K , et al . Advances and prospects of Bacillus subtilis cellular factories: from rational design to industrial applications [J ] . Metabolic Engineering , 2018 , 50 : 109 - 121 .
SAITO N . A thermophilic extracellular α -amylase from Bacillus licheniformis [J ] . Archives of Biochemistry and Biophysics , 1973 , 155 ( 2 ): 290 - 298 .
MACHIUS M , DECLERCK N , HUBER R , et al . Kinetic stabilization of Bacillus licheniformis α -amylase through introduction of hydrophobic residues at the surface [J ] . Journal of Biological Chemistry , 2003 , 278 ( 13 ): 11546 - 11553 .
SELLAMI-KAMOUN A , HADDAR A , ALI N E-H , et al . Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid laundry detergent formulatio ns [J ] . Microbiological Research , 2008 , 163 ( 3 ): 299 - 306 .
KALIMUTHU K , BABU R S , VENKATARAMAN D , et al . Biosynthesis of silver nanocrystals by Bacillus licheniformis [J ] . Colloids and Surfaces B: Biointerfaces , 2008 , 65 ( 1 ): 150 - 153 .
KALISHWARALAL K , DEEPAK V , RAMKUMARPANDIAN S , et al . Extracellular biosynthesis of silver nanoparticles by the culture supernatant of Bacillus licheniformis [J ] . Materials Letters , 2008 , 62 ( 29 ): 4411 - 4413 .
SHIH I L , VAN Y T , YEH L C , et al . Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties [J ] . Bioresource Technology , 2001 , 78 ( 3 ): 267 - 272 .
CAO M F , FENG J , SIRISANSANEEYAKUL S , et al . Genetic and metabolic engineering for microbial production of poly- γ -glutamic acid [J ] . Biotechnology Advances , 2018 , 36 ( 5 ): 1424 - 1433 .
QIU Y M , ZHANG J Y , LI L , et al . Engineering Bacillus licheniformis for the production of meso -2,3-butanediol [J ] . Biotechnology for Biofuels , 2016 , 9 ( 1 ): 117 .
QI G F , KANG Y F , LI L , et al . Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis [J ] . Biotechnology for Biofuels , 2014 , 7 ( 1 ): 16 .
VEITH B , HERZBERG C , STECKEL S , et al . The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential [J ] . Journal of Molecular Microbiology and Biotechnology , 2004 , 7 ( 4 ): 204 - 211 .
ZHOU C X , LIU H , YUAN F Y , et al . Development and application of a CRISPR/Cas9 system for Bacillus licheniformis genome editing [J ] . International Journal of Biological Macromolecules , 2019 , 122 : 329 - 337 .
ZHAN Y Y , XU Y , ZHENG P L , et al . Establishment and application of multiplexed CRISPR interference system in Bacillus licheniformis [J ] . Applied Microbiology and Biotechnology , 2020 , 104 ( 1 ): 391 - 403 .
WANG H , YANG L , PING Y H , et al . Engineering of a Bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions [J ] . PLoS One , 2016 , 11 ( 1 ): e0146373 .
SHA Y Y , HUANG Y Y , ZHU Y F , et al . Efficient biosynthesis of low-molecular-weight poly- γ -glutamic acid based on stereochemistry regulation in Bacillus amyloliquefaciens [J ] . ACS Synthetic Biology , 2020 , 9 ( 6 ): 1395 - 1405 .
ZHANG F , HUO K Y , SONG X Y , et al . Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production [J ] . Microbial Cell Factories , 2020 , 19 ( 1 ): 223 .
YANG N , WU Q , XU Y . Fe nanoparticles enhanced surfactin production in Bacillus amyloliquefaciens [J ] . ACS Omega , 2020 , 5 ( 12 ): 6321 - 6329 .
GU Y Y , ZHENG J Y , FENG J , et al . Improvement of levan production in Bacillus amyloliquefaciens through metabolic optimization of regulatory elements [J ] . Applied Microbiology and Biotechnology , 2017 , 101 ( 10 ): 4163 - 4174 .
JIANG C , RUAN L Y , WEI X T , et al . Enhancement of S -adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens [J ] . Biotechnology Letters , 2020 , 42 ( 11 ): 2293 - 2298 .
SAMUEL M S , JOSE S , SELVARAJAN E , et al . Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens ; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p -nitrophenol [J ] . Journal of Photochemistry and Photobiology B: Biology , 2020 , 202 : 111642 .
INUI M , KAWAGUCHI H , MURAKAMI S , et al . Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions [J ] . Journal of Molecular Microbiology and Biotechnology , 2004 , 8 ( 4 ): 243 - 254 .
SASAKI M , JOJIMA T , KAWAGUCHI H , et al . Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars [J ] . Applied Microbiology and Biotechnology , 2009 , 85 ( 1 ): 105 - 115 .
KITADE Y , HASHIMOTO R , SUDA M , et al . Production of 4-hydroxybenzoic acid by an aerobic growt h-arrested bioprocess using metabolically engineered Corynebacterium glutamicum [J ] . Applied and Environmental Microbiology , 2018 , 84 ( 6 ).
KUBOTA T , WATANABE A , SUDA M , et al . Production of para-aminobenzoate by genetically engineered Corynebacterium glutamicum and non-biological formation of an N -glucosyl byproduct [J ] . Metabolic Engineering , 2016 , 38 : 322 - 330 .
CHOI J W , JEON E J , JEONG K J . Recent advances in engineering Corynebacterium glutamicum for utilization of hemicellulosic biomass [J ] . Current Opinion in Biotechnology , 2019 , 57 : 17 - 24 .
JORGE J M P , PÉREZ-GARCÍA F , WENDISCH V F . A new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources [J ] . Bioresource Technology , 2017 , 245 ( B ): 1701 - 1709 .
BARITUGO K A , KIM H T , DAVID Y , et al . Metabolic engineering of Corynebacterium glutamicum for fermentative production of chemicals in biorefinery [J ] . Applied Microbiology and Biotechnology , 2018 , 102 ( 9 ): 3915 - 3937 .
SCHÄFER A , TAUCH A , JÄGER W , et al . Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum [J ] . Gene , 1994 , 145 ( 1 ): 69 - 73 .
SUZUKI N , NONAKA H , TSUGE Y , et al . New multiple-deletion method for the Corynebacterium glutamicum genome, using a mutant lox sequence [J ] . Applied and Environmental Microbiology , 2005 , 71 ( 12 ): 8472 - 8480 .
WANG Y , LIU Y , LIU J , et al . MACBETH: Multiplex automated Corynebacterium glutamicum base editing method [J ] . Metabolic Engineering , 2018 , 47 : 200 - 210 .
HEIDER S A E , WENDISCH V F . Engineering microbial cell factories: metabolic engineering of Corynebacterium glutamicum with a focus on non-natural products [J ] . Biotechnology Journal , 2015 , 10 ( 8 ): 1170 - 1184 .
BELL S P , LABIB K . Chromosome duplication in Saccharomyces cerevisiae [J ] . Genetics , 2016 , 203 ( 3 ): 1027 - 1067 .
ZHANG W P , DU G C , ZHOU J W , et al . Regulation of sensing, transportation, and catabolism of nitrogen sources in Saccharomyces cerevisiae [J ] . Microbiology and Molecular Biology Reviews , 2018 , 82 ( 1 ): e00040-17 .
LAUN P , PICHOVA A , MADEO F , et al . Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis [J ] . Molecular Microbiology , 2001 , 39 ( 5 ): 1166 - 1173 .
HO Y , GRUHLER A , HEILBUT A , et al . Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry [J ] . Nature , 2002 , 415 ( 6868 ): 180 - 183 .
UETZ P , GIOT L , CAGNEY G , et al . A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae [J ] . Nature , 2000 , 403 ( 6770 ): 623 - 627 .
KROGAN N J , CAGNEY G , YU H Y , et al . Global landscape of protein complexes in the yeast Saccharomyces cerevisiae [J ] . Nature , 2006 , 440 ( 7084 ): 637 - 643 .
NEIMAN A M . Ascospore formation in the yeast Saccharomyces cerevisiae [J ] . Microbiology and Molecular Biology Reviews , 2005 , 69 ( 4 ): 565 - 584 .
LEVIN D E . Cell wall integrity signaling in Saccharomyces cerevisiae [J ] . Microbiology and Molecular Biology Reviews , 2005 , 69 ( 2 ): 262 - 291 .
YAMANISHI M , ITO Y , KINTAKA R , et al . A genome-wide activity assessment of terminator regions in Saccharomyces cerevisiae provides a ''terminatome'' toolbox [J ] . ACS Synthetic Biology , 2013 , 2 ( 6 ): 337 - 347 .
TEIXEIRA M C , MONTEIRO P T , PALMA M , et al . YEASTRACT: an upgraded database for the analysis of transcription regulatory networks in Saccharomyces cerevisiae [J ] . Nucleic Acids Research , 2018 , 46 ( D1 ): D348 - D353 .
BALAKRISHNAN R , PARK J , KARRA K , et al . YeastMine—an integrated data warehouse for Saccharomyces cerevisiae data as a multipurpose tool-kit [J ] . Database , 2012 , 2012 : bar062 .
SHAO Z Y , ZHAO H , ZHAO H M . DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways [J ] . Nucleic Acids Research , 2009 , 37 ( 2 ): e16 - e16 .
KUIJPERS N G , SOLIS-ESCALANTE D , BOSMAN L , et al . A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences [J ] . Microbial Cell Factories , 2013 , 12 : 47 .
HONG K K , NIELSEN J . Metabolic engineering of Saccharomyces cerevisiae : a key cell factory platform for future biorefineries [J ] . Cellular and Molecular Life Sciences , 2012 , 69 ( 16 ): 2671 - 2690 .
KAYIKCI Ö , NIELSEN J . Glucose repression in Saccharomyces cerevisiae [J ] . FEMS Yeast Research , 2015 , 15 ( 6 ): fov068 .
HOU J , QIU C X , SHEN Y , et al . Engineering of Saccharomyces cerevisiae for the efficient co-utilization of glucose and xylose [J ] . FEMS Yeast Research , 2017 , 17 ( 4 ): fox034 .
KOZAK B U , VAN ROSSUM H M , LUTTIK M A H , et al . Engineering acetyl coenzyme A supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of Saccharomyces cerevisiae [J ] . mBio , 2014 , 5 ( 5 ): e01696 - e01614 .
JIANG G Z , YAO M D , WANG Y , et al . Manipulation of GES and E RG20 for geraniol overproduction in Saccharomyces cerevisiae [J ] . Metabolic Engineering , 2017 , 41 : 57 - 66 .
CHENG S , LIU X , JIANG G Z , et al . Orthogonal engineering of biosynthetic pathway for efficient production of limonene in Saccharomyces cerevisiae [J ] . ACS Synthetic Biology , 2019 , 8 ( 5 ): 968 - 975 .
DAI Z B , LIU Y , ZHANG X N , et al . Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides [J ] . Metabolic Engineering , 2013 , 20 : 146 - 156 .
PADDON C J , WESTFALL P J , PITERA D J , et al . High-level semi-synthetic production of the potent antimalarial artemisinin [J ] . Nature , 2013 , 496 ( 7446 ): 528 - 532 .
LUO X , REITER M A , D'ESPAUX L , et al . Complete biosynthesis of cannabinoids and their unnatural analogues in yeast [J ] . Nature , 2019 , 567 ( 7746 ): 123 - 126 .
GOTTARDI M , REIFENRATH M , BOLES E , et al . Pathway engineering for the production of heterologous aromatic chemicals and their derivatives in Saccharomyces cerevisiae : bioconversion from glucose [J ] . FEMS Yeast Research , 2017 , 17 ( 4 ): fox035 .
KRIVORUCHKO A , NIELSEN J . Production of natural products through metabolic engineering of Saccharomyces cerevisiae [J ] . Current Opinion in Biotechnology , 2015 , 35 : 7 - 15 .
LUTTIK M AH , VURALHAN Z , SUIR E , et al . Alleviation of feedback inhibition in Saccharomyces cerevisiae aromatic amino acid biosynthesis: quantification of metabolic impact [J ] . Metabolic Engineering , 2008 , 10 ( 3/4 ): 141 - 153 .
DEVER T E , KINZY T G , PAVITT G D . Mechanism and regulation of protein synthesis in Saccharomyces cerevisiae [J ] . Genetics , 2016 , 203 ( 1 ): 65 - 107 .
RASALA B A , MAYFIELD S P . Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses [J ] . Photosynthesis Research , 2015 , 123 ( 3 ): 227 - 239 .
DE SCHUTTER K , LIN Y C , TIELS P , et al . Genome sequence of the recombinant protein production host Pichia pastoris [J ] . Nature Biotechnology , 2009 , 27 ( 6 ): 561 - 566 .
SCHWARZHANS J P , LUTTERMANN T , GEIER M , et al . Towards systems metabolic engineering in Pichia pastoris [J ] . Biotechnology Advances , 2017 , 35 ( 6 ): 681 - 710 .
韩明哲 , 陈为刚 , 宋理富 , 等 . DNA信息存储: 生命系统与信息系统的桥梁 [J ] . 合成生物学 , 2021 , 2 ( 3 ): 309 - 322 .
HAN M Z , CHEN W G , SONG L F , et al . DNA information storage: bridging biological and digital world [J ] . Synthetic Biology Journal , 2021 , 2 ( 3 ): 309 - 322 .
张媛媛 , 曾艳 , 王钦宏 . 合成生物制造进展 [J ] . 合成生物学 , 2021 , 2 ( 2 ): 145 - 160 .
ZHANG Y Y , ZENG Y , WANG Q H . Advances in synthetic biomanufacturing [J ] . Synthetic Biology Journal , 2021 , 2 ( 2 ): 145 - 160 .
0
Views
4
下载量
5
CSCD
- L-PDFDownload
- WORDDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Postal code：100000
- Tel：010-64519339
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.