Research progress of cyclic lipopeptide biosynthesis

HOU Zhengjie; SUN Huizhong; BAI Song; CHEN Xinyue; CAO Chunyang; CHENG Jingsheng

doi:10.12211/2096-8280.2021-008

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Research progress of cyclic lipopeptide biosynthesis

Invited Review | 更新时间：2026-03-05

- Research progress of cyclic lipopeptide biosynthesis
- Synthetic Biology Journal Vol. 2, Issue 4, Pages: 577-597(2021)
- 作者机构：
  
  教育部合成生物学前沿科学中心，系统生物学教育部重点实验室，天津大学化工学院，天津 300350
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2021-008
  CLC： Q815
- Received：20 January 2021，
  
  Revised：2021-04-03，
  
  Published：31 August 2021
- 稿件说明：
移动端阅览
侯正杰，孙慧中，白松，陈新月，曹春阳，程景胜. 环脂肽生物合成的研究进展［J］. 合成生物学， 2021， 2（4）： 577-597

HOU Zhengjie， SUN Huizhong， BAI Song， CHEN Xinyue， CAO Chunyang， CHENG Jingsheng. Research progress of cyclic lipopeptide biosynthesis［J］. Synthetic Biology Journal， 2021， 2（4）： 577-597
侯正杰，孙慧中，白松，陈新月，曹春阳，程景胜. 环脂肽生物合成的研究进展［J］. 合成生物学， 2021， 2（4）： 577-597 DOI： 10.12211/2096-8280.2021-008.

HOU Zhengjie， SUN Huizhong， BAI Song， CHEN Xinyue， CAO Chunyang， CHENG Jingsheng. Research progress of cyclic lipopeptide biosynthesis［J］. Synthetic Biology Journal， 2021， 2（4）： 577-597 DOI： 10.12211/2096-8280.2021-008.

摘要

环脂肽化合物是一类结构新颖的环状肽类，其两亲性的物化特性决定了其独特的生物活性，可作为抗生素、生物表面活性剂等。在生物防治、药物开发、环境修复和疾病治疗等方面广泛应用，具有迫切的市场需求和广阔的发展前景。环脂肽类天然产物主要由非核糖体肽合成途径合成，由于环脂肽合成复杂的代谢网络和前体需求、专一且严格的合成途径、多种同系物的共存，制约着环脂肽合成的微生物开发和产品价值提升。本文主要介绍了来源于细菌界的环脂肽类物质的结构特性，非核糖体肽合成途径及非核糖体肽合成酶（non-ribosomal peptide synthetase，NRPS）的结构域特点，天然产物底盘菌株开发现状，通过基因工程、代谢工程方法进行同系物调控和生物合成策略，混菌对脂肽生物合成的影响，以及合成生物学在脂肽合成中的应用。随着合成生物技术的迅速发展和运用，环脂肽类天然产物的微生物合成也有望实现“质”和“量”的提升，以及促进新型脂肽的开发。

Abstract

As antibiotics and bio-surfactants

cyclic lipopeptides have unique molecular structure and biological activity and are widely applied in the fields of biological control

drug development

environmental remediation and disease treatment. It has vigorous market demand and promising future. Cyclic lipopeptides are a class of antibiotics synthesized from non-ribosomal peptide pathways by microorganisms. However

the complex metabolic network and precursor requirements

specific and strict synthetic pathway

and the coexistence of multiple homologues are restricting our capability of developing the lipopeptide syntheses potential of microorganism and promoting the product value of lipopeptide of bacteria. In this paper

we summarize the types of chassis cells for producing cyclic lipopeptides. We also introduce the structural characteristics of cyclic lipopeptides based on bacterial origin

synthesis pathway of non-ribosomal peptide

and structural domain characteristics of non-ribosomal peptide synthetase. In addition

the strategies for homologues regulation and biosynthetic yield improvement through genetic and metabolic engineering methods were reviewed

as well as the development status of natural product chassis strains. The synthesis of lipopeptide products can be effectively improved by optimizing the precursor metabolism

enhancing the expression of lipopeptide synthesis gene cluster

blocking the competitive pathway of lipopeptide synthesis

and the modification of various regulatory factors. The non-ribosomal peptide synthetase structural domain can be modified to obtain higher value lipopeptide and new lipopeptide drugs. We also review the effects of mixed-culture on lipopeptide syntheses

the development status of chassis strains for producing natural product

and the application of synthetic biology for improving lipopeptide biosynthesis. With the rapid development and application of synthetic biotechnology

the quality and quantity of natural lipopeptide from microorganisms will be improved rapidly. It also boosts the development of novel cyclic lipopeptides. And a better understanding of the synthesis

modification and mechanism of action of antimicrobial peptides will restart its commercial development.

关键词

Keywords

references

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