Strategies of cell factory construction for the production of aromatic amino acids and their derivatives

SUN Wei; DING Dongqin; BAI Danyang; ZHU Yaru; XIE Xiaotong; ZHANG Dawei

doi:10.12211/2096-8280.2021-002

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Strategies of cell factory construction for the production of aromatic amino acids and their derivatives

Invited Review | 更新时间：2025-03-20

- Strategies of cell factory construction for the production of aromatic amino acids and their derivatives
- Synthetic Biology Journal Vol. 2, Issue 6, Pages: 982-999(2021)
- 作者机构：
  
  1.天津科技大学生物工程学院，天津 300457
  2.中国科学院天津工业生物技术研究所，天津 300308
  3.大连工业大学生物工程学院，辽宁大连 116000
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2021-002
  CLC： Q81
- Received：05 January 2021，
  
  Revised：2021-04-16，
  
  Published：31 December 2021
- 稿件说明：
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孙薇，丁冬芹，柏丹阳，朱亚如，解晓彤，张大伟. 芳香族氨基酸及其衍生物的细胞工厂构建策略［J］. 合成生物学， 2021， 2（6）： 982-999

SUN Wei， DING Dongqin， BAI Danyang， ZHU Yaru， XIE Xiaotong， ZHANG Dawei. Strategies of cell factory construction for the production of aromatic amino acids and their derivatives［J］. Synthetic Biology Journal， 2021， 2（6）： 982-999
孙薇，丁冬芹，柏丹阳，朱亚如，解晓彤，张大伟. 芳香族氨基酸及其衍生物的细胞工厂构建策略［J］. 合成生物学， 2021， 2（6）： 982-999 DOI： 10.12211/2096-8280.2021-002.

SUN Wei， DING Dongqin， BAI Danyang， ZHU Yaru， XIE Xiaotong， ZHANG Dawei. Strategies of cell factory construction for the production of aromatic amino acids and their derivatives［J］. Synthetic Biology Journal， 2021， 2（6）： 982-999 DOI： 10.12211/2096-8280.2021-002.

摘要

芳香族氨基酸及其衍生物由于其特定的生理活性，已广泛应用于医药、食品、饲料和化工等行业。利用重组微生物发酵生产芳香族氨基酸及其衍生物是满足全球日益增长需求的有效途径。通过将代谢工程策略与合成生物学、系统生物学和生物工程的发展相结合，在菌株的改造及优化方面取得了显著的进展。然而，合成芳香族氨基酸及其衍生物的代谢途径长且调控机制复杂，通过简单的代谢途径改造难以大幅提高产量，因此，近年来出现了很多相关的改造方法，为克服代谢途径中的限速问题提供了很好的借鉴意义。本文回顾和比较了最近在芳香族氨基酸及其衍生物合成方面应用的成熟技术和策略，包括常用的代谢途径改造策略（如增加前体供给、解除关键酶和阻遏蛋白的反馈抑制和阻遏抑制、改造转运系统、全局调节系统）以及菌株生长与生产产品耦联和菌株构建方法（如基于生物传感器的高通量筛选以及对培养基和培养条件的优化等），未来相关前沿技术如计算机辅助途径酶改造技术和筛选高产菌株的定向进化技术将助力芳香族氨基酸及其衍生物高产菌株的构建。

Abstract

Aromatic amino acids (including L-tryptophan

L-phenylalanine and L-tyrosine) and their derivatives have been widely used in medicine

food

feed and chemical industry due to their specific physiological properties. The production of aromatic amino acids and their derivatives by recombinant microbial fermentation is an effective way to meet the increasing global demand. By combining metabolic engineering strategy with the developments of synthetic biology

systems biology and bioengineering

remarkable progress has been made in the strains modifications and improvements. However

the metabolic pathways for the synthesis of aromatic amino acids and their derivatives are long and their regulatory mechanisms are complicated

so it is very difficult to significantly improve the yield through simple metabolic pathway-modification. Therefore

many relevant modification methods have emerged in recent years

providing a good reference for overcoming the rate limit problem in the metabolic pathways. In this paper

we review and compare the recent mature technologies and strategies applied in the synthesis of aromatic amino acids and their derivatives

including the commonly used metabolic pathway modification strategies

such as increasing the supply of precursors

removing the feedback inhibition for key enzymes

eliminating the repression of repressor proteins

regulating the transport system and global metabolic network

the coupling of strain's growth and product's production and introducing exogenous related enzymes and so on. Various methods of strain construction are also included

such as the high-throughput screening based on biosensors and optimization of culture medium and culture conditions and so on. Finally

we also discuss the prospect of relevant cutting-edge technologies such as computer design of protein

computer de novo design of enzyme

computer design of metabolic pathway

alphafold algorithm for accurate prediction of protein structure and bifunctional enzyme

and the directed evolution technology for screening high yield strains such as the batch and continuation of the directed evolution.

关键词

Keywords

references

鄢芳清 , 韩亚昆 , 李娟 , 等 . 大肠杆菌芳香族氨基酸代谢工程研究进展 [J ] . 生物加工过程 , 2017 , 15 ( 5 ): 32 - 39,85 .

YAN F Q , HAN K , LI J , et al . Metabolic engineering of aromatic amino acids in Escherichia coli [J ] . Chinese Journal of Bioprocess Engineering , 2017 , 15 ( 5 ): 32 - 39,85 .

于金龙 . 大肠杆菌芳香族氨基酸生物合成途径的代谢调控研究 [D ] . 北京 : 中国人民解放军军事医学科学院 , 2008 .

YU J L . Metabolic regulation of aromatic amino acid biosynthesis pathway in Escherichia coli [D ] . Beijing : Chinese Academy of Military Sciences , 2008 .

吴凤礼 , 彭彦峰 , 徐毅诚 , 等 . 代谢工程改造微生物生产芳香族化合物的研究进展 [J ] . 生物加工过程 , 2017 , 15 ( 5 ): 9 - 23 .

WU F L , PENG Y F , XU Y C , et al . Advances in microbial metabolic engineering for producing aromatic chemicals [J ] . Chinese Journal of Bioprocess Engineering , 2017 , 15 ( 5 ): 9 - 23 .

ZHANG R H , LI C Y , WANG J , et al . Microbial production of small medicinal molecules and biologics: From nature to synthetic pathways [J ] . Biotechnology Advances , 2018 , 36 ( 8 ): 2219 - 2231 .

李飞飞 , 赵广荣 . 大肠杆菌代谢工程生产芳香族化合物研究进展 [J ] . 食品与发酵工业 , 2014 , 40 ( 6 ): 128 - 134 .

LI F F , ZHAO G R . Advances in metabolic engineering of Escherichia coli for producing aromatic compounds [J ] . Food and Fermentation Industries , 2014 , 40 ( 6 ): 128 - 134 .

PITTARD J , YANG J . Biosynthesis of the aromatic amino acids [J ] . EcoSal Plus , 2008 , 3 ( 1 ): 285 - 356 .

JOO J C , OH Y H , YU J H , et al . Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process [J ] . Bioresource Technology , 2017 , 245 ( Pt B ): 1692 - 1700 .

WILLKE T . Methionine production—a critical review [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 24 ): 9893 - 9914 .

GRÖGER H . Biocatalytic concepts for synthesizing amine bulk chemicals: recent approaches towards linear and cyclic aliphatic primary amines and ω -substituted derivatives thereof [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 1 ): 83 - 95 .

WENDISCH V F . Metabolic engineering advances and prospects for amino acid production [J ] . Metabolic Engineering , 2020 , 58 ( 1 ): 17 - 34 .

WENDISCH V F . Microbial production of amino acids and derived chemicals: synthetic biology approaches to strain development [J ] . Current Opinion in Biotechnology , 2014 , 30 ( 3 ): 51 - 58 .

申晓林 , 袁其朋 . 生物合成芳香族氨基酸及其衍生物的研究进展 [J ] . 生物技术通报 , 2017 , 33 ( 1 ): 24 - 34 .

SHEN X L , YUAN Q P . Research advance on biosynthesis of aromatic amino acids and their derivatives [J ] . Biotechnology Bulletin , 2017 , 33 ( 1 ): 24 - 34 .

赵志军 . L-色氨酸生产菌株的构建及代谢调控研究 [D ] . 无锡 : 江南大学 , 2011 .

ZHAO Z J . Construction and metabolic regulation of L-tryptophan producing strain [D ] . Wuxi : Jiangnan University , 2011 .

BONGAERTS J , KRÄMER M , MÜLLER U , et al . Metabolic engineering for microbial production of aromatic amino acids and derived compounds [J ] . Metabolic Engineering , 2001 , 3 ( 4 ): 289 - 300 .

王健 , 陈宁 . 基于途径分析及代谢流量分析的L-色氨酸发酵条件优化 [J ] . 云南大学学报(自然科学版) , 2004 , 26 ( S2 ): 68 - 73 .

WANG J , CHEN N . Optimization of L-tryptophan fermentation conditions based on pathway analysis and metabolic flux analysis [J ] . Journal of Yunnan University (Natural Science Edition) , 2004 , 26 ( S2 ): 68 - 73 .

FLORES S , GOSSET G , FLORES N , et al . Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13 C labeling and NMR spectroscopy [J ] . Metabolic Engineering , 2002 , 4 ( 2 ): 124 - 137 .

汪多仁 . L-苯丙氨酸生产与应用 [J ] . 发酵科技通讯 , 2011 , 40 ( 1 ): 29 - 36 .

WANG D R . Production and application of L-phenylalanine [J ] . Fermentation Technology Communication , 2011 , 40 ( 1 ): 29 - 36 .

LIU Q L , YU T , LI X W , et al . Rewiring carbon metabolism in yeast for high level production of aromatic chemicals [J ] . Nature Communications , 2019 , 10 ( 1 ): 4976 .

PONCE E , MARTÍNEZ A , BOLÍVAR F , et al . Stimulation of glucose catabolism through the pentose pathway by the absence of the two pyruvate kinase isoenzymes in Escherichia coli [J ] . Biotechnology and Bioengineering , 1998 , 58 ( 2/3 ): 292 - 295 .

SPRAGGON G , KIM C , NGUYEN-HUU X , et al . The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, L-tryptophan [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2001 , 98 ( 11 ): 6021 - 6026 .

BRAUS G H . Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae : a model system for the regulation of a eukaryotic biosynthetic pathway [J ] . Microbiological Reviews , 1991 , 55 ( 3 ): 349 - 370 .

WALLACE B J , PITTARD J . Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli [J ] . Journal of Bacteriology , 1969 , 97 ( 3 ): 1234 - 1241 .

SINGLETON C K , ROEDER W D , BOGOSIAN G , et al . DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor [J ] . Nucleic Acids Research , 1980 , 8 ( 7 ): 51 - 60 .

YANOFSKY C , HORN V , GOLLNICK P . Physiological studies of tryptophan transport and tryptophanase operon induction in Escherichia coli [J ] . Journal of Bacteriology , 1991 , 173 ( 19 ): 6009 - 6017 .

IKEDA M , KATSUMATA R . Tryptophan production by transport mutants of Corynebacterium glutamicum [J ] . Bioscience, Biotechnology, and Biochemistry , 1995 , 59 ( 8 ): 1600 - 1602 .

DOROSHENKO V , AIRICH L , VITUSHKINA M , et al . YddG from Escherichia coli promotes export of aromatic amino acids [J ] . FEMS Microbiology Letters , 2007 , 275 ( 2 ): 312 - 318 .

AIBA S , TSUNEKAWA H , IMANAKA T . New approach to tryptophan production by Escherichia coli : genetic manipulation of composite plasmids in vitro [J ] . Applied and Environmental Microbiology , 1982 , 43 ( 2 ): 289 - 297 .

SHI S , CHEN T , ZHANG Z , et al . Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production [J ] . Metabolic Engineering , 2009 , 11 ( 4/5 ): 243 - 252 .

LÜTKE-EVERSLOH T , STEPHANOPOULOS G . Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli : identification of enzymatic bottlenecks by systematic gene overexpression [J ] . Metabolic Engineering , 2008 , 10 ( 2 ): 69 - 77 .

OH J S , PARK H H , PARK T H . Temperature management strategy for efficient gene expression in a thermally inducible Escherichia coli /bacteriophage system [J ] . Biotechnology and Bioprocess Engineering , 2008 , 13 ( 4 ): 470 - 475 .

SUÁSTEGUI M , GUO W H , FENG X Y , et al . Investigating strain dependency in the production of aromatic compounds in Saccharomyces cerevisiae [J ] . Biotechnology and Bioengineering , 2016 , 113 ( 12 ): 2676 - 2685 .

PATNAIK R , SPITZER R G , LIAO J C . Pathway engineering for production of aromatics in Escherichia coli : confirmation of stoichiometric analysis by independent modulation of AroG, TktA, and Pps activities [J ] . Biotechnology and Bioengineering , 1995 , 46 ( 4 ): 361 - 370 .

CARMONA S B , MORENO F , BOLÍVAR F , et al . Inactivation of the PTS as a strategy to engineer the production of aromatic metabolites in Escherichia coli [J ] . Journal of Molecular Microbiology & Biotechnology , 2015 , 25 ( 2/3 ): 195 - 208 .

CHEN Y Y , LIU Y F , DING D Q , et al . Rational design and analysis of an Escherichia coli strain for high-efficiency tryptophan production [J ] . Journal of Industrial Microbiology and Biotechnology , 2018 , 45 ( 5 ): 357 - 367 .

CARMONA S B , FLORES N , MARTÍNEZ-ROMERO E , et al . Evolution of an Escherichia coli PTS-strain: a study of reproducibility and dynamics of an adaptive evolutive process [J ] . Applied Microbiology and Biotechnology , 2020 , 104 ( 21 ): 9309 - 9325 .

CHEN L , ZENG A P . Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration [J ] . Applied Microbiology and Biotechnology , 2017 , 101 ( 2 ): 559 - 568 .

吴涛 , 赵津津 , 毛贤军 . 大肠杆菌磷酸烯醇式丙酮酸-糖磷酸转移酶系统改造对产L-色氨酸的影响 [J ] . 生物工程学报 , 2017 , 33 ( 11 ): 1877 - 1882 .

WU T , ZHAO J J , MAO X J . Effect of PTS modifications on L-tryptophan production in Escherichia coli [J ] . Chinese Journal of Biotechnology , 2017 , 33 ( 11 ): 1877 - 1882 .

GOSSET G , YONG-XIAO J , BERRY A . A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli [J ] . Journal of Industrial Microbiology , 1996 , 17 ( 1 ): 47 - 52 .

BERRY A . Improving production of aromatic compounds in Escherichia coli by metabolic engineering [J ] . Trends in Biotechnology , 1996 , 14 ( 7 ): 250 - 256 .

DU L H , ZHANG Z , XU Q Y , et al . Central metabolic pathway modification to improve L-tryptophan production in Escherichia coli [J ] . Bioengineered , 2019 , 10 ( 1 ): 59 - 70 .

吴永庆 , 江培翊 , 范长胜 , 等 . 大肠杆菌 ppsA , pckA 基因的克隆与串联表达 [J ] . 复旦学报(自然科学版) , 2002 , 41 ( 1 ): 31 - 35 .

WU Y Q , JIANG P X , FAN C S , et al . Cloning and co-expression of ppsA and pckA genes in Escherichia coli [J ] . Journal of Fudan University (Natural Sciences) , 2002 , 41 ( 1 ): 31 - 35 .

TYAGI N , SAINI D , GULERIA R , et al . Designing an Escherichia coli strain for phenylalanine overproduction by metabolic engineering [J ] . Molecular Biotechnology , 2017 , 59 ( 4/5 ): 168 - 178 .

MASCARENHAS D , ASHWORTH D J , CHEN C S . Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli [J ] . Applied and Environmental Microbiology , 1991 , 57 ( 10 ): 2995 - 2999 .

GUO W , HUANG Q L , FENG Y H , et al . Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae [J ] . Biotechnology and Bioengineering , 2020 , 117 ( 8 ): 2410 - 2419 .

TRENCHARD I J , SMOLKE C D . Engineering strategies for the fermentative production of plant alkaloids in yeast [J ] . Metabolic Engineering , 2015 , 30 : 96 - 104 .

JOSSEK R , BONGAERTS J , SPRENGER G A . Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli [J ] . FEMS Microbiology Letters , 2001 , 202 ( 1 ): 145 - 148 .

LIU L N , DUAN X G , WU J . Modulating the direction of carbon flow in Escherichia coli to improve L-tryptophan production by inactivating the global regulator FruR [J ] . Journal of Biotechnology , 2016 , 231 ( 4 ): 138 - 141 .

李剑欣 , 郭长江 , 刘云 , 等 . 大肠杆菌邻氨基苯甲酸合成酶编码基因 trpED 的克隆与表达 [J ] . 生物技术通讯 , 2007 , 18 ( 2 ): 183 - 185 .

LI J X , GUO C J , LIU Y , et al . The clone and expression of the anthranilate synthetase coding gene in Escherichia coli [J ] . Letters in Biotechnology , 2007 , 18 ( 2 ): 183 - 185 .

IKEDA M . Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering [J ] . Applied Microbiology and Biotechnology , 2006 , 69 ( 6 ): 615 - 626 .

LÜTKE-EVERSLOH T , STEPHANOPOULOS G . L-tyrosine production by deregulated strains of Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2007 , 75 ( 1 ): 103 - 110 .

LIU Y J , LI P P , ZHAO K X , et al . Corynebacterium glutamicum contains 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that display novel biochemical features [J ] . Applied and Environmental Microbiology , 2008 , 74 ( 17 ): 5497 - 5503 .

RODRIGUEZ A , CHEN Y , KHOOMRUNG S , et al . Comparison of the metabolic response to over-production of p -coumaric aci d in two yeast strains [J ] . Metabolic Engineering , 2017 , 44 : 265 - 272 .

SCHENCK C A , MAEDA H A . Tyrosine biosynthesis, metabolism, and catabolism in plants [J ] . Phytochemistry , 2018 , 149 : 82 - 102 .

LÜTKE-EVERSLOH T , STEPHANOPOULOS G . Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli : generation and characterization of tyrosine-insensitive mutants [J ] . Applied and Environmental Microbiology , 2005 , 71 ( 11 ): 7224 - 7228 .

DODGE T C , GERSTNER J M . Optimization of the glucose feed rate profile for the production of tryptophan from recombinant E coli [J ] . Journal of Chemical Technology and Biotechnology , 2002 , 77 ( 11 ): 45 - 123 .

LIU S P , LIU R X , XIAO M R , et al . A systems level engineered E. coli capable of efficiently producing L-phenylalanine [J ] . Process Biochemistry , 2014 , 49 ( 5 ): 751 - 757

FERNSTROM J D . A perspective on the safety of supplemental tryptophan based on its metabolic fates [J ] . The Journal of Nutrition , 2016 , 146 ( 12 ): 2601S - 2608S .

古鹏飞 . 利用重组大肠杆菌生产L-色氨酸的研究 [D ] . 济南 : 山东大学 , 2013 .

GU P F . Production of L-tryptophan by recombinant Escherichia coli [D ] . Jinan : Shandong University , 2013 .

MUÑOZ A J , HERNÁNDEZ-CHÁVEZ G , DE ANDA R , et al . Metabolic engineering of Escherichia coli for improving 1-3,4-dihydroxyphenylalanine (L-Dopa) synthesis from glucose [J ] . Journal of Industrial Microbiology & Biotechnology , 2011 , 38 ( 11 ): 1845 - 1852 .

KIM B , BINKLEY R , KIM H U , et al . Metabolic engineering of Escherichia coli for the enhanced production of L-tyrosine [J ] . Biotechnology and Bioengineering , 2018 , 115 ( 10 ): 2554 - 2564 .

LIU Y F , XU Y R , DING D Q , et al . Genetic engineering of Escherichia coli to improve L-phenylalanine production [J ] . BMC Biotechnology , 2018 , 18 ( 1 ): 5 .

SUN Z T , NING Y Y , LIU L X , et al . Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S - or R -mandelic acid [J ] . Microbial Cell Factories , 2011 , 10 ( 1 ): 71 .

FUJITA T , NGUYEN H D , ITO T , et al . Microbial monomers custom-synthesized to build true bio-derived aromatic polymers [J ] . Applied Microbiology and Biotechnology , 2013 , 97 ( 20 ): 8887 - 8894 .

BANG H B , LEE Y H , KIM S C , et al . Metabolic engineering of Escherichia coli for the production of cinnamaldehyde [J ] . Microbial Cell Factories , 2016 , 15 ( 1 ): 16 .

GOTTARDI M , KNUDSEN J D , PRADO L , et al . De novo biosynthesis of trans-cinnamic acid derivatives in Saccharomyces cerevisiae [J ] . Applied Microbiology and Biotechnology , 2017 , 101 ( 12 ): 4883 - 4893 .

MCKENNA R , NIELSEN D R . Styrene biosynthesis from glucose by engineered E. coli [J ] . Metabolic Engineering , 2011 , 13 ( 5 ): 544 - 554 .

ROMASI E F , LEE J . Development of indole-3-acetic acid-producing Escherichia coli by functional expression of IpdC, AspC, and Iad1 [J ] . Journal of microbiology and biotechnology , 2013 , 23 ( 12 ): 26 - 36 .

SUN H , ZHAO D , XIONG B , et al . Engineering Corynebacterium glutamicum for violacein hyper production [J ] . Microbial Cell Factories , 2016 , 15 ( 1 ): 148 .

RODRIGUES A L , TRACHTMANN N , BECKER J , et al . Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein [J ] . Metabolic Engineering , 2013 , 20 : 29 - 41 .

PARK S , KANG K , LEE S W , et al . Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2011 , 89 ( 5 ): 1387 - 1394 .

韩晓红 , 王伟 , 肖兴国 . 靛蓝及其同类色素的微生物生产与转化 [J ] . 生物工程学报 , 2008 , 24 ( 6 ): 921 - 926 .

HAN X H , WANG W , XIAO X G . Microbial biosynthesis and biotransformation of indigo and indigo-like pigments [J ] . Chinese Journal of Biotechnology , 2008 , 24 ( 6 ): 921 - 926 .

YAO Y F , WANG C S , QIAO J , et al . Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway [J ] . Metabolic Engineering , 2013 , 19 : 79 - 87 .

QI W W , VANNELLI T , BREINIG S , et al . Functional expression of prokaryotic and eukaryotic genes in Escherichia coli for conversion of glucose to p -hydroxystyrene [J ] . Metabolic Engineering , 2007 , 9 ( 3 ): 268 - 276 .

NIU H , LI R R , LIANG Q F , et al . Metabolic engineering for improving L-tryptophan production in Escherichia coli [J ] . Journal of Industrial Microbiology & Biotechnology , 2019 , 46 ( 1 ): 55 - 65 .

PÉREZ-GARCÍA F , WENDISCH V F . Transport and metabolic engineering of the cell factory Corynebacterium glutamicum [J ] . FEMS Microbiology Letters , 2018 , 365 ( 16 ): fny166 .

GU P F , YANG F , LI F F , et al . Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production [J ] . Applied Microbiology and Biotechnology , 2013 , 97 ( 15 ): 6677 - 6683 .

TATARKO M , ROMEO T . Disruption of a global regulatory gene to enhance central carbon flux into phenylalanine biosynthesis in Escherichia coli [J ] . Current Microbiology , 2001 , 43 ( 1 ): 26 - 32 .

YAKANDAWALA N , ROMEO T , FRIESEN A D , et al . Metabolic engineering of Escherichia coli to enhance phenylalanine production [J ] . Applied Microbiology and Biotechnology , 2008 , 78 ( 2 ): 283 - 291 .

TSOI R , WU F L , ZHANG C , et al . Metabolic division of labor in microbial systems [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2018 , 115 ( 10 ): 2526 - 2531 .

DINH C V , PRATHER K L J . Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineered Escherichia coli [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2019 , 116 ( 51 ): 25562 - 25568 .

WANG J , ZHANG R H , ZHANG Y , et al . Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production [J ] . Metabolic Engineering , 2019 , 55 ( 6 ): 191 - 200 .

YOUNGER A K , DALVIE N C , ROTTINGHAUS A G , et al . Engineering modular biosensors to confer metabolite-responsive regulation of transcription [J ] . ACS Synthetic Biology , 2017 , 6 ( 2 ): 311 - 325 .

RAMAN S , ROGERS J K , TAYLOR N D , et al . Evolution-guided optimization of biosynthetic pathways [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2014 , 111 ( 50 ): 17803 - 17808 .

SHI S B , ANG E L , ZHAO H M . In vivo biosensors: mechanisms, development, and applications [J ] . Journal of Industrial Microbiology & Biotechnology , 2018 , 45 ( 7 ): 491 - 516 .

FANG M Y , WANG T M , ZHANG C , et al . Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli [J ] . Metabolic Engineering , 2016 , 33 : 41 - 51 .

LIU Y F , ZHUANG Y Y , DING D Q , et al . Biosensor-based evolution and elucidation of a biosynthetic pathway in Escherichia coli [J ] . ACS Synthetic Biology , 2017 , 6 ( 5 ): 837 - 848 .

DING D Q , LI J L , BAI D Y , et al . Biosensor-based monitoring of the central metabolic pathway metabolites [J ] . Biosensors & Bioelectronics , 2020 , 167 : 112456 .

CHEN M , CHEN L , ZENG A P . CRISPR/Cas9-facilitated engineering with growth-coupled and sensor-guided in vivo screening of enzyme variants for a more efficient chorismate pathway in E. coli [J ] . Metabolic Engineering Communications , 2019 , 9 ( 2 ): e00094 .

陈胜杰 . L-色氨酸生产菌构建及发酵条件优化 [D ] . 天津 : 天津科技大学 , 2016 .

CHRN S J . Construction of L-tryptophan producing strain and optimization of fermentation conditions [D ] . TianJin : Tianjin University of Science and Technology , 2016 .

黄静 , 史建明 , 霍文婷 , 等 . 氮源对L-色氨酸发酵的影响 [J ] . 食品与发酵工业 , 2011 , 37 ( 5 ): 21 - 25 .

HUANG J , SHI J M , HUO W T , et al . The effects of nitrogen sources on the fermentation of L-tryptophan [J ] . Food and Fermentation Industries , 2011 , 37 ( 5 ): 21 - 25 .

杨梦晨 . L-色氨酸新型清液发酵工艺研究 [D ] . 天津 : 天津科技大学 , 2018 .

YANG M C . Study on fermentation and fermentation technology of L-tryptophan [D ] . Tianjin : Tianjin University of Science and Technology , 2018 .

娄秀平 . Escherichia coli JN8发酵产L-色氨酸的研究 [D ] . 无锡 : 江南大学 , 2014 .

LOU X P . Production of L-tryptophan by Escherichia coli JN8 [D ] . Wuxi : Jiangnan University , 2014 .

张婷婷 . 微生物法生产L-色氨酸的检测及发酵过程优化 [D ] . 开封 : 河南大学 , 2017 .

ZHANG T T . Determination of L-tryptophan grade in microbial production and optimization of fermentation process [D ] . Kaifeng : Henan University , 2017 .

PAREKH S , VINCI V A , STROBEL R J . Improvement of microbial strains and fermentation processes [J ] . Applied Microbiology and Biotechnology , 2000 , 54 ( 3 ): 287 - 301 .

JING K J , TANG Y W , YAO C Y , et al . Overproduction of L-tryptophan via simultaneous feed of glucose and anthranilic acid from recombinant Escherichia coli W3110: kinetic modeling and process scale-up [J ] . Biotechnology and Bioengineering , 2018 , 115 ( 2 ): 371 - 381 .

REIFENRATH M , BOLES E . Engineering o f hydroxymandelate synthases and the aromatic amino acid pathway enables de novo biosynthesis of mandelic and 4-hydroxymandelic acid with Saccharomyces cerevisiae [J ] . Metabolic Engineering , 2018 , 45 ( 2 ): 246 - 254 .

CAO M F , GAO M R , SUÁSTEGUI M , et al . Building microbial factories for the production of aromatic amino acid pathway derivatives: from commodity chemicals to plant-sourced natural products [J ] . Metabolic Engineering , 2020 , 58 : 94 - 132 .

FUJIWARA R , NODA S , TANAKA T , et al . Muconic acid production using gene-level fusion proteins in Escherichia coli [J ] . ACS Synthetic Biology , 2018 , 7 ( 11 ): 2698 - 2705 .

RAMAKRISHNA A , GIRIDHAR P , RAVISHANKAR G A . Phytoserotonin: a review [J ] . Plant Signaling & Behavior , 2011 , 6 ( 6 ): 80 - 92 .

DU J , YANG D , LUO Z W , et al . Metabolic engineering of Escherichia coli for the production of indirubin from glucose [J ] . Journal of Biotechnology , 2018 , 267 : 19 - 28 .

HAN G H , GIM G H , KIM W , et al . Enhanced indirubin production in recombinant Escherichia coli harboring a flavin-containing monooxygenase gene by cysteine supplementation [J ] . Journal of Biotechnology , 2012 , 164 ( 2 ): 179 - 187 .

RODRIGUEZ A , KILDEGAARD K R , LI M , et al . Establishment of a yeast platform strain for production of p -coumaric acid through metabolic engineering of aromatic amino acid biosynthesis [J ] . Metabolic Engineering , 2015 , 31 : 181 - 188 .

WEI T , CHENG B Y , LIU J Z . Genome engineering Escherichia coli for L-Dopa overproduction from glucose [J ] . Scientific Reports , 2016 , 6 : 30080 .

VALLIERE M A , KORMAN T P , WOODALL N B , et al . A cell-free platform for the prenylation of natural products and application to cannabinoid production [J ] . Nature Communications , 2019 , 10 ( 1 ): 565 .

LEE M Y , HUNG W P , TSAI S H . Improvement of shikimic acid production in Escherichia coli with growth phase-dependent regulation in the biosynthetic pathway from glycerol [J ] . World Journal of Microbiology and Biotechnology , 2017 , 33 ( 2 ): 1 - 8 .

SILVA D A , YU S , ULGE U Y , et al . De novo design of potent and selective mimics of IL-2 and IL-15 [J ] . Nature , 2019 , 565 ( 7738 ): 186 - 191 .

YANG X , YUAN Q Q , LUO H , et al . Systematic design and in vitro validation of novel one-carbon assimilation pathways [J ] . Metabolic Engineering , 2019 , 56 ( 1 ): 142 - 153 .

D'OELSNITZ S , ELLINGTON A . Continuous directed evolution for strain and protein engineering [J ] . Current Opinion in Biotechnology , 2018 , 53 : 158 - 163 .

BRYSON D I , FAN C , GUO L T , et al . Continuous directed evolution of aminoacyl-tRNA synthetases [J ] . Nature Chemical Biology , 2017 , 13 ( 12 ): 1253 - 1260 .

SONG L , ZENG A P . Engineering 'cell robots' for parallel and highly sensitive screening of biomolecules under in vivo conditions [J ] . Scientific Reports , 2017 , 7 ( 1 ): 15 - 45 .

BADRAN A H , LIU D R . Development of potent in vivo mutagenesis plasmids with broad mutational spectra [J ] . Nature Communications , 2015 , 6 : 8425 .

JAKOČIŪNAS T , PEDERSEN L E , LIS A V , et al . CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9 [J ] . Metabolic Engineering , 2018 , 48 ( 2 ): 88 - 96 .

HALPERIN S O , TOU C J , WONG E B , et al . CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window [J ] . Nature , 2018 , 560 ( 7717 ): 248 - 252 .

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