Research progress in 2-phenylethanol production through biological processes

YAN Wei; GAO Hao; JIANG Yujia; QIAN Xiujuan; ZHOU Jie; DONG Weiliang; ZHANG Wenming; XIN Fengxue; JIANG Min

doi:10.12211/2096-8280.2020-096

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Research progress in 2-phenylethanol production through biological processes

Invited Review | 更新时间：2025-03-20

- Research progress in 2-phenylethanol production through biological processes
- Synthetic Biology Journal Vol. 2, Issue 6, Pages: 1030-1045(2021)
- 作者机构：
  
  1.南京工业大学生物与制药工程学院，材料化学工程国家重点实验室，江苏南京 211816
  2.南京工业大学江苏先进生物与化学制造协同创新中心（SICAM），江苏南京 211816
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2020-096
  CLC： Q81
- Received：31 December 2020，
  
  Revised：2021-03-10，
  
  Published：31 December 2021
- 稿件说明：
移动端阅览
严伟，高豪，蒋羽佳，钱秀娟，周杰，董维亮，章文明，信丰学，姜岷. 2-苯乙醇生物合成的研究进展［J］. 合成生物学， 2021， 2（6）： 1030-1045

YAN Wei， GAO Hao， JIANG Yujia， QIAN Xiujuan， ZHOU Jie， DONG Weiliang， ZHANG Wenming， XIN Fengxue， JIANG Min. Research progress in 2-phenylethanol production through biological processes［J］. Synthetic Biology Journal， 2021， 2（6）： 1030-1045
严伟，高豪，蒋羽佳，钱秀娟，周杰，董维亮，章文明，信丰学，姜岷. 2-苯乙醇生物合成的研究进展［J］. 合成生物学， 2021， 2（6）： 1030-1045 DOI： 10.12211/2096-8280.2020-096.

YAN Wei， GAO Hao， JIANG Yujia， QIAN Xiujuan， ZHOU Jie， DONG Weiliang， ZHANG Wenming， XIN Fengxue， JIANG Min. Research progress in 2-phenylethanol production through biological processes［J］. Synthetic Biology Journal， 2021， 2（6）： 1030-1045 DOI： 10.12211/2096-8280.2020-096.

摘要

2-苯乙醇（2-PE）是一种具有玫瑰香味的重要香料化合物，广泛应用于化妆品、香水、食品等行业。传统的2-PE生产主要是从植物原料中提取或化学合成。然而，这些方法无法满足消费者对天然香料日益增长的需求。以发酵法或酶法生产的L-苯丙氨酸为前体，利用酵母细胞将其转化为2-PE，产品既符合环境友好的要求，又满足“天然”产品的定义，可以取代从玫瑰或其他植物精油中提取的天然2-PE。因此，生物法合成2-PE已经引起人们的广泛关注。本文综述了生物法合成2-PE的现状和发展前景，指出相对于传统的化学合成和天然植物提取，生物转化比较具有优越性。并对2-PE的合成途径、全局调控机制、提高2-PE产量的策略以及农工废弃物作为原料的利用进行了系统的论述。此外，本文还讨论了原位产物分离技术在2-PE生物合成中的应用。

Abstract

2-Phenylethanol (2-PE)

an important flavor and fragrance compound with a rose-like smell has been widely used in the cosmetics

perfume

and food industries. Furthermore

it is also an important raw material for the derivatives synthesis for the synthesis of other flavors or pharmaceutical compounds

such as 2-phenylethylacetate (2-PEAc) and phenylacetaldehyde (PA). Conventional production of 2-PE is mainly through the extraction from plant materials

such as hyacinths

jasmine and lilies. However

the extraction proce

ss is very complicated and the harvest of flowers is greatly influenced by the weather

plant diseases

and trade restrictions

which hinders its further application. On the other hand

2-PE can also be chemically synthesized through ethylene oxidation of benzene or reduction of styrene oxide. Chemical synthesis processes are generally operated under harsh conditions

such as high temperature

high pressure

and strong acid or alkali environments

producing many undesirable by-products

such as ethylbenzene and styrene. This not only increases the downstream costs

but also seriously debases the grade of 2-PE. The increasing demand for environmental friendly processes and the preference for “natural” products from consumers have considerably stimulated the development of biological production of flavors and fragrances. Biological synthesis of 2-PE has attracted extensive attention because it meets the requirements of environmental friendliness and also satisfies the definition of “natural” products. However

the toxicity of 2-PE to cells is a critical limiting factor for the biosynthesis of 2-PE. In this review

we have comprehensively summarized the current status and perspectives for biological 2-PE production in terms of its advantages over classical chemical synthesis and extraction from natural plants. A comprehensive description of 2-PE synthetic pathways and global regulation mechanisms

strategies to increase 2-PE production

and the utilization of agro-industrial wastes as feedstocks has been systematically discussed. Furthermore

the application of

in situ

product removal techniques in 2-PE biosynthesis has also been highlighted.

关键词

Keywords

references

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Related Author

Wei Yan

Hao Gao

Yujia Jiang

Xiujuan Qian

Jie Zhou

Weiliang Dong

Wenming Zhang

Fengxue Xin

Related Institution

State Key Laboratory of Materials-Oriented Chemical Engineering， College of Biotechnology and Pharmaceutical Engineering， Nanjing Tech University

Jiangsu National Synergetic Innovation Center for Advanced Materials （SICAM）， Nanjing Tech University

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety， CAS Center for Excellence in Nanoscience， National Center for Nanoscience and Technology of China

School of Nanoscience and Engineering， University of Chinese Academy of Sciences

College of Materials Science and Opto-Electronic Technology， University of Chinese Academy of Sciences

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