Biological synthesis and applications of artificial protein functional materials

ZENG Dan; CHU Jianlin; CHEN Yanru; FAN Daidi

doi:10.12211/2096-8280.2020-091

您当前的位置：

首页 >

文章列表页 >

Biological synthesis and applications of artificial protein functional materials

Invited Review | 更新时间：2026-03-05

- Biological synthesis and applications of artificial protein functional materials
- Synthetic Biology Journal Vol. 2, Issue 4, Pages: 528-542(2021)
- 作者机构：
  
  1.西北大学化工学院，陕西省可降解生物医用材料重点实验室，陕西省生物材料与发酵工程技术研究中心，陕西西安 710069
  2.南京工业大学药学院，江苏南京 210000
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2020-091
  CLC： Q816
- Received：28 December 2020，
  
  Revised：2021-05-29，
  
  Published：31 August 2021
- 稿件说明：
移动端阅览
曾丹，储建林，陈燕茹，范代娣. 人造蛋白功能材料的生物合成及应用［J］. 合成生物学， 2021， 2（4）： 528-542

ZENG Dan， CHU Jianlin， CHEN Yanru， FAN Daidi. Biological synthesis and applications of artificial protein functional materials［J］. Synthetic Biology Journal， 2021， 2（4）： 528-542
曾丹，储建林，陈燕茹，范代娣. 人造蛋白功能材料的生物合成及应用［J］. 合成生物学， 2021， 2（4）： 528-542 DOI： 10.12211/2096-8280.2020-091.

ZENG Dan， CHU Jianlin， CHEN Yanru， FAN Daidi. Biological synthesis and applications of artificial protein functional materials［J］. Synthetic Biology Journal， 2021， 2（4）： 528-542 DOI： 10.12211/2096-8280.2020-091.

摘要

人造蛋白功能材料具有良好的生物相容性和生物可降解性，来源广泛且功能多样，是一类理想的生物材料，在生物工程、医药、军事和纺织等领域具有广泛的应用前景。然而，现阶段蛋白功能材料的微生物合成仍存在表达量低、性能不稳定等问题，严重限制了这些蛋白材料的高效生产与应用。合成生物学在工程化理念的指导下，为人造蛋白功能材料提供了“精准设计—系统构建—调控表达—工程应用”的研究策略。本文介绍了蛛丝蛋白、蚕丝蛋白、类人胶原蛋白和贻贝蛋白等主要蛋白功能材料的生物合成研究进展，并阐述了人造蛋白细胞工厂的构建、蛋白的调控表达和其在组织工程材料等领域的应用现状。具有动态响应特性的人造蛋白功能材料是未来的研究方向。

Abstract

Artificial protein functional materials have good biocompatibility

biodegradability

broad resources of raw materials

and diverse functions. As ideal biological materials

they have a wide range of application prospects in bioengineering

medicine

military and textiles. However

microbial synthesis of protein functional materials still has problems such as low expression and unstable performance

which severely restrict their efficient production and application. In recent years

there is a trend of designing artificial protein functional materials that not only possess multi-functional properties but are biomimetic

adaptive and dynamically responsive to biological processes

and such a trend puts forward new requirements for the design and engineering control of macromolecular systems

which raise a necessity for developing research methods to assemble engineered protein molecules into functional macroscopic materials. Under the guidance of engineering concepts

synthetic biology provides a strategy for developing "precise design-system construction-regulatory expres

sion-engineering application" for artificial protein functional materials. This review comments the research progress in the biological synthesis of major protein functional materials such as spider silk protein

silk protein

human-like collagen and mussel protein

and explores the construction of artificial protein cell factory

the regulation of protein expression and their applications as tissue engineering materials and other fields. In view of the current problems of poor biocompatibility and insufficient efficacy of clinical products

artificial protein functional materials can be assembled and processed according to functional requirements

and used to fabricate artificial tendons

artificial skin

degradable hemostatic materials

artificial bones and new protein material medical products such as high-viscosity anti-fouling coating products. There are still several challenges that need to be solved urgently: (1) There are few theoretical analysis tools and models at this stage

making the design

prediction and analysis of functional proteins relatively limited

and a more comprehensive protein database needs to be established; (2) There are many expression regulators in

E. coli

and other microbial systems

and adaptation for the design of expression elements and the target gene

the regulation of protein synthesis pathways and the systemic nature need to be studied to improve the efficiency of protein expression; (3) In terms of engineering applications

it is also necessary to comprehensively consider material stability and biological safety. Finally

the research direction is prospected

which provides insights for scholars working in related fields.

关键词

Keywords

references

李春 . 合成生物学 [M ] . 北京 : 化学工业出版社 , 2019 : 9 .

LI C . Synthetic biology [M ] . Beijing : Chemical Industry Press , 2019 : 9 .

ENDY D . Foundations for engineering biology [J ] . Nature , 2005 , 438 ( 7067 ): 449 - 453 .

KHALIL A S , COLLINS J J . Synthetic biology: applications come of age [J ] . Nature Reviews Genetics , 2010 , 11 ( 5 ): 367 - 379 .

GIROTTI A , FERNáNDEZ-COLINO A , LÓPEZ I M , et al . Elastin-like recombinamers: biosynthetic strategies and biotechnological applications [J ] . Biotechnology Journal , 2011 , 6 ( 10 ): 1174 - 1186 .

YIGIT S , DINJASKI N , KAPLAN D L . Fibrous proteins: at the crossroads of genetic engineering and biotechnological applications [J ] . Biotechnology and Bioengineering , 2016 , 113 ( 5 ): 913 - 929 .

DESAI M S , LEE S-W . Protein-based functional nanomaterial design for bioengineering applications [J ] . WIREs Nanomedicine and Nanotechnology , 2015 , 7 ( 1 ): 69 - 97 .

CHANG Y , JIAO Y , SYMONS H E , et al . Molecular engineering of polymeric supra-amphiphiles [J ] . Chemical Society Reviews , 2019 , 48 ( 4 ): 989 - 1003 .

SUN J , SU J , MA C , et al . Fabrication and mechanical properties of engineered protein-based adhesives and fibers [J ] . Advanced Materials , 2020 , 32 ( 6 ): e1906360 .

HAGN F , EISOLDT L , HARDY J G , et al . A conserved spider silk domain acts as a molecular switch that controls fibre assembly [J ] . Nature , 2010 , 465 : 239 - 242 .

ADACHI T , WANG X , MURATA T , et al . Production of a non-triple helical collagen alpha chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture [J ] . Biotechnology and Bioengineering , 2010 , 106 ( 6 ): 860 - 870 .

HE J , MA X , ZHANG F , et al . New strategy for expression of recombinant hydroxylated human collagen alpha1(Ⅲ) chains in Pichia pastoris GS115 [J ] . Biotechnology and Applied Biochemistry , 2015 , 62 ( 3 ): 293 - 299 .

CHRISTOPH R , STEPHAN B , JÜRG C , et al . Recombinant expression of hydroxylated human collagen in Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 10 ): 4445 - 4455 .

RISING A , JOHANSSON J . Toward spinning artificial spider silk [J ] . Nature Chemical Biology , 2015 , 11 ( 5 ): 309 - 315 .

XU J , WANG L N , ZHU C H , et al . Co-expression of recombinant human prolyl with human collagen alpha1 (Ⅲ) chains in two yeast systems [J ] . Letters in Applied Microbiology , 2015 , 61 ( 3 ): 259 - 266 .

TANG Y , YANG X , HANG B , et al . Efficient production of hydroxylated human-like collagen via the co-expression of three key genes in Escherichia coli origami (DE3) [J ] . Applied Biochemistry and Biotechnology , 2016 , 178 ( 7 ): 1458 - 1470 .

WANG Y , KATYAL P , MONTCLARE J K . Protein-engineered functional materials [J ] . Advanced Healthcare Materials , 2019 , 8 ( 11 ): 1801374 .

HUANG P S , BOYKEN S E , BAKER D . The coming of age of de novo protein design [J ] . Nature , 2016 , 537 ( 7620 ): 320 - 327 .

LANGAN R A , BOYKEN S E , NG A H , et al . De novo design of bioactive protein switches [J ] . Nature , 2019 , 572 ( 7768 ): 205 - 210 .

ALM E , BAKER D . Prediction of protein-folding mechanisms from free-energy landscapes derived from native structures [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1999 , 96 ( 20 ): 11305 - 11310 .

LAI Y T , READING E , HURA G L , et al . Structure of a designed protein cage that self-assembles into a highly porous cube [J ] . Nature Chemistry , 2014 , 6 ( 12 ): 1065 - 1071 .

LIN S , RYU S , TOKAREVA O , et al . Predictive modelling-based design and experiments for synthesis and spinning of bioinspired silk fibres [J ] . Nature Communications , 2015 , 6 ( 1 ): 1 - 12 .

LI Y , LI J , SUN J , et al . Bio-inspired and mechanically strong fibers based on engineered non-spider chimeric proteins [J ] . Angewandte Chemie International Edition , 2020 , 59 ( 21 ): 8148 - 8152 .

EISOLDT L , SMITH A , SCHEIBEL T . Decoding the secrets of spider silk [J ] . Materials Today , 2011 , 14 ( 3 ): 80 - 86 .

YARGER J L , CHERRY B R , VAN DER VAART A . Uncovering the structure-function relationship in spider silk [J ] . Nature Reviews Materials , 2018 , 3 ( 3 ): 18008 .

ALLEY E C , KHIMULYA G , BISWAS S , et al . Unified rational protein engineering with sequence-based deep representation learning [J ] . Nature Methods , 2019 , 16 ( 12 ): 1315 - 1322 .

YANG K K , WU Z , ARNOLD F H . Machine-learning-guided directed evolution for protein engineering [J ] . Nature Methods , 2019 , 16 ( 8 ): 687 - 694 .

NG A H , NGUYEN T H , GOMEZ-SCHIAVON M , et al . Modular and tunable biological feedback control using a de novo protein switch [J ] . Nature , 2019 , 572 ( 7768 ): 265 - 269 .

刘凯 , 李敬敬 , 马俊 , 等 . 力学功能蛋白生物合成及材料应用 [J ] . 高分子学报 , 2020 , 51 : 698 - 709 .

LIU K , LI J J , MA J , et al . Biological synthesis of structural proteins and applications [J ] . Acta Polymerica Sinica , 2020 , 51 : 698 - 709 .

ZHENG K , LING S . De novo design of recombinant spider silk proteins for material applications [J ] . Biotechnology Journal , 2019 , 14 ( 1 ): 1700753 .

FRANCO A R , FERNANDES E M , MARCIA T R , et al . Antimicrobial coating of spider silk to prevent bacterial attachment on silk surgical sutures [J ] . Acta Biomaterialia , 2019 , 99 : 236 - 246 .

SALEHI S , KOECK K , SCHEIBEL T . Spider silk for tissue engineering applications [J ] . Molecules , 2020 , 25 ( 3 ): 737 .

SCHELLER J , GÜHRS K-H , GROSSE F , et al . Production of spider silk proteins in tobacco and potato [J ] . Nature Biotechnology , 2001 , 19 ( 6 ): 573 - 577 .

LAZARIS A , ARCIDIACONO S , HUANG Y , et al . Spider silk fibers spun from soluble recombinant silk produced in mammalian cells [J ] . Science , 2002 , 295 ( 5554 ): 472 - 476 .

FOO C W P , BINI E , HENSMAN J , et al . Role of pH and charge on silk protein assembly in insects and spiders [J ] . Applied Physics A , 2006 , 82 ( 2 ): 223 - 233 .

LIU B , WANG T , XIAO L , et al . A directed self-assembly quasi-spider silk protein expressed in Pichia pastoris [J ] . Biotechnology & Biotechnological Equipment , 2018 , 32 ( 2 ): 451 - 461 .

ARCIDIACONO S , MELLO C , KAPLAN D , et al . Purification and characterization of recombinant spider silk expressed in Escherichia coli [J ] . Applied Microbiology and Biotechnology , 1998 , 49 ( 1 ): 31 - 38 .

XIA X X , QIAN Z G , KI C S , et al . Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2010 , 107 ( 32 ): 14059 - 14063 .

ANDERSSON M , JIA Q , ABELLA A , et al . Biomimetic spinning of artificial spider silk from a chimeric minispidroin [J ] . Nature Chemical Biology , 2017 , 13 ( 3 ): 262 - 264 .

BOWEN C H , DAI B , SARGENT C J , et al . Recombinant spidroins fully replicate primary mechanical properties of natural spider silk [J ] . Biomacromolecules , 2018 , 19 ( 9 ): 3853 - 3860 .

KOH L D , YEO J , LEE Y Y , et al . Advancing the frontiers of silk fibroin protein-based materials for futuristic electronics and clinical wound-healing (invited review) [J ] . Materials Science and Engineering: C, 2018 , 86: 151 - 172 .

VEPARI C , KAPLAN D L . Silk as a biomaterial [J ] . Progress in Polymer Science , 2007 , 32 ( 8/9 ): 991 - 1007 .

PANDEY V , HAIDER T , JAIN P , et al . Silk as a leading-edge biological macromolecule for improved drug delivery [J ] . Journal of Drug Delivery Science Technology , 2019 , 55 : 101294 .

SINGH M , BOLLELLA P , GORTON L , et al . Conductive and enzyme-like silk fibers for soft sensing application [J ] . Biosensors & Bioelectronics , 2019 , 150 : 111859 .

ANDERSSON M , JOHANSSON J , RISING A . Silk spinning in silkworms and spiders [J ] . International Journal of Molecular Sciences , 2016 , 17 ( 8 ): 1290 .

XU J , DONG Q , YU Y , et al . Mass spider silk production through targeted gene replacement in Bombyx mori [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2018 , 115 ( 35 ): 8757 - 8762 .

ZHANG X , XIA L , DAY B A , et al . CRISPR/Cas9 initiated transgenic silkworms as a natural spinner of spider silk [J ] . Biomacromolecules , 2019 , 20 ( 6 ): 2252 - 2264 .

LU T K , KHALIL A S , COLLINS J J . Next-generation synthetic gene networks [J ] . Nature Biotechnology , 2009 , 27 ( 12 ): 1139 .

WAN X , PINTO F , YU L , et al . Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity [J ] . Nature Communications , 2020 , 11 : 5961 .

SHI J , MA X , GAO Y , et al . Hydroxylation of human type Ⅲ collagen alpha chain by recombinant coexpression with a viral prolyl 4-hydroxylase in Escherichia coli [J ] . The Protein Journal , 2017 , 36 : 322 - 331 .

XU R , LUO Y-E , FAN D-D , et al . Improving the production of human-like collagen by pulse-feeding glucose during the fed-batch culture of recombinant Escherichia coli [J ] . Biotechnology Applied Biochemistry , 2012 , 59 ( 5 ): 330 - 337 .

FAN D D , LUO Y , MI Y , et al . Characteristics of fed-bat ch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates [J ] . Biotechnology Letters , 2005 , 27 ( 12 ): 865 - 870 .

CHEN Z Y , FAN D D , SHANG L J . Exploring the potential of the recombinant human collagens for biomedical and clinical applications: a short review [J ] . Biomedical Materials , 2020 , 16 ( 1 ): 012001 .

YANG B , AYYADURAI N , YUN H , et al . In vivo residue-specific dopa-incorporated engineered mussel bioglue with enhanced adhesion and water resistance [J ] . Angewandte Chemie International Edition , 2014 , 53 : 1 3360 – 13364 .

SPOHNER S C , MÜLLER H , QUITMANN H , et al . Expression of enzymes for the usage in food and feed industry with Pichia pastoris [J ] . Journal of Biotechnology , 2015 , 202 : 118 - 134 .

ZHU J , GUO M , ZHUANG Y , et al . Eﬃcient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastoris [J ] . Journal of Applied Microbiology , 2009 , 107 : 954 - 963 .

YANG J , LU Z , CHEN J , et al . Efficient of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris [J ] . Applied Microbiology & Biotechnology , 2016 , 100 : 5453 - 5465 .

PAN R , ZHANG J , SHEN W-L , et al . Sequential deletion of Pichia pastoris genes by a self-excisable cassette [J ] . Federation of European Microbiological Societies Yeast Research , 2011 , 11 ( 3 ): 292 - 298 .

VERMA M , CHOI J , COTTRELL K A , et al . A short translational ramp determines the efficiency of protein synthesis [J ] . Nature Communications , 2019 , 10 : 5774 .

李佳 , 王软林 , 梁爱华 . 真核翻译起始因子5A在蛋白质合成中的功能与机制 [J ] . 中国生物化学与分子生物学报 , 2021 , 37 ( 2 ): 161 - 168 .

LI J , WANG R L , LIANG A H . Function and mechanism of eukaryotic translation initiation factor 5A in protein synthesis [J ] . Chinese Journal of Biochemistry and Molecular Biology , 2021 , 37 ( 2 ): 161 - 168 .

JIA Q L , LUO Y E , FAN D D . Application of molecular chaperone to increase the expression of soluble human-like collagen in Escherichia coli [J ] . Biotechnology an Indian Journal , 2013 , 7 ( 12 ): 531 - 536 .

JIA Q L , LUO Y E , FAN D D , et al . The different roles of chaperone teams on over-expression of human-like collagen in recombinant Escherichia coli [J ] . Journal of the Taiwan Institute of Chemical Engineers , 2014 , 45 ( 6 ): 2843 - 2850 .

WILDT S , GERNGROSS T U . The humanization of N -glycosylation pathways in yeast [J ] . Nature Reviews Microbiology , 2005 , 3 ( 2 ): 119 - 128 .

ZHU B , SHEN J , ZHAO T , et al . Intact glycopeptide analysis of influenza A/H1N1/09 neuraminidase revealing the effects of host and glycosite location on site-specific glycan structures [J ] . Proteomics , 2019 , 19 ( 3 ): 1800202 .

BERN M , KIL Y J , BECKER C . Byonic: advanced peptide and protein identification software [J ] . Current Protocols in Bioinformatics , 2012 , 40 ( 1 ): 13.20.1 - 13.20.14 .

ESHGHI S T , SHAH P , YANG W M , et al . GPQuest: a spectral library matching algorithm for site-specific assignment of tandem mass spectra to intact N -glycopeptides [J ] . Analytical Chemistry , 2015 , 87 ( 10 ): 5181 - 5188 .

LIU M Q , ZENG W F , FANG P , et al . pGlyco 2.0 enables precision N -glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification [J ] . Nature Communications , 2017 , 8 ( 1 ): 438 .

XIAO K , TIAN Z . GPSeeker enables quantitative structural N -glycoproteomics for site-and structure-specific characterization of differentially expressed N -glycosylation in hepatocellular carcinoma [J ] . Journal of Proteome Research , 2019 , 18 ( 7 ): 2885 - 2895 .

POLASKY D A , YU F , TEO G C , et al . Fast and comprehensive N - and O -glycoproteomics analysis with MSFragger-Glyco [J ] . Nature Methods , 2020 , 17 ( 11 ): 1125 - 1132 .

BELDJILALI-LABRO M , GARCIA A G , FARHAT F , et al . Biomaterials in tendon and skeletal muscle tissue engineering: current trends and challenges [J ] . Materials , 2018 , 11 ( 7 ): 1116 .

JIANG J , ZHANG S , QIAN Z , et al . Protein bricks: 2D and 3D Bio-nanostructures with shape and function on demand [J ] . Advanced Materials , 2018 , 30 ( 20 ): 1705919 .

ZHI Y , LIU W , ZHANG P , et al . Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model [J ] . Biotechnology Letters , 2016 , 38 ( 10 ): 1827 - 1835 .

NAGHASHZARGAR E , FARÈ S , CATTO V , et al . Nano/Micro hybrid scaffold of PCL or P3HB nanofibers combined with silk fibroin for tendon and ligament tissue engineering [J ] . Journal of Applied Biomaterials & Functional Materials , 2015 , 13 ( 2 ): 156 - 168 .

TELLADO S T , BONANI W , BALMAYOR E R , et al . Fabrication and characterization of biphasic silk fibroin scaffolds for tendon/ligament-to-bone tissue engineering [J ] . Tissue Engineering Part A , 2017 , 23 ( 15-16 ): 859 - 872 .

WANG C , XIA K , ZHANG Y , et al . Silk-based advanced materials for soft electronics [J ] . Accounts of Chemical Research , 2019 , 52 ( 10 ): 2916 - 2927 .

GAO B , GUO M , LYU K , et al . Intelligent silk fibroin based microneedle dressing (i-SMD) [J ] . Advanced Functional Materials , 2021 , 31 ( 3 ): 2006839 .

GHOLIPOURMALEKABADI M , SAMADIKUCHAKSARAEI A , SEIFALIAN A M , et al . Silk fibroin/amniotic membrane 3D bi-layered artificial skin [J ] . Biomedical Materials , 2018 , 13 ( 3 ): 035003 .

KEIROUZ A , ZAKHAROVA M , KWON J , et al . High-throughput production of silk fibroin-based electrospun fibers as biomaterial for skin tissue engineering applications [J ] . Materials Science and Engineering: C, 2020 , 112: 110939 .

LI X , FAN D , ZHU C , et al . Effects of self-assembled fibers on the synthesis, characteristics and biomedical applications of CCAG hydrogels [J ] . Journal of Materials Chemistry B , 2014 , 2 ( 9 ): 1234 - 1249 .

LI X , XUE W , LIU Y , et al . Novel multifunctional PB and PBH hydrogels as soft filler for tissue engineering [J ] . Journal of Materials Chemistry B , 2015 , 3 ( 23 ): 4742 - 4755 .

JIANG X , WANG Y , FAN D , et al . A novel human-like collagen hemostatic sponge with uniform morphology, good biodegradability and biocompatibility [J ] . Journal of Biomaterials Applications , 2017 , 31 ( 8 ): 1099 - 1107 .

范代娣 , 马晓轩 , 米钰 , 等 . 一种可生物降解止血海绵材料及其制备方法 : CN200610041913.3 [P ] . 2006-08-23 .

FAN D D , MA X X , MI Y , et al . A biodegradable hemostatic sponge material and its preparation method : CN200610041913.3 [P ] . 2006-08-23 .

PAN H , FAN D D , CAO W , et al . Preparation and characterization of breathable hemostatic hydrogel dressings and determination of their effects on full-thickness defects [J ] . Polymers , 2017 , 9 ( 12 ): 727 .

FAN H , MI Y , HUI J , et al . Cytocompatibility of human-like collagen/nano-hydroxyapatite porous scaffolds using cartilages [J ] . Biotechnology , 2013 , 12 ( 2 ): 99 - 103 .

JIA L P , DUAN Z G , FAN D D , et al . Human-like collagen/nano-hydroxyapatite scaffolds for the culture of chondrocytes [J ] . Materials Science and Engineering C , 2013 , 33 ( 2 ): 727 - 734 .

LIU K Q , LIU Y N , DUAN Z G , et al . A biomimetic bi-layered tissue engineering scaffolds for osteochondral defects repair [J ] . Science China Technological Sciences , 2020 , 64 ( 4 ): 793 - 805 .

SONG X , ZHU C H , FAN D D , et al . A novel human-like collagen hydrogel scaffold with porous structure and sponge-like properties [J ] . Polymers , 2017 , 9 ( 12 ): 638 .

LIU Y N , GU J , FAN D D . Fabrication of high-strength and porous hybrid scaffolds based on nano-hydroxyapatite and human-like collagen for bone tissue regeneration [J ] . Polymers , 2020 , 12 ( 1 ): 61 .

XIE J H , FAN D D . A high-toughness and high cell adhesion polyvinyl alcohol (PVA-hyaluronic acid (HA)-human-like collagen (HLC) composite hydrogel for cartilage repair [J ] . International Journal of Polymeric Materials and Polymeric Biomaterials , 2020 , 69 ( 14 ): 928 - 937 .

ZHOU J , GUO X , ZHENG Q , et al . Improving osteogenesis of three-dimensional porous scaffold based on mineralized recombinant human-like collagen via mussel-inspired polydopamine and effective immobilization of BMP-2-derived peptide [J ] . Colloids and Surfaces B: Biointerfaces , 2017 , 152 : 124 - 132 .

DAMODARAN V B , MURTHY N S . Bio-inspired strategies for designing antifouling biomaterials [J ] . Biomaterials Research , 2016 , 20 : 18 .

吕亚维 , 王睿劼 , 张雨靖等 . 重组贻贝黏蛋白 Mgfp-5 的表达及功能评价 [J ] . 基因组学与应用生物学 , 2017 , 36 ( 10 ): 4108 - 4115 .

LYU Y W , WANG R J , ZHANG Y J , et al . The expression and function evaluation of the recombinated mussel adhesive protein Mgfp-5 [J ] . Genomics and Applied Biology , 2017 , 36 ( 10 ): 4108 - 4115 .

薛瑞 , 姚林 , 王瑞 , 等 . 重组贻贝足蛋白的研究进展与应用 [J ] . 中国生物工程杂志 , 2020 , 40 ( 11 ): 82 - 89 .

XUE R , YAO L , WANG R , et al . Advances and applications of recombinant mussel foot proteins [J ] . China Biotechnology , 2020 , 40 ( 11 ): 82 - 89 .

QI H S , ZHENG W W , ZHOU X , et al . A mussel-inspired chimeric protein as a novel facile antifouling coating [J ] . Chemical Communications , 2018 , 54 : 11328 - 11331 .

QI H S , ZHENG W W , ZHANG C , et al . Novel mussel-inspired universal surface functionalization strategy: protein-based coating with residue-specific post-translational modification in vivo [J ] . American Chemical Society Applied Materials & Interfaces , 2019 , 11 ( 13 ): 12846 - 12853 .

QI H S , ZHANG C , GUO H S , et al . Bioinspired multifunctional protein coating for antifogging, self-cleaning, and antimicrobial properties [J ] . American Chemical Society Applied Materials & Interfaces , 2019 , 11 ( 27 ): 24504 - 24511 .

WANG J X , CAO H L , ZHANG J Z H , et al . Computational protein design with deep learning neural networks [J ] . Scientific Reports , 2018 , 8 ( 1 ): 6349 - 6358 .

胡如云 , 张嵩亚 , 蒙海林 , 等 . 面向合成生物学的机器学习方法及应用 [J ] . 科学通报 , 2021 , 66 ( 3 ): 284 - 299 .

HU R Y , ZHANG S Y , MENG H L , et al . Machine learning for synthetic biology: methods and applications [J ] . Chinese Science Bulletin , 2021 , 66 ( 3 ): 284 - 299 .

STAPLES M , CHAN L , SI D , et al . Artificial intelligence for bioinformatics: applications in protein folding prediction [C ] // Proceedings of the 2019 IEEE Technology & Engineering Management Conference (TEMSCON) , Atlanta, GA, USA : 2019 .

DRIENOVSKá I , ROELFES G . Expanding the enzyme universe with genetically encoded unnatural amino acids [J ] . Nature Catalysis , 2020 , 3 : 193 - 202 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Synthetic biology drives the sustainable production of terpenoid fragrances and flavors

Advances and applications of single-cell Raman spectroscopy testing and sorting equipment

Rewiring and application of Yarrowia lipolytica chassis cell

Advances with applications of Raman spectroscopy in single-cell phenotype sorting and analysis

Related Author

Dan ZENG

Jianlin CHU

Yanru CHEN

Daidi FAN

ZHANG Mengyao

CAI Peng

ZHOU Yongjin

DIAO Zhidian

Related Institution

School of Chemical Engineering， Shaanxi Key Laboratory of Degradable Biomedical Materials， Shaanxi R&D Center of Biomaterials and Fermentation Engineering， Northwest University， Taibai North Road

School of Pharmacy， Nanjing University of Technology， Puzhu South Road 30

Division of Biotechnology， Dalian Institute of Chemical Physics， Chinese Academy of Sciences

Dalian Key Laboratory of Energy Biotechnology

University of Chinese Academy of Sciences

⁰