Enzymatic synthesis of 2<italic style="font-style: italic">'</italic>-fucosyllactose： advances and perspectives

SHI Ran; JIANG Zhengqiang

doi:10.12211/2096-8280.2020-033

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Enzymatic synthesis of 2'-fucosyllactose： advances and perspectives

Invited Review | 更新时间：2025-03-20

- Enzymatic synthesis of 2'-fucosyllactose： advances and perspectives
- Synthetic Biology Journal Vol. 1, Issue 4, Pages: 481-494(2020)
- 作者机构：
  
  中国农业大学食品科学与营养工程学院，北京 100083
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2020-033
  CLC： Q814.9
- Received：23 March 2020，
  
  Revised：2020-05-09，
  
  Published：31 August 2020
- 稿件说明：
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史然, 江正强. 2'-岩藻糖基乳糖的酶法合成研究进展和展望[J]. 合成生物学, 2020, 1(4): 481-494

SHI Ran, JIANG Zhengqiang. Enzymatic synthesis of 2'-fucosyllactose: advances and perspectives[J]. Synthetic Biology Journal, 2020, 1(4): 481-494
史然, 江正强. 2'-岩藻糖基乳糖的酶法合成研究进展和展望[J]. 合成生物学, 2020, 1(4): 481-494 DOI： 10.12211/2096-8280.2020-033.

SHI Ran, JIANG Zhengqiang. Enzymatic synthesis of 2'-fucosyllactose: advances and perspectives[J]. Synthetic Biology Journal, 2020, 1(4): 481-494 DOI： 10.12211/2096-8280.2020-033.

摘要

人乳寡糖（human milk oligosaccharides，HMOs）是人乳中一类结构复杂、非消化性的碳水化合物。2

岩藻糖基乳糖（2

-fucosyllactose，2

-FL）是人乳中含量最高的寡糖，也是最早被FDA和欧盟批准可添加到婴幼儿奶粉、膳食补充剂以及医疗食品中的HMOs之一。2

-FL具有调节肠道菌群、抵抗病原菌的黏附、免疫调节及促进神经系统发育和修复等多种功能活性。2

-FL的主要合成方法有化学合成法、全细胞合成法及酶催化合成法。全细胞合成法是当前工业上生产2

-FL的主要方法，降低L-岩藻糖的成本、调节合成途径中鸟苷二磷酸-L-岩藻糖（GDP-岩藻糖）的水平与菌体生长之间的平衡、发掘新型高活性的

-1，2-岩藻糖基转移酶是降低全细胞合成2

-FL成本的关键。2

-FL的合成途径在一些更为安全表达宿主（如无抗生素大肠杆菌、枯草芽孢杆菌和酵母菌等）中的构建也面临着挑战。本文重点综述了2

-FL酶法合成的研究现状，利用

-1，2-岩藻糖基转移酶合成2

-FL专一性好，但所需糖基供体GDP-岩藻糖成本较高；利用

-L-岩藻糖苷酶的转糖苷活性也可以合成2

-FL，

-L-岩藻糖苷酶来源广泛，易获得，稳定性好，可利用天然底物作为糖基供体。将

-L-岩藻糖苷酶应用于2

-FL合成的关键在于高效的转糖苷酶以及天然、经济的岩藻糖基供体的发掘。酶法合成将来有望成为工业上生产2

-FL的方法。

Abstract

Human milk oligosaccharides (HMOs) constitute a unique group of endogenous indigestible carbohydrates in human breast milk. HMOs play a crucial role in infant health and growth. As the most abundant HMO

2'-fucosyllactose (Fucα1

2Galβ1

4Glc

2'-FL) has been approved for infant formulas

dietary supplements and medical foods in the United States and European Union. 2'-FL has been synthesized by chemical

enzymatic synthesis and cell factory approaches

and currently mainly produced by cell factory approach. The crucial factors for 2'-FL production are the reduction of the cost of L-fucose

the discovery of novel α1

2-fucosyltransferases

and the maintenance of the balance between intracellular GDP-L-fucose level and the growth of engineering strain. Also

the construction of antibiotic-free system (such as antibiotic-free

Escherichia coli

Bacillus subtilis

and

Saccharomyces cerevisiae

) is still a challenge for the synthesis of 2'-FL. In this review

the research progress of enzymatic synthesis of 2'-FL was particularly presented. 2'-FL could be enzymatically synthesized using α-1

2-fucosyltransferases or α-L-fucosidases. α-1

2-Fucosyltransferases catalyze the transformation of a fucose from a GDP-L-fucose to a lactose. The main disadvantage for 2'-FL synthesis by fucosyltransferase is the requirement for an expensive nucleotide donor. Also

α-L-fucosidases have been studied extensively since they catalyze the synthesis of 2'-FL through a transglycosylation reaction and often possess a higher availability and activity

in comparison with fucosyltransferases. The discovery of efficient transfucosidases and the availability of appropriate

fucosylated donor substrates will promote the application of α-L-fucosidases in the synthesis

of 2'-FL. In the near future

enzymatic synthesis is expected to become a method for industrial production of 2'-FL.

关键词

Keywords

references

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⁰

Enzymatic synthesis of 2'-fucosyllactose： advances and perspectives

DOI：10.12211/2096-8280.2020-033

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Abstract

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