植物合成生物学与母乳低聚糖生物制造

于文文; 吕雪芹; 李兆丰; 刘龙

doi:10.12211/2096-8280.2024-089

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植物合成生物学与母乳低聚糖生物制造

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- 植物合成生物学与母乳低聚糖生物制造
- Plant synthetic biology and bioproduction of human milk oligosaccharides
- 合成生物学 2025年6卷第5期页码：992-997
- 作者机构：
  
  1.江南大学未来食品科学中心，江苏无锡 214122
  2.江南大学生物工程学院，糖化学与生物技术教育部重点实验室，江苏无锡 214122
  3.江南大学食品学院，江苏无锡 214122
- 作者简介：
  
  [ "于文文（1994—），男，助理研究员。研究方向为乳基功能营养品的生物制造。E-mail：yuwenwen@stu.jiangnan.edu.cn" ]
  [ "刘龙（1980—），男，教授，博士生导师。主要从事合成生物学与食品生物制造的基础理论、关键技术与工程应用研究。E-mail：longliu@jiangnan.edu.cn" ]
- 基金信息：
  
  国家自然科学基金(32400055);中国博士后科学基金面上项目(2024M761180);博士后创新人才支持计划(BX20240145);江苏省卓越博士后计划(2024ZB009)
- DOI：10.12211/2096-8280.2024-089
  中图分类号： TS20;Q81
- 收稿：2024-12-02，
  
  修回：2025-01-10，
  
  纸质出版：2025-10-31
- 稿件说明：
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于文文，吕雪芹，李兆丰，刘龙. 植物合成生物学与母乳低聚糖生物制造［J］. 合成生物学， 2025， 6（5）： 992-997

YU Wenwen， LV Xueqin， LI Zhaofeng， LIU Long. Plant synthetic biology and bioproduction of human milk oligosaccharides［J］. Synthetic Biology Journal， 2025， 6（5）： 992-997
于文文，吕雪芹，李兆丰，刘龙. 植物合成生物学与母乳低聚糖生物制造［J］. 合成生物学， 2025， 6（5）： 992-997 DOI： 10.12211/2096-8280.2024-089.

YU Wenwen， LV Xueqin， LI Zhaofeng， LIU Long. Plant synthetic biology and bioproduction of human milk oligosaccharides［J］. Synthetic Biology Journal， 2025， 6（5）： 992-997 DOI： 10.12211/2096-8280.2024-089.

摘要

母乳低聚糖（human milk oligosaccharides，HMOs）是母乳中仅次于乳糖和脂肪的第三大固体成分，具有调节免疫系统、维持消化健康及促进大脑发育等生理功能。近期，加州大学伯克利分校Patrick M. Shih基于合成生物学使能技术，在模式植物本生烟中重构HMOs代谢合成途径，利用太阳光能将CO

转化为系列HMOs。相关研究为HMOs的生物制造提供了新思路与新范例，扩宽了植物合成生物学在乳基功能营养品可持续发展领域的应用前景。从政策批准和工业应用等角度来看，上述技术仍处于“概念验证”阶段；相比之下，将CO

捕获催化与微生物细胞工厂发酵过程相结合的技术方案，更有望在短期内实现HMOs绿色生物制造的工业应用。

Abstract

Human milk oligosaccharides (HMOs) are the third largest solid component in breast milk. They have a wide range of applications due to their beneficial physiological functions such as regulating the immune system

maintaining digestive health

and promoting brain development. There is a growing interest in the development of green and efficient bioproduction of HMOs

via

synthetic biology technologies. Recently

Patrick M. Shih’s team from the University of California

Berkeley

has engineered the model plant

Nicotiana benthamiana

as a photosynthetic platform for HMOs production. Specifically

the enzymes involved in HMOs biosynthesis were heterologously expressed in the cytosol to reconstruct the metabolic pathways required for HMOs bioproduction. Furthermore

they optimized the productions of HMOs by enhancing the supply of key precursors. Finally

several HMOs were successfully produced from the cost-effective raw materials CO

. The reported study provides deeper insights into the green bioproduction of HMOs

and expands the potential applications of plant synthetic biology technologies in the green and sustainable bioproduction of other dairy-based functional nutraceuticals. From the perspective of regulatory approval and industrial application

the aforementioned technology remains at the proof-of-concept stage. In contrast

an integrated approach combining CO

capture and conversion with microbial fermentation shows greater potential for demonstrating scalable green biomanufacturing of HMOs in the near term.

关键词

Keywords

references

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