乳酸菌的合成生物学工具及在合成益肤因子中的应用

郭婷婷; 韩湘凝; 黄熙婷; 张婷婷; 孔健

doi:10.12211/2096-8280.2024-071

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乳酸菌的合成生物学工具及在合成益肤因子中的应用

特约评述 | 更新时间：2025-05-20

- 乳酸菌的合成生物学工具及在合成益肤因子中的应用
- Advances in synthetic biology tools for lactic acid bacteria and their application in the development of skin beneficial products
- 合成生物学 2025年6卷第2期页码：320-333
- 作者机构：
  
  山东大学微生物改造技术全国重点实验室，山东青岛 266237
- 作者简介：
  
  [ "郭婷婷（1984—），女，副教授，硕士生导师。研究方向为乳酸菌遗传操作系统、乳酸菌合成生物学与活性产物的高效生产。E-mail：guotingting@sdu.edu.cn" ]
  [ "孔健（1964—），女，教授，博士生导师，研究方向为乳酸菌生理遗传及其应用，乳酸菌生物活性物质的开发等。E-mail：kongjian@sdu.edu.cn" ]
- 基金信息：
  
  微生物改造技术全国重点实验室“揭榜挂帅”项目(SKLMTFCP-2023-05)
- DOI：10.12211/2096-8280.2024-071
  中图分类号： Q939.9
- 收稿：2024-09-11，
  
  修回：2024-11-14，
  
  纸质出版：2025-04-30
- 稿件说明：
移动端阅览
郭婷婷，韩湘凝，黄熙婷，张婷婷，孔健. 乳酸菌的合成生物学工具及在合成益肤因子中的应用［J］. 合成生物学， 2025， 6（2）： 320-333

GUO Tingting， HAN Xiangning， HUANG Xiting， ZHANG Tingting， KONG Jian. Advances in synthetic biology tools for lactic acid bacteria and their application in the development of skin beneficial products［J］. Synthetic Biology Journal， 2025， 6（2）： 320-333
郭婷婷，韩湘凝，黄熙婷，张婷婷，孔健. 乳酸菌的合成生物学工具及在合成益肤因子中的应用［J］. 合成生物学， 2025， 6（2）： 320-333 DOI： 10.12211/2096-8280.2024-071.

GUO Tingting， HAN Xiangning， HUANG Xiting， ZHANG Tingting， KONG Jian. Advances in synthetic biology tools for lactic acid bacteria and their application in the development of skin beneficial products［J］. Synthetic Biology Journal， 2025， 6（2）： 320-333 DOI： 10.12211/2096-8280.2024-071.

摘要

乳酸菌在发酵食品中的使用有着悠久历史，一些菌种被认为是安全级微生物。乳酸菌也是人体正常的共生菌，具有调节肠道和皮肤微生态的菌群平衡、增强机体免疫力等有益作用。当前，乳酸菌及其产生的活性代谢产物添加到护肤品中产生保湿、抗氧化和减轻过敏等效果得到了消费者认可，而一些乳酸菌在缓解和治疗皮肤疾病方面的作用也有越来越多的数据支持。基于此，利用乳酸菌作为底盘生产护肤活性物质或作为皮肤修复的生物治疗载体具有广阔的应用前景。本文首先总结了在乳酸菌中建立的遗传操作系统，着重关注遗传可及性、基因表达系统和基因组编辑技术，然后总结了乳酸菌作为细胞工厂生产保湿因子和抗氧化产物的研究进展，最后介绍了工程乳酸菌针对皮肤损伤进行靶向递药的可行性，旨在为乳酸菌应用于皮肤健康领域提供借鉴。

Abstract

Lactic acid bacteria (LABs) are a group of Gram-positive bacteria that metabolize soluble carbohydrates to produce lactate as the main metabolite. Certain LABs have a long history of use in fermented foods and are generally considered as safe. On the other hand

LABs are commensal bacteria of the human body and have been shown to exert beneficial effect on the host

such as regulating the balance of intestinal and skin microecology and enhancing the body's immunity. At present

the addition of LABs and their active metabolites to skin care products could enhance moisturizing

antioxidant

and allergy-reduction effect

which have been accepted by consumers. The roles of LABs in treating skin diseases are also supported by more and more experimental data. With the rapid development of genetic tools

LABs have been explored to effectively produce value-added food and biomedical products such as organic acids

alcohols

and exopolysaccharides. Moreover

recent advances in synthetic biology have been used to engineer LABs for delivering therapeutic molecules in response to disease signals

showing attractive application prospect for disease therapy

situ

. In the field of skincare

LABs are an attractive chassis

for being engineered to produce bioactive substances for skincare and also as biotherapy carriers for wound treatment

as the food-safe status of LABs. In this review

we summarize the genetic operation systems established for LABs

from genetic accessibility to gene expression systems

and highlight the emerging genome editing techniques for manipulating their genomes. In addition

we comment research progress in producing moisturizing factors and antioxidants using LABs as cell factories. Finally

we expect the feasibility of targeted drug delivery by engineered LABs for healing skin wound and the prevention of infection by pathogenic bacteria

aiming at providing a reference for applying LABs in skin health.

关键词

Keywords

references

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