液体生物燃料合成与炼制的研究进展

郭姝媛; 张倩楠; 姑丽克孜· 买买提热夏提; 杨一群; 于涛

doi:10.12211/2096-8280.2023-040

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液体生物燃料合成与炼制的研究进展

特约评述 | 更新时间：2025-06-20

- 液体生物燃料合成与炼制的研究进展
- Advances in microbial production of liquid biofuels
- 合成生物学 2025年6卷第1期页码：18-44
- 作者机构：
  
  1.中国科学院深圳先进技术研究院，深圳合成生物学创新研究院，合成生物化学研究中心，广东深圳 518055
  2.中国科学院深圳先进技术研究院，深圳合成生物学创新研究院，中国科学院定量工程生物学重点实验室，广东深圳 518055
- 作者简介：
  
  [ "郭姝媛（1991—），女，博士，助理研究员。研究方向为甲醇生物转化及产物合成。 E-mail：sy.guo@siat.ac.cn" ]
  [ "于涛（1986—），男，博士，研究员。研究方向为酿酒酵母的合成生物学。 E-mail：tao.yu@siat.ac.cn" ]
- 基金信息：
  
  国家重点研发计划(2021YFA0911000;2020YFA0907800);广东省重点研发计划项目(2022B1111080005);国家自然科学基金(NSFC32071416);深圳合成生物学创新研究院科研基金(JCHZ20200003);深圳市科技计划(ZDSYS20210623091810032);中国科学院战略性先导科技专项(XDB0480000);招商局集团先进技术研究院有限公司（基于电催化CO2转化与生物炼制的绿色制造项目）;中海石油化学股份有限公司和海洋石油富岛有限公司“碳中和与粮食安全交叉创新联合实验室”项目;深圳先进院跨所联合攻关青年团队项目（电驱动CO2转化与生物炼制规模化示范）
- DOI：10.12211/2096-8280.2023-040
  中图分类号： Q815
- 收稿：2023-06-13，
  
  修回：2024-01-30，
  
  纸质出版：2025-01-31
- 稿件说明：
移动端阅览
郭姝媛，张倩楠，姑丽克孜·买买提热夏提，杨一群，于涛. 液体生物燃料合成与炼制的研究进展［J］. 合成生物学， 2025， 6（1）： 18-44

GUO Shuyuan， ZHANG Qiannan， Gulikezi·MAIMAITIREXIATI ， YANG Yiqun， YU Tao. Advances in microbial production of liquid biofuels［J］. Synthetic Biology Journal， 2025， 6（1）： 18-44
郭姝媛，张倩楠，姑丽克孜·买买提热夏提，杨一群，于涛. 液体生物燃料合成与炼制的研究进展［J］. 合成生物学， 2025， 6（1）： 18-44 DOI： 10.12211/2096-8280.2023-040.

GUO Shuyuan， ZHANG Qiannan， Gulikezi·MAIMAITIREXIATI ， YANG Yiqun， YU Tao. Advances in microbial production of liquid biofuels［J］. Synthetic Biology Journal， 2025， 6（1）： 18-44 DOI： 10.12211/2096-8280.2023-040.

摘要

人类社会发展对化石燃料的依赖导致了资源枯竭的加剧及显著的气候变化，迫切需要开发能够代替化石燃料的新型生物燃料。虽然已有生物乙醇和生物柴油等生物能源，但其生产规模和成本仍然是大规模应用的主要问题。近年来，随着可再生能源技术的发展，结合代谢工程及新兴的合成生物学，开发基于CO

合成的新兴生物燃料，逐渐成为未来绿色能源的重要研究方向。本文综述了生物燃料的种类及四代生物燃料的发展情况，并着重介绍了第三代和第四代生物燃料丰富的底物原材料、多能源偶联合成生物燃料的研究现状、合成生物学在其中的应用及现阶段的研究进展。最后概括了合成生物燃料所面临的困境，主要包括原料的供应及成本，新型液体生物燃料产量低和产品种类少等问题，并提出相应的解决办法，以二氧化碳作为主要原材料，结合自养型微生物及甲基营养型微生物等细胞工厂，通过优选固碳途径、转化二氧化碳为甲醇等低碳底物及多能源耦合等方式实现多种生物燃料的合成，以期扩大生物燃料的产能及应用范围，进一步推动新型生物燃料的产业化进程。

Abstract

With the socioeconomic development

the dependence of human beings on fossil fuels has led to their shortage and climate change. This has created an urgent need for alternatives that are renewable and environmentally friendly

and biofuels are one of them. Nowadays

widely recognized b

iofuels like fuel ethanol and biodiesel face challenges in terms of their production capacity due to limitation on raw materials such as grains and edible oils and high cost as well. Hence

the integration of metabolic engineering and synthetic biology has opened avenues for utilizing diverse substrates from other renewable sources

such as solar energy

light energy

electric energy

and waste biomass. Microbial cell factories

including microalgae

bacteria

and yeast

play a crucial role in synthesizing biofuels. The review comments on the evolution of the four generations of biofuels

encompassing fuel ethanol

biodiesel

bio-gasoline

jet and aviation fuels. We also discuss how microorganisms can be explored for producing the third- and fourth-generation biofuels from a variety of unconventional substrates such as carbon dioxide

methanol

and methane

multi-energy coupling to synthesize biofuels from lignocellulose by bacterial or yeast

conversion by microalgae or electrochemical-biological systems

the conversion of methanol and methane by methyltrophic microbes

and the application of synthetic biology. Furthermore

we overview biosynthetic pathways and engineering strategies for optimizing biofuels production. These strategies can convert raw materials to various fuel products

including fatty acids and esters

advanced alcohols and esters

isoprenoids

and polyketides. Finally

we highlight some challenges in biofuels production

including raw material supply and cost issue

low production yield

and limited product variety. Meanwhile

to address these challenges

we propose corresponding solutions. For example

by optimizing carbon fixation pathways

and converting carbon dioxide into low-carbon substrates like methanol

autotrophic microorganisms

methylotrophic microorganisms

and other cell factories can utilize carbon dioxide as the major raw material to synthesize various biofuels

which can benefit the application of biofuels and further promote their industrial production.

关键词

Keywords

references

IEA . World Energy Outlook 2022 [R/OL ] . [ 2023-04-01 ] . https://www.iea.org/reports/world-energy-outlook-2022 https://www.iea.org/reports/world-energy-outlook-2022 .

LIAO J C , MI L , PONTRELLI S , et al . Fuelling the future: microbial engineering for the production of sustainable biofuels [J ] . Nature Reviews Microbiology , 2016 , 14 ( 5 ): 288 - 304 .

AMJITH L , BAVANISH B . A review on biomass and wind as renewable energy for sustainable environment [J ] . Chemosphere , 2022 , 293 : 133579 .

INGANÄS O , SUNDSTRÖM V . Solar energy for electricity and fuels [J ] . Ambio , 2016 , 45 ( Suppl 1 ): S15 - S23 .

CHOWDHURY F A . Recent advances and demonstrated potentials for clean hydrogen via overall solar water splitting [J ] . MRS Advances , 2019 , 4 ( 51 ): 2771 - 2785 .

LYU S L , YOUNIS M A , LIU Z B , et al . Rational design on photoelectrodes and devices to boost photoelectrochemical performance of solar-driven water splitting: a mini review [J ] . Frontiers of Chemical Science and Engineering , 2022 , 16 ( 6 ): 777 - 798 .

DAS J , RAVISHANKAR H , LENS P N L . Biological biogas purification: recent developments, challenges and future prospects [J ] . Journal of Environmental Management , 2022 , 304 : 114198 .

DE TISSERA S , KÖPKE M , SIMPSON S D , et al . Syngas biorefinery and syngas utilization [J ] . Advances in Biochemical Engineering/Biotechnology , 2019 , 166 : 247 - 280 .

LIU Y Z , CRUZ-MORALES P , ZARGAR A , et al . Biofuels for a sustainable future [J ] . Cell , 2021 , 184 ( 6 ): 1636 - 1647 .

王冉 , 常家辉 , 白子龙 . 国外生物能源研究进展及应用概述 [J ] . 国防制造技术 , 2021 ( 4 ): 4 - 7 .

WANG R , CHANG J H , BAI Z L . Overview of research progress and application of bioenergy abroad [J ] . Defense Manufacturing Technology , 2021 ( 4 ): 4 - 7 .

CAVELIUS P , ENGELHART-STRAUB S , MEHLMER N , et al . The potential of biofuels from first to fourth generation [J ] . PLoS Biology , 2023 , 21 ( 3 ): e3002063 .

MUSSATTO S I , DRAGONE G , GUIMARÃES P M R , et al . Technological trends, global market, and challenges of bio-ethanol production [J ] . Biotechnology Advances , 2010 , 28 ( 6 ): 817 - 830 .

徐雪雯 , 李鹏辉 , 童国林 . 第二代生物乙醇制备研究进展 [J ] . 中国造纸 , 2023 , 42 ( 2 ): 94 - 101 .

XU X W , LI P H , TONG G L . Research progress of the preparation of second-generation bioethanol [J ] . China Pulp & Paper , 2023 , 42 ( 2 ): 94 - 101 .

BRODA M , YELLE D J , SERWAŃSKA K . Bioethanol production from lignocellulosic biomass—challenges and solutions [J ] . Molecules , 2022 , 27 ( 24 ): 8717 .

ISIKGOR F H , BECER C R . Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers [J ] . Polymer Chemistry , 2015 , 6 ( 25 ): 4497 - 4559 .

SCHWARZ W H . The cellulosome and cellulose degradation by anaerobic bacteria [J ] . Applied Microbiology and Biotechnology , 2001 , 56 ( 5-6 ): 634 - 649 .

方志锋 , 刘昆仑 , 陈复生 , 等 . 木质纤维素类生物质制备生物乙醇研究进展 [J ] . 现代化工 , 2013 , 33 ( 1 ): 30 - 33 .

FANG Z F , LIU K L , CHEN F S , et al . Research development of bioethanol preparation from lignocellulosic biomass [J ] . Modern Chemical Industry , 2013 , 33 ( 1 ): 30 - 33 .

SARAVANAN A , SENTHIL KUMAR P , JEEVANANTHAM S , et al . Recent advances and sustainable development of biofuels production from lignocellulosic biomass [J ] . Bioresource Technology , 2022 , 344 : 126203 .

OJEDA K , SÁNCHEZ E , EL-HALWAGI M , et al . Exergy analysis and process integration of bioethanol production from acid pre-treated biomass: comparison of SHF, SSF and SSCF pathways [J ] . Chemical Engineering Journal , 2011 , 176-177 : 195 - 201 .

MOHD AZHAR S H , ABDULLA R , JAMBO S A , et al . Yeasts in sustainable bioethanol production: a review [J ] . Biochemistry and Biophysics Reports , 2017 , 10 : 52 - 61 .

JOSHI S , MISHRA S . Recent advances in biofuel production through metabolic engineering [J ] . Bioresource Technology , 2022 , 352 : 127037 .

KAZEMI SHARIAT PANAHI H , DEHHAGHI M , DEHHAGHI S , et al . Engineered bacteria for valorizing lignocellulosic biomass into bioethanol [J ] . Bioresource Technology , 2022 , 344 : 126212 .

SIMAS-RODRIGUES C , VILLELA H D M , MARTINS A P , et al . Microalgae for economic applications: advantages and perspectives for bioethanol [J ] . Journal of Experimental Botany , 2015 , 66 ( 14 ): 4097 - 4108 .

MEDIPALLY S R , YUSOFF F M , BANERJEE S , et al . Microalgae as sustainable renewable energy feedstock for biofuel production [J ] . BioMed Research International , 2015 , 2015 : 519513 .

刘雪艳 , 苏忠亮 . 微藻生物燃料的研究进展 [J ] . 化学与生物工程 , 2017 , 34 ( 3 ): 11 - 14 .

LIU X Y , SU Z L . Research progress on microalgae biofuel [J ] . Chemistry & Bioengineering , 2017 , 34 ( 3 ): 11 - 14 .

GILMOUR D J . Microalgae for biofuel production [J ] . Advances in Applied Microbiology , 2019 , 109 : 1 - 30 .

MAIA J L D , CARDOSO J S , MASTRANTONIO D J D S , et al . Microalgae starch: a promising raw material for the bioethanol production [J ] . International Journal of Biological Macromolecules , 2020 , 165 ( Pt B ): 2739 - 2749 .

LAKATOS G E , RANGLOVÁ K , MANOEL J C , et al . Bioethanol production from microalgae polysaccharides [J ] . Folia Microbiologica , 2019 , 64 ( 5 ): 627 - 644 .

GRAMA S B , LIU Z Y , LI J . Emerging trends in genetic engineering of microalgae for commercial applications [J ] . Marine Drugs , 2022 , 20 ( 5 ): 285 .

JAISWAL A , BABU V , BAISHYA B , et al . Biochemical pathways regulated by algae to mitigate global carbon emissions: a review [J ] . Journal of Environmental Pathology, Toxicology and Oncology , 2020 , 39 ( 4 ): 317 - 334 .

LIU H , SUN J L , CHANG J S , et al . Engineering microbes for direct fermentation of cellulose to bioethanol [J ] . Critical Reviews in Biotechnology , 2018 , 38 ( 7 ): 1089 - 1105 .

SINGH A , SINGHANIA R R , SOAM S , et al . Production of bioethanol from food waste: status and perspectives [J ] . Bioresource Technology , 2022 , 360 : 127651 .

SHARMA J , KUMAR V , PRASAD R , et al . Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: recent advancements and current challenges [J ] . Biotechnology Advances , 2022 , 56 : 107925 .

KUMAR K , DASGUPTA C N , NAYAK B , et al . Development of suitable photobioreactors for CO 2 sequestration addressing global warming using green algae and cyanobacteria [J ] . Bioresource Technology , 2011 , 102 ( 8 ): 4945 - 4953 .

林海龙 , 林鑫 , 岳国君 . 我国生物燃料乙醇产业新进展 [J ] . 新能源进展 , 2020 , 8 ( 3 ): 165 - 171 .

LIN H L , LIN X , YUE G J . New advances in China’s biofuel ethanol industry [J ] . Advances in New and Renewable Energy , 2020 , 8 ( 3 ): 165 - 171 .

KAMARAJ R , RAO Y K S S , BALAKRISHNA B . Biodiesel blends: a comprehensive systematic review on various constraints [J ] . Environmental Science and Pollution Research International , 2022 , 29 ( 29 ): 43770 - 43785 .

MATHEW G M , RAINA D , NARISETTY V , et al . Recent advances in biodiesel production: challenges and solutions [J ] . Science of the Total Environment , 2021 , 794 : 148751 .

王欣竹 . 从工程微藻中提取制备生物柴油的研究进展 [J ] . 当代化工研究 , 2022 ( 2 ): 162 - 164 .

WANG X Z . Research progress of biodiesel extraction from engineering microalgae [J ] . Modern Chemical Research , 2022 ( 2 ): 162 - 164 .

SINGH A , NIGAM P S , MURPHY J D . Mechanism and challenges in commercialisation of algal biofuels [J ] . Bioresource Technology , 2011 , 102 ( 1 ): 26 - 34 .

BRAR A , KUMAR M , SONI T , et al . Insights into the genetic and metabolic engineering approaches to enhance the competence of microalgae as biofuel resource: a review [J ] . Bioresource Technology , 2021 , 339 : 125597 .

TAPARIA T , MVSS M , MEHROTRA R , et al . Developments and challenges in biodiesel production from microalgae: a review [J ] . Biotechnology and Applied Biochemistry , 2016 , 63 ( 5 ): 715 - 726 .

GONG Y , MIAO X L . Short chain fatty acid biosynthesis in microalgae Synechococcus sp. PCC 7942 [J ] . Marine Drugs , 2019 , 17 ( 5 ): 255 .

SUN X M , REN L J , ZHAO Q Y , et al . Enhancement of lipid accumulation in microalgae by metabolic engineering [J ] . Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids , 2019 , 1864 ( 4 ): 552 - 566 .

董文婉 . 航空生物燃料作为新型能源的发展与利弊 [J ] . 民航管理 , 2013 ( 11 ): 111 - 112 .

DONG W W . The development of aviation biofuel and the pros and cons [J ] . Civil Aviation Management , 2013 ( 11 ): 111 - 112 .

MASCAL M , DUTTA S . Synthesis of highly-branched alkanes for renewable gasoline [J ] . Fuel Processing Technology , 2020 , 197 : 106192 .

EL-DALATONY M M , SAHA S , GOVINDWAR S P , et al . Biological conversion of amino acids to higher alcohols [J ] . Trends in Biotechnology , 2019 , 37 ( 8 ): 855 - 869 .

MEHRER C R , INCHA M R , POLITZ M C , et al . Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway [J ] . Metabolic Engineering , 2018 , 48 : 63 - 71 .

DELLOMONACO C , CLOMBURG J M , MILLER E N , et al . Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals [J ] . Nature , 2011 , 476 ( 7360 ): 355 - 359 .

GUO L , DIAO W W , GAO C , et al . Engineering Escherichia coli lifespan for enhancing chemical production [J ] . Nature Catalysis , 2020 , 3 : 307 - 318 .

SHEN C R , LAN E I , DEKISHIMA Y , et al . Driving for ces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli [J ] . Applied and Environmental Microbiology , 2011 , 77 ( 9 ): 2905 - 2915 .

ATSUMI S , HANAI T , LIAO J C . Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels [J ] . Nature , 2008 , 451 ( 7174 ): 86 - 89 .

PARK S H , HAHN J S . Development of an efficient cytosolic isobutanol production pathway in Saccharomyces cerevisiae by optimizing copy numbers and expression of the pathway genes based on the toxic effect of α-acetolactate [J ] . Scientific Reports , 2019 , 9 ( 1 ): 3996 .

AVALOS J L , FINK G R , STEPHANOPOULOS G . Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols [J ] . Nature Biotechnology , 2013 , 31 ( 4 ): 335 - 341 .

WESS J , BRINEK M , BOLES E . Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation [J ] . Biotechnology for Biofuels , 2019 , 12 : 173 .

VOGT M , BRÜSSELER C , VAN OOYEN J , et al . Production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered Corynebacterium glutamicum [J ] . Metabolic Engineering , 2016 , 38 : 436 - 445 .

SIRIPONG W , ANGELA C , TANAPONGPIPAT S , et al . Metabolic engineering of Pichia pastoris for production of isopentanol (3-methyl-1-butanol) [J ] . Enzyme and Microbial Technology , 2020 , 138 : 109557 .

SIRIPONG W , WOLF P , KUSUMOPUTRI T P , et al . Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate [J ] . Biotechnology for Biofuels , 2018 , 11 : 1 .

SU H F , LIN J F , WANG Y H , et al . Engineering Brevibacterium flavum for the production of renewable bioenergy: C 4 –C 5 advanced alcohols [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 9 ): 1946 - 1958 .

LI S S , WEN J P , JIA X Q . Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression [J ] . Applied Microbiology and Biotechnology , 2011 , 91 ( 3 ): 577 - 589 .

SHI X Y , PARK H M , KIM M H , et al . Isopropanol biosynthesis from crude glycerol using fatty acid precursors via engineered oleaginous yeast Yarrowia lipolytica [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 168 .

YUZAWA S , MIRSIAGHI M , JOCIC R , et al . Short-chain ketone production by engineered polyketide syn thases in Streptomyces albus [J ] . Nature Communications , 2018 , 9 ( 1 ): 4569 .

GROUSSEAU E , LU J N , GORRET N , et al . Isopropanol production with engineered Cupriavidus necator as bioproduction platform [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 9 ): 4277 - 4290 .

ZHANG Y , SONG X H , LAI Y M , et al . High-yielding terpene-based biofuel production in Rhodobacter capsulatus [J ] . ACS Synthetic Biology , 2021 , 10 ( 6 ): 1545 - 1552 .

PERALTA-YAHYA P P , OUELLET M , CHAN R , et al . Identification and microbial production of a terpene-based advanced biofuel [J ] . Nature Communications , 2011 , 2 : 483 .

LIU C L , BI H R , BAI Z H , et al . Engineering and manipulation of a mevalonate pathway in Escherichia coli for isoprene production [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 1 ): 239 - 250 .

ALONSO-GUTIERREZ J , CHAN R , BATTH T S , et al . Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production [J ] . Metabolic Engineering , 2013 , 19 : 33 - 41 .

MEADOWS A L , HAWKINS K M , TSEGAYE Y , et al . Rewriting yeast central carbon metabolism for industrial isoprenoid production [J ] . Nature , 2016 , 537 : 694 - 697 .

SHI T Q , LI Y W , ZHU L , et al . Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction [J ] . Biotechnology Journal , 2021 , 16 ( 7 ): e2100097 .

LIU Y H , JIANG X , CUI Z Y , et al . Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene [J ] . Biotechnology for Biofuels , 2019 , 12 : 296 .

FANG L X , FAN J , LUO S L , et al . Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids [J ] . Nature Communications , 2021 , 12 ( 1 ): 4976 .

PAN H , ZHANG L H , LI X , et al . Biosynthesis of the fatty acid isopropyl esters by engineered Escherichia coli [J ] . Enzyme and Microbial Technology , 2017 , 102 : 49 - 52 .

GUO D Y , ZHU J , DENG Z X , et al . Metabolic engineering of Escherichia coli for production of fatty acid short-chain esters through combination of the fatty acid and 2-keto acid pathways [J ] . Metabolic Engineering , 2014 , 22 : 69 - 75 .

YANG L , ZHU Z , WANG W H , et al . Microbial recycling of glycerol to biodiesel [J ] . Bioresource Technology , 2013 , 150 : 1 - 8 .

D’ESPAUX L , GHOSH A , RUNGUPHAN W , et al . Engineering high-level production of fatty alcohols by Saccharomyces cerevisiae from lignocellulosic feedstocks [J ] . Metabolic Engineering , 2017 , 42 : 115 - 125 .

YU T , ZHOU Y J , WENNING L , et al . Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals [J ] . Nature Communications , 2017 , 8 : 15587 .

YU T , ZHOU Y J , HUANG M T , et al . Reprogramming yeast metabolism from alcoholic fermentation to lipogenesis [J ] . Cell , 2018 , 174 ( 6 ): 1549 - 1558.e14 .

ZHU Z W , HU Y T , TEIXEIRA P G , et al . Multidimensional engineering of Saccharomyces cerevisiae for efficient synthesis of medium-chain fatty acids [J ] . Nature Catalysis , 2020 , 3 : 64 - 74 .

YU T , LIU Q L , WANG X , et al . Metabolic reconfiguration enables synthetic reductive metabolism in yeast [J ] . Nature Metabolism , 2022 , 4 ( 11 ): 1551 - 1559 .

WANG W , WEI H , KNOSHAUG E , et al . Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica [J ] . Biotechnology for Biofuels , 2016 , 9 : 227 .

JANG Y S , LEE J Y , LEE J M , et al . Enhanced butanol production obtained by reinforcing the direct butanol-forming route in Clostridium acetobutylicum [J ] . mBio , 2012 , 3 ( 5 ): e00314-12 .

LIU H H , SONG Y L , FAN X , et al . Yarrowia lipolytica as an oleaginous platform for the production of value-added fatty acid-based bioproducts [J ] . Frontiers in Microbiology , 2021 , 11 : 608662 .

ZHOU Y J , BUIJS N A , ZHU Z W , et al . Harnessing yeast peroxisomes for biosynthesis of fatty-acid-derived biofuels and chemicals with relieved side-pathway competition [J ] . Journal of the American Chemical Society , 2016 , 138 ( 47 ): 15368 - 15377 .

ZHAO Y K , ZHU K , LI J , et al . High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica [J ] . Microbial Biotechnology , 2021 , 14 ( 6 ): 2497 - 2513 .

LI H , OPGENORTH P H , WERNICK D G , et al . Integrated electromicrobial conversion of CO 2 to higher alcohols [J ] . Science , 2012 , 335 ( 6076 ): 1596 .

LIU C , COLÓN B C , ZIESACK M , et al . Water splitting-biosynthetic system with CO₂ reduction efficiencies exceeding photosynthesis [J ] . Science , 2016 , 352 ( 6290 ): 1210 - 1213 .

HENSTRA A M , SIPMA J , RINZEMA A , et al . Microbiology of synthesis gas fermentation for biofuel production [J ] . Current Opinion in Biotechnology , 2007 , 18 ( 3 ): 200 - 206 .

WILLCOX D , CHAPPELL B G N , HOGG K F , et al . A general catalytic β-C-H carbonylation of aliphatic amines to β-lactams [J ] . Science , 2016 , 354 ( 6314 ): 851 - 857 .

KIRST H , GABILLY S T , NIYOGI K K , et al . Photosynthetic antenna engineering to improve crop yields [J ] . Planta , 2017 , 245 ( 5 ): 1009 - 1020 .

SOUTH P F , CAVANAGH A P , LIU H W , et al . Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field [J ] . Science , 2019 , 363 ( 6422 ): eaat9077 .

WU I , ARNOLD F H . Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures [J ] . Biotechnology and Bioengineering , 2013 , 110 ( 7 ): 1874 - 1883 .

LI P H , REN J P , JIANG Z W , et al . Review on the preparation of fuels and chemicals based on lignin [J ] . RSC Advances , 2022 , 12 ( 17 ): 10289 - 10305 .

ZHANG Y H P , LYND L R . Cellulose utilization by Clostridium thermocellum : bioenergetics and hydrolysis product assimilation [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2005 , 102 ( 20 ): 7321 - 7325 .

LEE J W , YOOK S D , KOH H G , et al . Engineering xylose metabolism in yeasts to produce biofuels and chemicals [J ] . Current Opinion in Biotechnology , 2021 , 67 : 15 - 25 .

RUCHALA J , SIBIRNY A A . Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts [J ] . FEMS Microbiology Reviews , 2021 , 45 ( 4 ): fuaa069 .

GONG C J , CAO L P , FANG D L , et al . Genetic manipulation strategies for ethanol production from bioconversion of lignocellulose waste [J ] . Bioresource Technology , 2022 , 352 : 127105 .

KRICKA W , FITZPATRICK J , BOND U . Challenges for the production of bioethanol from biomass using recombinant yeasts [J ] . Advances in Applied Microbiology , 2015 , 92 : 89 - 125 .

KATAHIRA S , MIZUIKE A , FUKUDA H , et al . Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain [J ] . Applied Microbiology and Biotechnology , 2006 , 72 ( 6 ): 1136 - 1143 .

STEEN E J , KANG Y S , BOKINSKY G , et al . Microbial production of fatty-acid-derived fuels and chemicals from plant biomass [J ] . Nature , 2010 , 463 ( 7280 ): 559 - 562 .

XUE D S , YAO D H , SUKUMARAN R K , et al . Tandem integration of aerobic fungal cellulase production, lignocellulose substrate saccharification and anaerobic ethanol fermentation by a modified gas lift bioreactor [J ] . Bioresource Technology , 2020 , 302 : 122902 .

JIANG Y J , DONG W L , XIN F X , et al . Designing synthetic microbial consortia for biofuel production [J ] . Trends in Biotechnology , 2020 , 38 ( 8 ): 828 - 831 .

BROWN J L , PERISIN M A , SWIFT C L , et al . Co-cultivation of anaerobic fungi with Clostridium acetobutylicum bolsters butyrate and butanol production from cellulose and lignocellulose [J ] . Journal of Industrial Microbiology & Biotechnology , 2023 , 49 ( 6 ): kuac024 .

YOU S P , CHANG H X , ZHANG C Y , et al . Recycling strategy and repression elimination for lignocellulosic-based farnesene production with an engineered Escherichia coli [J ] . Journal of Agricultural and Food Chemistry , 2019 , 67 ( 35 ): 9858 - 9867 .

WANG S M , WANG Z B , WANG Y C , et al . Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method [J ] . Biotechnology for Biofuels , 2017 , 10 : 297 .

CHEN C T , CHEN F Y H , BOGORAD I W , et al . Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent grow th and production [J ] . Metabolic Engineering , 2018 , 49 : 257 - 266 .

WANG C , REN J , ZHOU L B , et al . An aldolase-catalyzed new metabolic pathway for the assimilation of formaldehyde and methanol to synthesize 2-keto-4-hydroxybutyrate and 1,3-propanediol in Escherichia coli [J ] . ACS Synthetic Biology , 2019 , 8 ( 11 ): 2483 - 2493 .

BENNETT R K , DILLON M , GERALD HAR J R , et al . Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production [J ] . Metabolic Engineering , 2020 , 60 : 45 - 55 .

BENNETT R K , GONZALEZ J E , WHITAKER W B , et al . Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph [J ] . Metabolic Engineering , 2018 , 45 : 75 - 85 .

MA Z X , ZHANG M , ZHANG C T , et al . Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1 [J ] . Biotechnology Journal , 2021 , 16 ( 6 ): e2000413 .

HU B , YANG Y M , BECK D A , et al . Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance [J ] . Biotechnology for Biofuels , 2016 , 9 : 84 .

YUAN X J , CHEN W J , MA Z X , et al . Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens [J ] . Metabolic Engineering , 2021 , 64 : 95 - 110 .

DAVIES F K , WORK V H , BELIAEV A S , et al . Engineering limonene and bisabolene production in wild type and a glycogen-deficient mutant of Synechococcus sp. PCC 7002 [J ] . Frontiers in Bioengineering and Biotechnology , 2014 , 2 : 21 .

GARRIGUES L , MAIGNIEN L , LOMBARD E , et al . Isopropanol production from carbon dioxide in Cupriavidus necator in a pressur ized bioreactor [J ] . New Biotechnology , 2020 , 56 : 16 - 20 .

LAUER I , PHILIPPS G , JENNEWEIN S . Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO 2 and H 2 [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 85 .

ZHENG T T , ZHANG M L , WU L H , et al . Upcycling CO 2 into energy-rich long-chain compounds via electrochemical and metabolic engineering [J ] . Nature Catalysis , 2022 , 5 : 388 - 396 .

CAI P , WU X Y , DENG J , et al . Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2022 , 119 ( 29 ): e2201711119 .

LI Z K , XIONG B , LIU L , et al . Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory [J ] . Journal of Industrial Microbiology & Biotechnology , 2019 , 46 ( 6 ): 783 - 790 .

GAO J Q , LI Y X , YU W , et al . Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol [J ] . Nature Metabolism , 2022 , 4 ( 7 ): 932 - 943 .

YUNUS I S , PALMA A , TRUDEAU D L , et al . Methanol-free biosynthesis of fatty acid methyl ester (FAME) in Synechocystis sp. PCC 6803 [J ] . Metabolic Engineering , 2020 , 57 : 217 - 227 .

YOU S K , PARK H M , LEE M E , et al . Non-photosynthetic CO 2 utilization to increase fatty acid production in Yarrowia lipolytica [J ] . Journal of Agricultural and Food Chemistry , 2021 , 69 ( 40 ): 11912 - 11918 .

ZHU W L , CUI J Y , CUI L Y , et al . Bioconversion of methanol to value-added mevalonate by engineered Methylobacterium extorquens AM1 containing an optimized mevalonate pathway [J ] . Applied Microbiology and Biotechnology , 2016 , 100 ( 5 ): 2171 - 2182 .

GRENZ S , BAUMANN P T , RÜCKERT C , et al . Exploiting Hydrogenophaga pseudoflava for aerobic syngas-based production of chemicals [J ] . Metabolic Engineering , 2019 , 55 : 220 - 230 .

KRIEG T , SYDOW A , FAUST S , et al . CO 2 to terpenes: autotrophic and electroautotrophic α-humulene production with Cupriavidus necator [J ] . Angewandte Chemie International Edition , 2018 , 57 ( 7 ): 1879 - 1882 .

NGUYEN A D , KIM D H , LEE E Y . Unlocking the biosynthesis of sesquiterpenoids from methane via the methylerythritol phosphate pathway in methanotrophic bacteria, using α-humulene as a model compound [J ] . Metabolic Engineering , 2020 , 61 : 69 - 78 .

YUNUS I S , ANFELT J , SPORRE E , et al . Synthetic metabolic pathways for conversion of CO 2 into secreted short-to medium-chain hydrocarbons using cyanobacteria [J ] . Metabolic Engineering , 2022 , 72 : 14 - 23 .

LI M , LONG B , DAI S Y , et al . Altered carbon partitioning enhances CO 2 to terpene conversion in cyanobacteria [J ] . Biodesign Research , 2022 , 2022 : 9897425 .

ANTHONY J , RANGAMARAN V R , GOPAL D , et al . Ultraviolet and 5 ' fluorodeoxyuridine induced random mutagenesis in Chlorella vulgaris and its impact on fatty acid profile: a new insight on lipid-metabolizing genes and structural characterization of related proteins [J ] . Marine Biotechnology , 2015 , 17 ( 1 ): 66 - 80 .

BOYLE N R , PAGE M D , LIU B S , et al . Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas [J ] . The Journal of Biological Chemistry , 2012 , 287 ( 19 ): 15811 - 15825 .

BECKMANN J , LEHR F , FINAZZI G , et al . Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii [J ] . Journal of Biotechnology , 2009 , 142 ( 1 ): 70 - 77 .

MELIS A . Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency [J ] . Plant Science , 2009 , 177 ( 4 ): 272 - 280 .

MUSSGNUG J H , THOMAS-HALL S , RUPPRECHT J , et al . Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion [J ] . Plant Biotechnology Journal , 2007 , 5 ( 6 ): 802 - 814 .

LIANG F Y , ENGLUND E , LINDBERG P , et al . Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio [J ] . Metabolic Engineering , 2018 , 46 : 51 - 59 .

LI-BEISSON Y , THELEN J J , FEDOSEJEVS E , et al . The lipid biochemistry of eukaryotic algae [J ] . Progress in Lipid Research , 2019 , 74 : 31 - 68 .

PANICH J , FONG B , SINGER S W . Metabolic engineering of Cupriavidus necator H16 for sustainable biofuels from CO 2 [J ] . Trends in Biotechnology , 2021 , 39 ( 4 ): 412 - 424 .

KROG A , HEGGESET T M B , MÜLLER J E N , et al . Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasm id born NAD + dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties [J ] . PLoS One , 2013 , 8 ( 3 ): e59188 .

KRACKE F , LAI B , YU S Q , et al . Balancing cellular redox metabolism in microbial electrosynthesis and electro fermentation — a chance for metabolic engineering [J ] . Metabolic Engineering , 2018 , 45 : 109 - 120 .

NEVIN K P , HENSLEY S A , FRANKS A E , et al . Electrosynthesis of organic compounds from carbon dioxide is catalyzed by a diversity of acetogenic microorganisms [J ] . Applied and Environmental Microbiology , 2011 , 77 ( 9 ): 2882 - 2886 .

郭姝媛 , 吴良焕 , 刘香健 , 等 . 微生物中一碳代谢网络构建的进展与挑战 [J ] . 合成生物学 , 2022 , 3 ( 1 ): 116 - 137 .

GUO S Y , WU L H , LIU X J , et al . Developing C 1 -based metabolic network in methylotrophy for biotransformation [J ] . Synthetic Biology Journal , 2022 , 3 ( 1 ): 116 - 137 .

YU H , LIAO J C . A modified serine cycle in Escherichia coli coverts methanol and CO 2 to two-carbon compounds [J ] . Nature Communications , 2018 , 9 ( 1 ): 3992 .

BOGORAD I W , CHEN C T , THEISEN M K , et al . Building carbon-carbon bonds using a biocatalytic methanol condensation cycle [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2014 , 111 ( 45 ): 15928 - 15933 .

BAR-EVEN A . Formate assimilation: the metabolic architecture of natural and synthetic pathways [J ] . Biochemistry , 2016 , 55 ( 28 ): 3851 - 3863 .

YISHAI O , LINDNER S N , GONZALEZ DE LA CRUZ J , et al . The formate bio-economy [J ] . Current Opinion in Chemical Biology , 2016 , 35 : 1 - 9 .

HU G P , LI Z H , MA D L , et al . Light-driven CO 2 sequestration in Escherichia coli to achieve theoretical yield of chemicals [J ] . Nature Catalysis , 2021 , 4 : 395 - 406 .

LING H , PRATOMO JUWONO N K , TEO W S , et al . Engineering transcription factors to improve tolerance against alkane biofuels in Saccharomyces cerevisiae [J ] . Biotechnology for Biofuels , 2015 , 8 : 231 .

YAO P , YOU S P , QI W , et al . Investigation of fermentation conditions of biodiesel by-products for high production of β-farnesene by an engineered Escherichia coli [J ] . Environmental Science and Pollution Research International , 2020 , 27 ( 18 ): 22758 - 22769 .

ZHOU Y K , LI G , DONG J K , et al . MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae [J ] . Metabolic Engineering , 2018 , 47 : 294 - 302 .

LIU Z H , WANG K , CHEN Y , et al . Third-generation biorefineries as the means to produce fuels and chemicals from CO 2 [J ] . Nature Catalysis , 2020 , 3 : 274 - 288 .

TAN X Y , NIELSEN J . The integration of bio-catalysis and electrocatalysis to produce fuels and chemicals from carbon dioxide [J ] . Chemical Society Reviews , 2022 , 51 ( 11 ): 4763 - 4785 .

HU Q S , HU H T , CUI L , et al . Ultrafast electron transfer in Au-cyanobacteria hybrid for solar to chemical production [J ] . ACS Energy Letters , 2023 , 8 ( 1 ): 677 - 684 .

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