Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds

ZHAO Liang; LI Zhenshuai; FU Liping; LYU Ming; WANG Shi’an; ZHANG Quan; LIU Licheng; LI Fuli; LIU Ziyong

doi:10.12211/2096-8280.2024-013

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Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds

Invited Review | 更新时间：2025-04-11

- Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds
- Synthetic Biology Journal Vol. 5, Issue 6, Pages: 1300-1318(2024)
- 作者机构：
  
  1.中国科学院青岛生物能源与过程研究所，青岛市碳一炼制工程研究中心，中国科学院生物燃料重点实验室，山东青岛 266101
  2.山东能源研究院，山东青岛 266101
  3.中国海洋大学，山东青岛 266100
  4.青岛新能源山东省实验室，山东青岛 266101
  5.中国石油化工股份有限公司大连研究院，辽宁大连 116045
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2024-013
  CLC： Q816
- Received：04 February 2024，
  
  Revised：2024-05-08，
  
  Published：31 December 2024
- 稿件说明：
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赵亮，李振帅，付丽平，吕明，王士安，张全，刘立成，李福利，刘自勇. 生物转化一碳化合物原料产油脂与单细胞蛋白研究进展［J］. 合成生物学， 2024， 5（6）：1300-1318

ZHAO Liang， LI Zhenshuai， FU Liping， LYU Ming， WANG Shi’an， ZHANG Quan， LIU Licheng， LI Fuli， LIU Ziyong. Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds［J］. Synthetic Biology Journal， 2024， 5（6）： 1300-1318
赵亮，李振帅，付丽平，吕明，王士安，张全，刘立成，李福利，刘自勇. 生物转化一碳化合物原料产油脂与单细胞蛋白研究进展［J］. 合成生物学， 2024， 5（6）：1300-1318 DOI： 10.12211/2096-8280.2024-013.

ZHAO Liang， LI Zhenshuai， FU Liping， LYU Ming， WANG Shi’an， ZHANG Quan， LIU Licheng， LI Fuli， LIU Ziyong. Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds［J］. Synthetic Biology Journal， 2024， 5（6）： 1300-1318 DOI： 10.12211/2096-8280.2024-013.

摘要

一碳化合物是一类产生于自然界或工业过程中的液态或气态物质，其具有来源广泛、价格低廉、可持续生产的优势，有望成为新一代生物制造关键原料，包括液态的甲醇、甲酸，以及气态的CO

、CO、CH

等。在生物制造蓬勃发展的背景下，通过合成生物学手段改造微生物，使之利用一碳化合物高效生产油脂与单细胞蛋白等高附加值产品，降低对粮食、化石资源进口的依赖，成为缓解粮食能源危机的有效战略举措。本文综述了甲基营养型微生物、产乙酸菌以及酵母等微生物通过代谢途径、底盘遗传改造等方法，将一碳化合物转化为高附加值油脂与单细胞蛋白的最新研究进展；介绍了一些通过发酵工艺控制优化分子工程菌株利用一碳化合物的相关研究；同时收集了部分一碳化合物转化相关研究机构或企业的产业化案例。最后，针对一碳化合物利用菌株的代谢通路设计与遗传工具存在的限制问题，以及产乙酸菌与产油微生物之间的能量转化矛盾，展望了未来生物制造油脂与单细胞蛋白的前景和面临的挑战，提出在复杂系统性的生物制造过程中，发展多学科交叉的高效系统集成发酵，以期对一碳化合物的生物转化研究产生推动作用，为攻克目前存在的理论与实践难题提供新思路，并对实际应用与

产业化发展提供参考。

Abstract

One-carbon compounds are liquid or gaseous substances that can be naturally occurring or produced in industrial processes

offering the advantages of being abundant

cost-effective

and sustainable to produce. They are anticipated to serve as fundamental raw materials for the next phase of bio-manufacturing

encompassing easily transportable and storable liquid methanol

formic acid

and gaseous CO

and CH

. China is currently focusing on reducing carbon emissions and aims to progressively achieve the targets of carbon peak and carbon neutrality through diverse approaches. Amidst the flourishing landscape of bio-manufacturing

microorganisms are being genetically manipulated using synthetic biology techniques to efficiently harness one-carbon compounds for the creation of high-value products like lipids and single-cell protein. This initiative aims to reduce dependence on imported food and fossil resources

serving as a strategic measure to alleviate food and energy crises. This review presents a comprehensive overview of the most recent advancements in converting one-carbon compounds into valuable oils and single-cell proteins through the utilization of metabolic pathways

chassis genetic modification

and other methodologies involving methylotrophic microorganisms

acetogenic bacteria

yeast

and other microorganisms. It discusses pertinent studies on enhancing molecularly engineered strains through the fermentation process using one-carbon compounds and includes research cases focusing on the production of ultra-long-chain fatty acids. Furthermore

it collates industrial instances related to the conversion of one-carbon compounds from research institutions or companies. Lastly

by addressing the constraints in metabolic pathway design and genetic tools for utilizing one-carbon compound strains

as well as the energy conversion challenges between acetogenic bacteria and lipids-producing microorganisms

it offers foresight into the future opportunities and obstacles encountered in the bio-manufacturing of lipids and single-cell proteins. It suggests advancing inter-disciplinary

efficient systematic integration for fermentation within complex systemic bio-manufacturing processes

driving exploration on the biological conversion of one-carbon compounds

proposing novel solutions to current theoretical and practical challenges

and providing guidance for practical applications and industrial advancements.

关键词

Keywords

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