Design, optimization and application of synthetic carbon-negative phototrophic community

ZHENG Haotian; LI Chaofeng; LIU Liangxu; WANG Jiawei; LI Hengrun; NI Jun

doi:10.12211/2096-8280.2024-001

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Design, optimization and application of synthetic carbon-negative phototrophic community

Invited Review | 更新时间：2025-03-19

- Design, optimization and application of synthetic carbon-negative phototrophic community
- Synthetic Biology Journal Vol. 5, Issue 5, Pages: 1189-1210(2024)
- 作者机构：
  
  1.上海交通大学生命科学技术学院，微生物代谢国家重点实验室，上海 200240
  2.上海交通大学张江高等研究院，上海 201203
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2024-001
  CLC： Q819
- Received：02 January 2024，
  
  Revised：2024-04-16，
  
  Published：31 October 2024
- 稿件说明：
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郑皓天，李朝风，刘良叙，王嘉伟，李恒润，倪俊. 负碳人工光合群落的设计、优化与应用［J］. 合成生物学， 2024， 5（5）： 1189-1210

ZHENG Haotian， LI Chaofeng， LIU Liangxu， WANG Jiawei， LI Hengrun， NI Jun. Design， optimization and application of synthetic carbon-negative phototrophic community［J］. Synthetic Biology Journal， 2024， 5（5）： 1189-1210
郑皓天，李朝风，刘良叙，王嘉伟，李恒润，倪俊. 负碳人工光合群落的设计、优化与应用［J］. 合成生物学， 2024， 5（5）： 1189-1210 DOI： 10.12211/2096-8280.2024-001.

ZHENG Haotian， LI Chaofeng， LIU Liangxu， WANG Jiawei， LI Hengrun， NI Jun. Design， optimization and application of synthetic carbon-negative phototrophic community［J］. Synthetic Biology Journal， 2024， 5（5）： 1189-1210 DOI： 10.12211/2096-8280.2024-001.

摘要

生物转化技术的开发为解决能源转型和气候变化问题提供了可能。人工光合群落是由密切协作的光合自养微生物与异养微生物组成的新一代生物炼制平台，能够通过群落成员间的互利代谢分工，高效地利用光能将CO

直接转化为生物质和多种化学品，是实现负碳生物制造的潜在途径之一，因其在适用性和鲁棒性方面的优势受到了广泛的关注。近年来，随着系统生物学研究和合成生物技术的快速发展，多种研究策略被用于人工光合群落的设计与优化，取得了长远的进展，促进了对光合群落生产的理解。本文综述了光合群落的互作机制、独到优势和系统生物学研究方法，着重介绍人工光合群落的底盘升级、固定化/区室化技术、加强内部多层次调控等设计与优化策略以及在不同领域的应用进展。在此基础上，探讨了进一步将人工光合群落规模化应用所面临的光合效率和中间碳水化合物限制以及外源生物污染等潜在挑战，对纳米半导体材料杂合、物种间精细互作关系调控、基于多组学数据的群落模型构建等未来的改造方向和研究策略进行了展望。

Abstract

The extensive consumption of fossil oil and the rapid accumulation of greenhouse gas emissions have caused long-term changes in the global climate and environment

sparking widespread interest

in society for CO

bioconversion technologies as a means to address energy transition and climate change. As a new-generation biorefinery platform based on synthetic biology

the synthetic phototrophic community comprises closely cooperating phototrophic and heterotrophic microorganisms. This community is capable of efficiently converting light energy directly into biomass and a variety of chemicals through mutualistic metabolic division of labor among community members. Synthetic phototrophic community is one of the potential ways to achieve sustainable carbon-negative biomanufacturing

and has attracted widespread attention attributed to its advantages in applicability and robustness. In recent years

with the rapid development of systems biology and synthetic biotechnology

a variety of research efforts have been applied to the design and optimization of synthetic phototrophic communities

achieving stable progress and promoting the understanding of phototrophic community production. In this review

we briefly introduced an overview of the advances and current status of synthetic phototrophic community

including mutualistic mechanisms related to element

energy

and information flow. Subsequently

the unique advantages of phototrophic community were outlined. Meanwhile

recent systems biology approaches of phototrophic community were summarized

such as integrative analysis of multi-omics data

genome-scale metabolic modelling

flux balance analysis and community performance predictive algorithms. We also focused on the design and optimization strategies

such as chassis upgrading

immobilization/compartmentalization techniques

and enhanced internal multilayer regulation of synthetic phototrophic community

as well as the progress of their applications in various fields. Furthermore

we analyzed and discussed the constraints and challenges for the further deployment of synthetic phototrophic community on a larger scale

ranging from photosynthetic carbon production rate

intermediate organic matte

r selection

external predator invasion

to light distribution under high density cultivation. Finally

the future research strategies and engineering directions of synthetic phototrophic community encompassing semiconductor biohybrids

fine regulation of interspecies interaction and multi-omics community model construction were proposed. We conclude by providing a perspective on the future application scenarios of synthetic phototrophic communities in biochemistry

biomedicine

bioremediation and bioagriculture.

关键词

Keywords

references

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