Advances in the biological utilization of one-carbon compounds

LI Jian; CHEN Yun; LIU Haiyan; TAN Zaigao

doi:10.12211/2096-8280.2025-077

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Advances in the biological utilization of one-carbon compounds

更新时间：2025-10-13

- Advances in the biological utilization of one-carbon compounds
- Synthetic Biology Journal Pages: 1-18(2025)
- 作者机构：
  
  1.上海交通大学微生物代谢全国重点实验室，上海 200240
  2.上海交通大学生命科学技术学院生物工程系，上海 200240
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-077
  CLC： Q816
- Received：29 July 2025，
  
  Revised：2025-10-10，
  
  Online First：13 October 2025，
- 稿件说明：
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李健，陈云，刘海艳，谭在高. 一碳化合物生物利用的合成生物学研究进展［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-077

LI Jian， CHEN Yun， LIU Haiyan， TAN Zaigao. Advances in the biological utilization of one-carbon compounds［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-077
李健，陈云，刘海艳，谭在高. 一碳化合物生物利用的合成生物学研究进展［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-077 DOI：

LI Jian， CHEN Yun， LIU Haiyan， TAN Zaigao. Advances in the biological utilization of one-carbon compounds［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-077 DOI：

摘要

一碳化合物（C1，包括CO

、CO、甲烷、甲醇及甲酸）作为重要的含碳资源，凭借其来源广泛、经济及可再生特性，已成为生物制造领域的新型战略原料。近年来，合成生物学与代谢工程技术的突破性进展显著推动了C1化合物的生物转化路径创新，为碳中和目标下的绿色生物制造开辟了新范式。本文聚焦天然C1利用微生物的代谢网络优化、非天然C1利用途径的开发以及人工合成甲基营养菌的理性设计，系统比较评估了不同微生物底盘在C1生物转化中的应用潜力及关键C1同化途径的代谢特征，并综述了2022-2025年间该领域的前沿策略与技术成果。进一步对比分析了甲基营养菌的应用领域，探讨了各类宿主在产物合成中的适配性，探讨了当前存在的生物固碳效率低、人工固碳途径在体内难以高效运行、毒性中间体限制甲醇同化、人工甲基营养菌生长缓慢等难题。在此基础上，进一步讨论了实验室进化、酶工程、人工途径设计等多种手段协同提升C1利用效率的潜力，以及多学科交叉与人工智能在该领域发展中的重要作用，以期为C1驱动的可持续生物经济体系构建提供理论支撑。

Abstract

One-carbon (C1) compounds—including CO

methane

methanol

and formate—have emerged as strategic feedstocks for next-generation biomanufacturing owing to their abundance

economic viability

and renewability. However

the efficient biological conversion of C1 substrates into valuable products is hampered by several fundamental challenges

including the low intrinsic efficiency of natural carbon fixation pathways

the thermodynamic and kinetic barriers in engineering efficient

de novo

artificial assimilation routes

the cytotoxic effects of reactive intermediates like formaldehyde

and the generally suboptimal industrial robustness and slow growth of both native and synthetic C1-utilizing microbes. Recent breakthroughs in synthetic biology and metabolic

engineering have substantially mitigated these constraints

thereby accelerating C1 bioconversion and establishing a novel paradigm for carbon-neutral

green biomanufacturing. This review systematically examines state-of-the-art strategies and technological milestones reported between 2022 and 2025

with a focus on (i) Metabolic rewiring of native C1-utilizing microorganisms to enhance both C1-assimilation efficiency and product-synthesis capacity

(ii) de novo design of non-natural C1 assimilation pathways to provide more efficient route for the construction of C1-utilizing cell factories

and (iii) engineering artificial C1-utilizing cell factories through reconstituting natural or artificial C1 assimilation modules in well-established industrial fermentation strains to establish platform strains for C1-based bioproduction. Moving beyond strategy description

we provide a comparative analysis of the metabolic characteristics

advantages

and limitations of key natural and synthetic C1 assimilation pathways. We further evaluate the applicability of various microbial hosts for the synthesis of target products ranging from biofuels and bulk chemicals to specialized metabolites. A critical discussion addresses the persistent technical bottlenecks

such as low activity of key C1 assimilation enzymes

poor biomanufacturing capabilities of natural C1-utilizing bacteria

and the challenges in achieving high flux through artificial pathways

in vivo

. Finally

we explore the synergistic potential of integrated solutions—combining adaptive laboratory evolution

enzyme engineering

computational modeling

and systems-level analysis—to boost C1 utilization. We conclude by highlighting the transformative role of interdisciplinary convergence and artificial intelligence in accelerating the design-build-test-learn cycle

thereby paving the way for a sustainable

C1-driven bioeconomy.

关键词

Keywords

references

O'KEEFFE S , GARCIA L , CHEN Y , et al . Bringing carbon to life via one-carbon metabolism [J ] . Trends in Biotechnology , 2025 , 43 ( 3 ): 572 - 585 .

高教琪 , 周雍进 . 甲醇生物转化的机遇与挑战 [J ] . 合成生物学 , 2020 , 1 : 158 - 173 .

GAO J Q , ZHOU Y J . Advances in methanol bio-transformation [J ] . Synthetic biology Journal , 2020 , 1 : 572 - 585 .

MENG X , HU G , LI X , et al . A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts [J ] . Nature Communications , 2025 , 16 ( 1 ): 31

MENG Q , WANG D , FU X , et al . Converting Bacillus subtilis 168 to a synthetic methylotroph by combinatorial metabolic regulation strategies [J ] . Journal of Agricultural and Food Chemistry , 2025 , 73 ( 8 ): 4755 - 4763 .

NIEH L Y , CHEN F Y H , JUNG H W , et al . Evolutionar y engineering of methylotrophic E. coli enables fast growth on methanol [J ] . Nature Communications , 2024 , 15 ( 1 ): 8840 .

ZHAN C J , LI X , LAN G , et al . Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph [J ] . Nature Catalysis , 2023 , 6 ( 5 ): 435 - 450 .

REITER M A , BRADLEY T , BüCHEL L A , et al . A synthetic methylotrophic Escherichia coli as a chassis for bioproduction from methanol [J ] . Nature Catalysis , 2024 , 7 ( 5 ): 560 - 573 .

KRÜSEMANN J L , LINDNER S N . Bioproduction from methanol [J ] . Nature Catalysis , 2024 , 7 ( 5 ): 472 - 474 .

CAI P , WU X Y , DENG J , et al . Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris [J ] . Proceedings of the National Academy of Sciences , 2022 , 119 : e2201711119 .

MENG J , LIU S , GAO L , et al . Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale [J ] . Microbial Cell Factories , 2023 , 22 ( 1 ): 198 .

WU X Y , REN Y Y , CHEN S S , et al . Production of L-lactic acid from methanol by engineered yeast Pichia pastoris [J ] . Bioresource Technology , 2025 , 415 : 131730 .

SHEN Y W , CAI P , GAO L H , et al . Engineering high production of fatty alcohols from methanol by constructing coordinated dual biosynthetic pathways [J ] . Bioresource Technology , 2024 , 412 : 131396 .

ZHAI X X , GAO J Q , LI X Y , et al . Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast [J ] . Proceedings of the National Academy of Sciences , 2023 , 120 : e2220816120 .

WEFELMEIER K , SCHMITZ S , KöSTERS B J , et al . Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha [J ] . Microbial Cell Factories , 2024 , 23 ( 1 ): 8 .

KANG N K , CHAU T H T , LEE E Y . Engineered methane biocatalysis: strategies to assimilate methane for chemical production [J ] . Current Opinion in Biotechnology , 2024 , 85 : 103031 .

KELLER P , REITER M A , KIEFER P , et al . Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle [J ] . Nature Communications , 2022 , 13 : 5423 .

WENK S , RAINALDI V , SCHANN K , et al . Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO 2 via the serine threonine cycle [J ] . Metabolic Engineering , 2025 , 88 : 14 - 24 .

GREGORY G J , BENNETT R K , PAPOUTSAKIS E T . Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs [J ] . Metabolic Engineering , 2022 , 71 : 99 - 116 .

LIU L N . Advances in the bacterial organelles for CO 2 fixation [J ] . Trends in Microbiology , 2022 , 30 ( 6 ): 567 - 580 .

LUO S S , DIEHL C , HE H , et al . Construction and modular implementation of the THETA cycle for synthetic CO 2 fixation [J ] . Nature Catalysis , 2023 , 6 ( 12 ): 1228 - 1240 .

SATANOWSKI A , MARCHAL D G , PERRET A , et al . Design and implementation of aerobic and ambient CO 2 -reduction as an entry-point for enhanced carbon fixation [J ] . Nature Communications , 2025 , 16 ( 1 ): 3134 .

MCLEAN R , Schwander T , CHRISTOPH DIEHL , et al . Exploring alternative pathways for the in vitro establishment of the HOPAC cycle for synthetic CO 2 fixation [J ] . Science Advances , 2023 , 9 ( 24 ): eadh4299 .

LUO S S , LIN P P , NIEH LY , et al . A cell-free self-replenishing CO 2 -fixing system [J ] . Nature Catalysis , 2022 , 5 ( 2 ): 154 - 162 .

FENG J , LI X , TENG X , et al . Harnessing CO 2 fixation and reducing power recycling for enhanced polyhydroxyalkanoates industrial bioproduction [J ] . Metabolic Engineering , 2025 , 91 : 204 - 216 .

ZHU P , CHEN X . Converting heterotrophic Escherichia coli into synthetic C1-trophic modes [J ] . Trends in Chemistry , 2022 , 4 ( 10 ): 860 - 862 .

LI C , ZHENG H , LI Y , et al . Facilitated channeling of fixed carbon and energy into chemicals in artificial phototrophic communities [J ] . Journal of the American Chemical Society , 2025 , 147 ( 6 ): 4707 - 4713 .

FENG J , MA D , GAO S , et al . Recent advances in engineering heterotrophic microorganisms for reinforcing CO 2 fixation based on Calvin-Benson-Bassham Cycle [J ] . ACS Sustainable Chemistry & Engineering , 2023 , 11 ( 26 ): 9509 - 9522 .

PENG J H , LO S C , YU Y N , et al . Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition [J ] . Journal of Biological Engineering , 2025 , 19 ( 1 ): 20 .

CUI Z Y , ZHONG Y T , SUN Z J , et al . Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica [J ] . Nature Communications , 2023 , 14 : 8480 .

PAN J , ZHANG X X , XU W , et al . Wood–Ljungdahl pathway found in novel marine Korarchaeota groups illuminates their evolutionary history [J ] . Msystems , 2023 , 8 ( 4 ): e00305-23 .

MATTOZZI M D , ZIESACK M , VOGES M J , et al . Expression of the sub-pathways of the Chloroflexus aurantiacus 3-hydroxypropionate carbon fixation bicycle in E. coli : Toward horizontal transfer of autotrophic growth [J ] . Metabolic Engineering , 2013 , 16 : 130 - 139 .

QIN N , LI L Y , WAN X Z , et al . Increased CO 2 fixation enables high carbon-yield production of 3-hydroxypropionic acid in yeast [J ] . Nature Communications , 2024 , 15 : 1591 .

SIMONE GIAVERI , NITIN BOHRA , CHRISTOPH DIEHL , et al . Integrated translation and metabolism in a partially self-synthesizing biochemical network [J ] . Science , 385 ( 6705 ): 174 - 178 .

HARALD H , Martin G , ULRIKE J , et al . A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis [J ] . Proceedings of the National Academy of Sciences , 2008 , 105 ( 22 ): 7851 - 7856 .

SARWAR A , LEE E Y . Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs [J ] . Synthetic and Systems Biotechnology , 2023 , 8 ( 3 ): 396 - 415 .

YISHAI O , LINDNER S N , GONZALEZ DE LA CRUZ J , et al . The formate bio-economy [J ] . Current Opinion in Chemical Biology , 2016 , 35 : 1 - 9 .

JIA M , LIU M , LI J , et al . Formaldehyde: an essential intermediate for C1 metabolism and bioconversion [J ] . ACS Synthetic Biology , 2024 , 13 ( 11 ): 3507 - 3522 .

WANG Y Y , LI R S , ZHAO F G , et al . Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol [J ] . Microbial Cell Factories , 2024 , 23 ( 1 ): 198 .

DRONSELLA B , ORSI E , SCHULZ-MIRBACH H , et al . One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle [J ] . Nature Microbiology , 2025 , 10 ( 3 ): 646 - 653 .

BYSANI V R , ALAM A S , BAR-EVEN A , et al . Engineering and evolution of the complete reductive glycine pathway in Saccharomyces cerevisiae for formate and CO 2 assimilation [J ] . Metabolic Engineering , 2024 , 81 : 167 - 181 .

CHOU A , LEE S H , ZHU F , et al . An orthogonal metabolic framework for one-carbon utilization [J ] . Nature Metabolism , 2021 , 3 ( 10 ): 1385 - 1399 .

SEUNG HWAN LEE A C , MAREN NATTERMANN , FAYIN ZHU , JAMES M. CLOMBURG , NICOLE PACZIA , TOBIAS J. ERB , RAMON GONZALEZ . Identification of 2-hydroxyacyl-CoA synthases with high acyloin condensation activity for orthogonal one-carbon bioconversion [J ] . ACS Catalysis , 2023 , 13 : 12007 - 12020 .

WU T , GÓMEZ-CORONADO P A , KUBIS A , et al . Engineering a synthetic energy-efficient formaldehyde assimilation cycle in Escherichia coli [J ] . Nature Communications , 2023 , 14 ( 1 ): 8490 .

LU X Y , LIU Y W , YANG Y Y , et al . Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design [J ] . Nature Communications , 2019 , 10 ( 1 ): 1378 .

DANG B T , BUI X T , TRAN D P H , et al . Current application of algae derivatives for bioplastic production: A review [J ] . Bioresource Technology , 2022 , 347 : 126698 .

MEHDIZADEH ALLAF M , PEERHOSSAINI H . Cyanobacteria: model microorganisms and beyond [J ] . Microorganisms , 2022 , 10 ( 4 ): 696 .

PESSOA J D S , DE OLIVEIRA C F M , MENA‐CHALCO J P , et al . Trends on Chlamydomonas reinhardtii growth regimes and bioproducts [J ] . Biotechnology and Applied Biochemistry , 2023 , 70 ( 6 ): 1830 - 1842 .

DONATI S , JOHNSON C W . Optimizing Cupriavidus necator H16 as a host for aerobic C1 conversion [J ] . Current Opinion in Biotechnology , 2025 , 93 .

JIANG J , LI X , YANG K , et al . Photosynthetic cultivation of Chlamydomonas reinhardti i with formate as a novel carbon source to the protein production [J ] . Chemical Engineering Journal , 2024 , 493 : 152518 .

MORLINO M S , SERNA GARCíA R , SAVIO F , et al . Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches [J ] . Biotechnology Advances , 2023 , 69 : 108264 .

MADHU S , SENGUPTA A , SARNAIK A P , et al . Expanding the synthe tic biology repertoire of a fast‐growing cyanobacterium Synechococcus elongatus PCC 11801 [J ] . Biotechnology and Bioengineering , 2024 , 121 ( 9 ): 2974 - 2980 .

LI Z , LI S , CHEN L , et al . Fast-growing cyanobacterial chassis for synthetic biology application [J ] . Critical Reviews in Biotechnology , 2023 , 44 ( 3 ): 414 - 428 .

SANTOS-MERINO M , GUTIéRREZ-LANZA R , NOGALES J , et al . Synechococcus elongatus PCC 7942 as a platform for bioproduction of omega-3 fatty acids [J ] . Life , 2022 , 12 ( 6 ): 810 .

PRITAM P , SARNAIK A P , WANGIKAR P P . Metabolic engineering of Synechococcus elongatus for photoautotrophic production of mannitol [J ] . Biotechnology and Bioengineering , 2023 , 120 ( 8 ): 2363 - 2370 .

WANG B , Cristal Zuniga , GUARNIERI M T , et al . Metabolic engineering of Synechococcus elongatus 7942 for enhanced sucrose biosynthesis [J ] . Metabolic Engineering , 2023 , 80 : 12 - 24 .

RAUTELA A , YADAV I , GANGWAR A , et al . Photosynthetic production of α-farnesene by engineered Synechococcus elongatus UTEX 2973 from carbon dioxide [J ] . Bioresource Technology , 2024 , 396 : 130432 .

MA K , DENG L , WU H Z , et al . Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast [J ] . Bioresources and Bioprocessing , 2022 , 9 ( 1 ): 1 - 14 .

MASI A , LEONELLI F , SCOGNAMIGLIO V , et al . Chlamydomonas reinhardtii: a factory of nutraceutical and food supplements for human health [J ] . Molecules , 2023 , 28 ( 3 ): 1185 .

DELLA VALLE S , ORSI E , CREUTZBURG S C A , et al . Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system [J ] . Trends in Biotechnology , 2025 , 43 : 1470 - 1491 .

YANG X T , ZHENG Z J , WANG Y . Bacillus methanolicus : an emerging chassis for low-carbon biomanufacturing [J ] . Trends in Biotechnology , 2025 , 43 ( 2 ): 274 - 277 .

XIE L F , YU W , GAO J Q , et al . Ogataea polymorpha as a next-generation chassis for industrial biotechnology [J ] . Trends in Biotechnology , 2024 , 42 ( 11 ): 1363 - 1378 .

GUO F , QIAO Y , XIN F , et al . Bioconversion of C1 feedstocks for chemical production using Pichia pastoris [J ] . Trends in Biotechnology , 2023 , 41 ( 8 ): 1066 - 1079 .

WU X Y , CAI P , YAO L , et al . Genetic tools for metabolic engineering of Pichia pastoris [J ] . Engineering Microbiology , 2023 , 3 ( 4 ): 100094 .

OCHSNER A M , SONNTAG F , BUCHHAUPT M , et al . Methylobacterium extorquens : methylotrophy and biotechnological applications [J ] . Applied Microbiology and Biotechnology , 2014 , 99 ( 2 ): 517 - 534 .

MüLLER J E N , HEGGESET T M B , WENDISCH V F , et al . Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and applicati on for commodity production from methanol [J ] . Applied Microbiology and Biotechnology , 2014 , 99 ( 2 ): 535 - 551 .

NI X , ZHAI X X , YU W , et al . Dynamically regulating homologous recombination enables precise genome editing in Ogataea polymorpha [J ] . ACS Synthetic Biology , 2024 , 13 ( 9 ): 2938 - 2947 .

JIA W , POUVREAU L , VAN DER GOOT A J , et al . Renewable methanol utilizing bacteria as future meat analogue: An explorative study on the physicochemical and texturing properties of Methylobacillus flagellatus biomass and fractions [J ] . Food Hydrocolloids , 2024 , 151 : 109832 .

XU D Y , LEUNG K M , LAI G K K , et al . Complete genome sequence of Klebsiella pneumoniae RX.G5M15, a methanol-metabolizing strain recovered from the sole of a shoe [J ] . Microbiology Resource Announcements , 2023 , 13 ( 9 ): e00451-24 .

PONTRELLI S , CHIU T Y , LAN E I , et al . Escherichia coli as a host for metabolic engineering [J ] . Metabolic Engineering , 2018 , 50 : 16 - 46 .

SANFORD P A , WOOLSTON B M . Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol [J ] . Current Opinion in Biotechnology , 2022 , 74 : 171 - 179 .

WEGAT V , FABARIUS J T , SIEBER V . Synthetic methylotrophic yeasts for the sustainable fuel and chemical production [J ] . Biotechnology for Biofuels and Bioproducts , 2022 , 15 : 113 .

DELMAS V A , PERCHAT N , MONET O , et al . Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture [J ] . Metabolic Engineering , 2022 , 72 : 200 - 214 .

SUN Q , LIU D , CHEN Z . Engineering and adaptive laboratory evolution of Escherichia coli for improving methanol utilization based on a hybrid methanol assimilation pathway [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 10 : 1089639 .

GUO Y , ZHANG R , WANG J , et al . Engineering yeasts to Co-utilize methanol or formate coupled with CO 2 fixation [J ] . Metabolic Engineering , 2024 , 84 : 1 - 12 .

QI M , ZHU C , CHENG C , et al . Rewiring methanol assimilation and reductive glycine pathways in Saccharomyces cerevisiae to increase one-carbon recovery [J ] . Green Chemistry , 2025 , 27 ( 12 ): 3261 - 3271 .

LIU D , WANG L , Gou L B , et al . Hybrid Methylotrophic pathway in Serratia marcescens for sustainable terpenoid biosynthesis [J ] . ACS Synthetic Biology , 2025 , 14 ( 5 ): 1766 - 1776 .

ZHANG S , GUO F , YANG Q , et al . Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization [J ] . Green Chemistry , 2023 , 25 ( 1 ): 183 - 195 .

GAO B , ZHAO N , DENG J , et al . Constructing a methanol-dependent Bacillus subtilis by engineering the methanol metabolism [J ] . Journal of Biotechnology , 2022 , 343 : 128 - 137 .

YANG J G , SONG W , CAI T , et al . De novo artificial synthesis of hexoses from carbon dioxide [J ] . Science Bulletin , 2023 , 68 ( 20 ): 2370 - 2381 .

WANG Y Y , CHEN P , LI W W , et al . Cell-free synthesis of high-order carbohydrates from low-carbon molecules [J ] . Science Bulletin , 2025 , 70 ( 14 ): 2266 - 2276 .

YUN L , ZEGARAC R , DUCAT D C . Impact of irradiance and inorganic carbon availability on heterologous sucrose production in Synechococcus elongatus PCC 7942 [J ] . Frontiers in Plant Science , 2024 , 15 : 1378573 .

ZHAO M L , CAI W S , ZHENG S Q , et al . Metabolic engineering of the isopentenol utilization pathway enh anced the production of terpenoids in Chlamydomonas reinhardtii [J ] . Marine Drugs , 2022 , 20 ( 9 ): 577 .

AMENDOLA S , KNEIP J S , MEYER F , et al . Metabolic engineering for efficient ketocarotenoid accumulation in the green microalga Chlamydomonas reinhardtii [J ] . ACS Synthetic Biology , 2023 , 12 ( 3 ): 820 - 831 .

LIN J Y , SRI WAHYU EFFENDI S , NG I S . Enhanced carbon capture and utilization (CCU) using heterologous carbonic anhydrase in Chlamydomonas reinhardtii for lutein and lipid production [J ] . Bioresource Technology , 2022 , 351 : 127009 .

KANG N K , KOH H G , CHOI Y , et al . Bioconversion of CO 2 into valuable bioproducts via synthetic modular co-culture of engineered Chlamydomonas reinhardtii and Escherichia coli [J ] . Metabolic Engineering , 2025 , 90 : 57 - 66 .

WANG X L , CHANG F F , WANG T T , et al . Production of N-acetylglucosamine from carbon dioxide by engineering Cupriavidus necator H16 [J ] . Bioresource Technology , 2023 , 379 : 129024 .

WANG X L , WANG K K , WANG L , et al . Engineering Cupriavidus necator H16 for heterotrophic and autotrophic production of myo-inositol [J ] . Bioresource Technology , 2023 , 368 : 128321 .

WANG L , YAO J H , TU T , et al . Heterotrophic and autotrophic production of L-isoleucine and L-valine by engineered Cupriavidus necator H16 [J ] . Bioresource Technology , 2024 , 398 : 130538 .

LI C F , WANG R Y , WANG J W , et al . A highly compatible phototrophic community for carbon‐negative biosynthesis [J ] . Angewandte Chemie International Edition , 2022 .

LI C F , YIN L , WANG J W , et al . Light-driven biosynthesis of volatile, unstable and photosensitive chemicals from CO 2 [J ] . Nature Synthesis , 2023 , 2 ( 10 ): 960 - 971 .

WANG J , LIAO Y , QIN J , et al . Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum [J ] . Biotechnology for Biofuels and Bioproducts , 2023 , 16 : 10 .

GAO L , HOU R , CAI P , et al . Engineering yeast peroxisomes for α-bisabolene production from sole methanol with the aid of proteomic analysis [J ] . JACS Au , 2024 , 4 ( 7 ): 2474 - 2483 .

NIU T , YAN X , WANG J , et al . Engineering of Pichia pastoris for the de novo synthesis of the sesquiterpene zealexin a1 from methanol [J ] . ACS Sustainable Chemistry & Engineering , 2024 , 12 ( 34 ): 12786 - 12794 .

INOUE Y , YAMADA R , MATSUMOTO T , et al . Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii [J ] . Biotechnology for Biofuels and Bioproducts , 2024 , 17 ( 1 ): 149 .

SEVERINSEN M M , BACHLEITNER S , MODENESE V , et al . Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii [J ] . Biotechnology for Biofuels and Bioproducts , 2024 , 17 ( 1 ): 98 .

WANG S X , FANG J Y , WANG M Y , et al . Rewiring the me thanol assimilation pathway in the methylotrophic yeast Pichia pastoris for high-level production of erythritol [J ] . Bioresource Technology , 2025 , 427 : 132430 .

ZHAO B J , LI Y , ZHANG Y , et al . Low-carbon and overproduction of cordycepin from methanol using engineered Pichia pastoris cell factory [J ] . Bioresource Technology , 2024 , 413 : 131446 .

ÀVILA-CABRé S , ALBIOL J , FERRER P . Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol [J ] . Journal of Biological Engineering , 2025 , 19 : 19 .

MA Z X , FENG C X , SONG Y Z , et al . Engineering photo-methylotrophic Methylobacterium for enhanced 3-hydroxypropionic acid production during non-growth stage fermentation [J ] . Bioresource Technology , 2024 , 393 : 130104 .

CHANG W , YOON J , OH M-K . Production of polyhydroxyalkanoates with the fermentation of Methylorubrum extorquens using formate as a carbon substrate [J ] . Biotechnology and Bioprocess Engineering , 2022 , 27 ( 2 ): 268 - 275 .

LI B , YANG Z , LI Z , et al . Enabling genetic manipulation and robustness of Bacillus methanolicus for methanol-based bio-manufacturing [J ] . Metabolic Engineering , 2025 , 89 : 121 - 134 .

GUO Q , ZHENG L J , ZHENG S H , et al . Enhanced biosynthesis of D-allulose from a D-xylose-methanol mixture and its self-inductive detoxification by using antisense RNAs in Escherichia coli [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 26 ): 14821 - 14829 .

KIM S , GIRALDO N , RAINALDI V , et al . Optimizing E. coli as a formatotrophic platform for bioproduction via the reductive glycine pathway [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1091899 .

LI M K , SUN W J , WANG X , et al . A eukaryote-featured membrane phospholipid enhances bacterial formaldehyde tolerance and assimilation of one-carbon feedstocks [J ] . ACS Synthetic Biology , 2024 , 13 ( 12 ): 4074 - 4084 .

CHEN W X , ZHWNF L J , LUO X , et al . Metabolic engineering and adaptive evolution of Escherichia coli for enhanced conversion of d‑xylose to d-glucaric acid mediated by methanol [J ] . Biotechnology and Bioengineering , 2025 , 122 : 1472 - 1483 .

YU W , GAO J Q , YAO L , et al . Bioconversion of methanol to 3-hydroxypropionate by engineering Ogataea polymorpha [J ] . Chinese Journal of Catalysis , 2023 , 46 : 84 - 90 .

GAO J Q , LI Y , YU W , et al . Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol [J ] . Nature Metabolism , 2022 , 4 ( 7 ): 932 - 943 .

WEFELMEIER K , SCHMITZ S , HAUT A M , et al . Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1223726 .

LIU H Y , CHEN Y , LI J , et al . Scavenging intracellular reactive oxygen species to boost methanol assimilation [J ] . Chemical Engineering Journal , 2025 , 516 : 164002 .

ZHU C , CHEN Y , SUN W J , et al . Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation [J ] . Fundamental Research , 2025 .

GUO F , LIU K , QIAO Y Y , et al . Evolutionary engineering of S accharomyces cerevisiae : Crafting a synthetic methylotroph via self-reprogramming [J ] . Science Advances , 2024 , 10 : 1 - 15 .

QIAN J , FAN L , YANG J , et al . Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde [J ] . Synthetic and Systems Biotechnology , 2023 , 8 ( 3 ): 386 - 395 .

PHAM D N , NGUYEN A D , LEE E Y . Outlook on engineering methylotrophs for one-carbon-based industrial biotechnology [J ] . Chemical Engineering Journal , 2022 , 449 : 137769 .

LI J J , GAO J Q , YE M , et al . Engineering yeast for high-level production of β-farnesene from sole methanol [J ] . Metabolic Engineering , 2024 , 85 : 194 - 200 .

SRISAWAT P , HIGUCHI-TAKEUCHI M , NUMATA K . Microbial autotrophic biorefineries: Perspectives for biopolymer production [J ] . polymer journal , 2022 , 54 ( 10 ): 1139 - 1151 .

SHIN J , BAE J , LEE H , et al . Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum [J ] . Proceedings of the National Academy of Sciences , 2023 , 120 ( 6 ): e2216244120 .

LI J , ZHANF L Y , XU Q , et al . CRISPR-cas9 toolkit for genome editing in an autotrophic CO 2 -fixing Methanogenic Archaeon [J ] . Microbiology Spectrum , 2022 , 10 ( 4 ): e01165-22 .

LEE J , YU H E , LEE S Y . Metabolic engineering of microorganisms for carbon dioxide utilization [J ] . Current Opinion in Biotechnology , 2025 , 91 : 103244 .

MICHAEL BAUMSCHABL , Özge Ata , BERND M . MITIC, et al. Conversion of CO2 into organic acids by engineered autotrophic yeast [J ] . Proceedings of the National Academy of Sciences , 2022 , 119 ( 47 ): e2211827119 .

YU M J , LI M L , ZHANG X Z , et al . Coupling Photocatalytic Reduction and Biosynthesis Towards Sustainable CO 2 Upcycling [J ] . Angewandte Chemie International Edition , 2025 , 64 ( 20 ): e202423995 .

GEWEDA A E , ZAYED M E , KHAN M Y , et al . Mitigating CO 2 emissions: A review on emerging technologies/strategies for CO 2 capture [J ] . Journal of the Energy Institute , 2025 , 118 : 101911 .

TEDEEVA M A , KUSTOV A L , BATKIN A M , et al . Catalytic systems for hydrogenation of CO 2 to methanol [J ] . Molecular Catalysis , 2024 , 566 : 114403 .

WEI C J , DING H L , ZHANG Z Y , et al . Research progress of bimetallic catalysts for CO 2 hydrogenation to methane [J ] . Int J Hydrogen Energ , 2024 , 58 : 872 - 891 .

GAN Y M , MENG X , GAO C , et al . Metabolic engineering strategies for microbial utilization of methanol [J ] . Engineering Microbiology , 2023 , 3 : 100081 .

PACESA M , PELEA O , JINEK M . Past, present, and future of CRISPR genome editing technologies [J ] . Cell , 2024 , 187 ( 5 ): 1076 - 1100 .

DIXIT S , KUMAR A , SRINIVASAN K , et al . Advancing genome editing with artificial intelligence: opportunities, challenges, and future directions [J ] . Frontiers in Bioengineering and Biotechnology , 2024 , 11 : 1335901 .

PETZOLD C J , MUKHOPADHYAY A . From bench to biofactory: high-throughput technologies and automated workflows to accelerate biomanufacturing [J ] . Current Opinion in Biotechnology , 2025 , 94 : 103320 .

LANDWEHR G M , BOGART J W , MAGALHAES C , et al . Accelerated enzyme engineering by machine-learning guided cell-free expression [J ] . Nature Communications , 2025 , 16 : 865 .

WATSON J L , JUERGENS D , BENNETT N R , et al . De novo design of protein structure and function with RFdiffusion [J ] . Nature , 2023 , 620 ( 7976 ): 1089 - 1100 .

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